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Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses

To date, no evidence supports the fact that animals play a role in the epidemiology of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus infectious disease 2019 (COVID-19). However, several animal species are naturally susceptible to SARS-CoV-2...

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Autores principales: Fernández-Bellon, Hugo, Rodon, Jordi, Fernández-Bastit, Leira, Almagro, Vanessa, Padilla-Solé, Pilar, Lorca-Oró, Cristina, Valle, Rosa, Roca, Núria, Grazioli, Santina, Trogu, Tiziana, Bensaid, Albert, Carrillo, Jorge, Izquierdo-Useros, Nuria, Blanco, Julià, Parera, Mariona, Noguera-Julián, Marc, Clotet, Bonaventura, Moreno, Ana, Segalés, Joaquim, Vergara-Alert, Júlia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472846/
https://www.ncbi.nlm.nih.gov/pubmed/34578266
http://dx.doi.org/10.3390/v13091683
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author Fernández-Bellon, Hugo
Rodon, Jordi
Fernández-Bastit, Leira
Almagro, Vanessa
Padilla-Solé, Pilar
Lorca-Oró, Cristina
Valle, Rosa
Roca, Núria
Grazioli, Santina
Trogu, Tiziana
Bensaid, Albert
Carrillo, Jorge
Izquierdo-Useros, Nuria
Blanco, Julià
Parera, Mariona
Noguera-Julián, Marc
Clotet, Bonaventura
Moreno, Ana
Segalés, Joaquim
Vergara-Alert, Júlia
author_facet Fernández-Bellon, Hugo
Rodon, Jordi
Fernández-Bastit, Leira
Almagro, Vanessa
Padilla-Solé, Pilar
Lorca-Oró, Cristina
Valle, Rosa
Roca, Núria
Grazioli, Santina
Trogu, Tiziana
Bensaid, Albert
Carrillo, Jorge
Izquierdo-Useros, Nuria
Blanco, Julià
Parera, Mariona
Noguera-Julián, Marc
Clotet, Bonaventura
Moreno, Ana
Segalés, Joaquim
Vergara-Alert, Júlia
author_sort Fernández-Bellon, Hugo
collection PubMed
description To date, no evidence supports the fact that animals play a role in the epidemiology of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus infectious disease 2019 (COVID-19). However, several animal species are naturally susceptible to SARS-CoV-2 infection. Besides pets (cats, dogs, Syrian hamsters, and ferrets) and farm animals (minks), different zoo animal species have tested positive for SARS-CoV-2 (large felids and non-human primates). After the summer of 2020, a second wave of SARS-CoV-2 infection occurred in Barcelona (Spain), reaching a peak of positive cases in November. During that period, four lions (Panthera leo) at the Barcelona Zoo and three caretakers developed respiratory signs and tested positive for the SARS-CoV-2 antigen. Lion infection was monitored for several weeks and nasal, fecal, saliva, and blood samples were taken at different time-points. SARS-CoV-2 RNA was detected in nasal samples from all studied lions and the viral RNA was detected up to two weeks after the initial viral positive test in three out of four animals. The SARS-CoV-2 genome was also detected in the feces of animals at different times. Virus isolation was successful only from respiratory samples of two lions at an early time-point. The four animals developed neutralizing antibodies after the infection that were detectable four months after the initial diagnosis. The partial SARS-CoV-2 genome sequence from one animal caretaker was identical to the sequences obtained from lions. Chronology of the events, the viral dynamics, and the genomic data support human-to-lion transmission as the origin of infection.
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spelling pubmed-84728462021-09-28 Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses Fernández-Bellon, Hugo Rodon, Jordi Fernández-Bastit, Leira Almagro, Vanessa Padilla-Solé, Pilar Lorca-Oró, Cristina Valle, Rosa Roca, Núria Grazioli, Santina Trogu, Tiziana Bensaid, Albert Carrillo, Jorge Izquierdo-Useros, Nuria Blanco, Julià Parera, Mariona Noguera-Julián, Marc Clotet, Bonaventura Moreno, Ana Segalés, Joaquim Vergara-Alert, Júlia Viruses Article To date, no evidence supports the fact that animals play a role in the epidemiology of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus infectious disease 2019 (COVID-19). However, several animal species are naturally susceptible to SARS-CoV-2 infection. Besides pets (cats, dogs, Syrian hamsters, and ferrets) and farm animals (minks), different zoo animal species have tested positive for SARS-CoV-2 (large felids and non-human primates). After the summer of 2020, a second wave of SARS-CoV-2 infection occurred in Barcelona (Spain), reaching a peak of positive cases in November. During that period, four lions (Panthera leo) at the Barcelona Zoo and three caretakers developed respiratory signs and tested positive for the SARS-CoV-2 antigen. Lion infection was monitored for several weeks and nasal, fecal, saliva, and blood samples were taken at different time-points. SARS-CoV-2 RNA was detected in nasal samples from all studied lions and the viral RNA was detected up to two weeks after the initial viral positive test in three out of four animals. The SARS-CoV-2 genome was also detected in the feces of animals at different times. Virus isolation was successful only from respiratory samples of two lions at an early time-point. The four animals developed neutralizing antibodies after the infection that were detectable four months after the initial diagnosis. The partial SARS-CoV-2 genome sequence from one animal caretaker was identical to the sequences obtained from lions. Chronology of the events, the viral dynamics, and the genomic data support human-to-lion transmission as the origin of infection. MDPI 2021-08-25 /pmc/articles/PMC8472846/ /pubmed/34578266 http://dx.doi.org/10.3390/v13091683 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernández-Bellon, Hugo
Rodon, Jordi
Fernández-Bastit, Leira
Almagro, Vanessa
Padilla-Solé, Pilar
Lorca-Oró, Cristina
Valle, Rosa
Roca, Núria
Grazioli, Santina
Trogu, Tiziana
Bensaid, Albert
Carrillo, Jorge
Izquierdo-Useros, Nuria
Blanco, Julià
Parera, Mariona
Noguera-Julián, Marc
Clotet, Bonaventura
Moreno, Ana
Segalés, Joaquim
Vergara-Alert, Júlia
Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses
title Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses
title_full Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses
title_fullStr Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses
title_full_unstemmed Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses
title_short Monitoring Natural SARS-CoV-2 Infection in Lions (Panthera leo) at the Barcelona Zoo: Viral Dynamics and Host Responses
title_sort monitoring natural sars-cov-2 infection in lions (panthera leo) at the barcelona zoo: viral dynamics and host responses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472846/
https://www.ncbi.nlm.nih.gov/pubmed/34578266
http://dx.doi.org/10.3390/v13091683
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