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Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities
Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show hi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472714/ https://www.ncbi.nlm.nih.gov/pubmed/34573029 http://dx.doi.org/10.3390/antiox10091397 |
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author | Martínez de Toda, Irene Rattan, Suresh I. S. De la Fuente, Mónica Arranz, Lorena |
author_facet | Martínez de Toda, Irene Rattan, Suresh I. S. De la Fuente, Mónica Arranz, Lorena |
author_sort | Martínez de Toda, Irene |
collection | PubMed |
description | Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show high levels. However, contradictory results exist depending on the tissue or cell type and it is unknown how the 20S proteasome functions in exceptionally old mice. The aim of this study was to investigate two proteasome activities (caspase-like and chymotrypsin-like) in several tissues (lung, heart, axillary lymph nodes, liver, kidney) and cells (peritoneal leukocytes) from adult (28 ± 4 weeks, n = 12), old (76 ± 4 weeks, n = 9) and exceptionally old (128 ± 4 weeks, n = 9) BALB/c female mice. The results show different age-related changes depending on the tissue and the activity considered, so there is no universal decline in proteasome function with age in female mice. Interestingly, exceptionally old mice displayed better maintained proteasome activities, suggesting that preserved 20S proteasome is associated with successful aging. |
format | Online Article Text |
id | pubmed-8472714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84727142021-09-28 Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities Martínez de Toda, Irene Rattan, Suresh I. S. De la Fuente, Mónica Arranz, Lorena Antioxidants (Basel) Brief Report Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show high levels. However, contradictory results exist depending on the tissue or cell type and it is unknown how the 20S proteasome functions in exceptionally old mice. The aim of this study was to investigate two proteasome activities (caspase-like and chymotrypsin-like) in several tissues (lung, heart, axillary lymph nodes, liver, kidney) and cells (peritoneal leukocytes) from adult (28 ± 4 weeks, n = 12), old (76 ± 4 weeks, n = 9) and exceptionally old (128 ± 4 weeks, n = 9) BALB/c female mice. The results show different age-related changes depending on the tissue and the activity considered, so there is no universal decline in proteasome function with age in female mice. Interestingly, exceptionally old mice displayed better maintained proteasome activities, suggesting that preserved 20S proteasome is associated with successful aging. MDPI 2021-08-31 /pmc/articles/PMC8472714/ /pubmed/34573029 http://dx.doi.org/10.3390/antiox10091397 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Martínez de Toda, Irene Rattan, Suresh I. S. De la Fuente, Mónica Arranz, Lorena Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities |
title | Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities |
title_full | Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities |
title_fullStr | Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities |
title_full_unstemmed | Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities |
title_short | Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities |
title_sort | female mice reaching exceptionally high old age have preserved 20s proteasome activities |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472714/ https://www.ncbi.nlm.nih.gov/pubmed/34573029 http://dx.doi.org/10.3390/antiox10091397 |
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