Cargando…

Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities

Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show hi...

Descripción completa

Detalles Bibliográficos
Autores principales: Martínez de Toda, Irene, Rattan, Suresh I. S., De la Fuente, Mónica, Arranz, Lorena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472714/
https://www.ncbi.nlm.nih.gov/pubmed/34573029
http://dx.doi.org/10.3390/antiox10091397
_version_ 1784574805664595968
author Martínez de Toda, Irene
Rattan, Suresh I. S.
De la Fuente, Mónica
Arranz, Lorena
author_facet Martínez de Toda, Irene
Rattan, Suresh I. S.
De la Fuente, Mónica
Arranz, Lorena
author_sort Martínez de Toda, Irene
collection PubMed
description Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show high levels. However, contradictory results exist depending on the tissue or cell type and it is unknown how the 20S proteasome functions in exceptionally old mice. The aim of this study was to investigate two proteasome activities (caspase-like and chymotrypsin-like) in several tissues (lung, heart, axillary lymph nodes, liver, kidney) and cells (peritoneal leukocytes) from adult (28 ± 4 weeks, n = 12), old (76 ± 4 weeks, n = 9) and exceptionally old (128 ± 4 weeks, n = 9) BALB/c female mice. The results show different age-related changes depending on the tissue and the activity considered, so there is no universal decline in proteasome function with age in female mice. Interestingly, exceptionally old mice displayed better maintained proteasome activities, suggesting that preserved 20S proteasome is associated with successful aging.
format Online
Article
Text
id pubmed-8472714
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84727142021-09-28 Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities Martínez de Toda, Irene Rattan, Suresh I. S. De la Fuente, Mónica Arranz, Lorena Antioxidants (Basel) Brief Report Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show high levels. However, contradictory results exist depending on the tissue or cell type and it is unknown how the 20S proteasome functions in exceptionally old mice. The aim of this study was to investigate two proteasome activities (caspase-like and chymotrypsin-like) in several tissues (lung, heart, axillary lymph nodes, liver, kidney) and cells (peritoneal leukocytes) from adult (28 ± 4 weeks, n = 12), old (76 ± 4 weeks, n = 9) and exceptionally old (128 ± 4 weeks, n = 9) BALB/c female mice. The results show different age-related changes depending on the tissue and the activity considered, so there is no universal decline in proteasome function with age in female mice. Interestingly, exceptionally old mice displayed better maintained proteasome activities, suggesting that preserved 20S proteasome is associated with successful aging. MDPI 2021-08-31 /pmc/articles/PMC8472714/ /pubmed/34573029 http://dx.doi.org/10.3390/antiox10091397 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Martínez de Toda, Irene
Rattan, Suresh I. S.
De la Fuente, Mónica
Arranz, Lorena
Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
title Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
title_full Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
title_fullStr Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
title_full_unstemmed Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
title_short Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
title_sort female mice reaching exceptionally high old age have preserved 20s proteasome activities
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472714/
https://www.ncbi.nlm.nih.gov/pubmed/34573029
http://dx.doi.org/10.3390/antiox10091397
work_keys_str_mv AT martinezdetodairene femalemicereachingexceptionallyhigholdagehavepreserved20sproteasomeactivities
AT rattansureshis femalemicereachingexceptionallyhigholdagehavepreserved20sproteasomeactivities
AT delafuentemonica femalemicereachingexceptionallyhigholdagehavepreserved20sproteasomeactivities
AT arranzlorena femalemicereachingexceptionallyhigholdagehavepreserved20sproteasomeactivities