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Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy

The altered glucose metabolism characterising cancer cells determines an increased amount of methylglyoxal in their secretome. Previous studies have demonstrated that the methylglyoxal, in turn, modifies the protonation state (PS) of soluble proteins contained in the secretomes of cultivated circula...

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Autores principales: Bonapace, Giuseppe, Gentile, Francesco, Coppedé, Nicola, Coluccio, Maria Laura, Garo, Virginia, Vismara, Marco Flavio Michele, Candeloro, Patrizio, Donato, Giuseppe, Malara, Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472697/
https://www.ncbi.nlm.nih.gov/pubmed/34578748
http://dx.doi.org/10.3390/nano11092432
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author Bonapace, Giuseppe
Gentile, Francesco
Coppedé, Nicola
Coluccio, Maria Laura
Garo, Virginia
Vismara, Marco Flavio Michele
Candeloro, Patrizio
Donato, Giuseppe
Malara, Natalia
author_facet Bonapace, Giuseppe
Gentile, Francesco
Coppedé, Nicola
Coluccio, Maria Laura
Garo, Virginia
Vismara, Marco Flavio Michele
Candeloro, Patrizio
Donato, Giuseppe
Malara, Natalia
author_sort Bonapace, Giuseppe
collection PubMed
description The altered glucose metabolism characterising cancer cells determines an increased amount of methylglyoxal in their secretome. Previous studies have demonstrated that the methylglyoxal, in turn, modifies the protonation state (PS) of soluble proteins contained in the secretomes of cultivated circulating tumour cells (CTCs). In this study, we describe a method to assess the content of methylglyoxal adducts (MAs) in the secretome by near-infrared (NIR) portable handheld spectroscopy and the extreme learning machine (ELM) algorithm. By measuring the vibration absorption functional groups containing hydrogen, such as C-H, O-H and N-H, NIR generates specific spectra. These spectra reflect alterations of the energy frequency of a sample bringing information about its MAs concentration levels. The algorithm deciphers the information encoded in the spectra and yields a quantitative estimate of the concentration of MAs in the sample. This procedure was used for the comparative analysis of different biological fluids extracted from patients suspected of having cancer (secretome, plasma, serum, interstitial fluid and whole blood) measured directly on the solute left on a surface upon a sample-drop cast and evaporation, without any sample pretreatment. Qualitative and quantitative regression models were built and tested to characterise the different levels of MAs by ELM. The final model we selected was able to automatically segregate tumour from non-tumour patients. The method is simple, rapid and repeatable; moreover, it can be integrated in portable electronic devices for point-of-care and remote testing of patients.
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spelling pubmed-84726972021-09-28 Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy Bonapace, Giuseppe Gentile, Francesco Coppedé, Nicola Coluccio, Maria Laura Garo, Virginia Vismara, Marco Flavio Michele Candeloro, Patrizio Donato, Giuseppe Malara, Natalia Nanomaterials (Basel) Article The altered glucose metabolism characterising cancer cells determines an increased amount of methylglyoxal in their secretome. Previous studies have demonstrated that the methylglyoxal, in turn, modifies the protonation state (PS) of soluble proteins contained in the secretomes of cultivated circulating tumour cells (CTCs). In this study, we describe a method to assess the content of methylglyoxal adducts (MAs) in the secretome by near-infrared (NIR) portable handheld spectroscopy and the extreme learning machine (ELM) algorithm. By measuring the vibration absorption functional groups containing hydrogen, such as C-H, O-H and N-H, NIR generates specific spectra. These spectra reflect alterations of the energy frequency of a sample bringing information about its MAs concentration levels. The algorithm deciphers the information encoded in the spectra and yields a quantitative estimate of the concentration of MAs in the sample. This procedure was used for the comparative analysis of different biological fluids extracted from patients suspected of having cancer (secretome, plasma, serum, interstitial fluid and whole blood) measured directly on the solute left on a surface upon a sample-drop cast and evaporation, without any sample pretreatment. Qualitative and quantitative regression models were built and tested to characterise the different levels of MAs by ELM. The final model we selected was able to automatically segregate tumour from non-tumour patients. The method is simple, rapid and repeatable; moreover, it can be integrated in portable electronic devices for point-of-care and remote testing of patients. MDPI 2021-09-18 /pmc/articles/PMC8472697/ /pubmed/34578748 http://dx.doi.org/10.3390/nano11092432 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bonapace, Giuseppe
Gentile, Francesco
Coppedé, Nicola
Coluccio, Maria Laura
Garo, Virginia
Vismara, Marco Flavio Michele
Candeloro, Patrizio
Donato, Giuseppe
Malara, Natalia
Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy
title Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy
title_full Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy
title_fullStr Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy
title_full_unstemmed Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy
title_short Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy
title_sort methylglyoxal adducts levels in blood measured on dried spot by portable near-infrared spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472697/
https://www.ncbi.nlm.nih.gov/pubmed/34578748
http://dx.doi.org/10.3390/nano11092432
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