Cargando…
Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster)
Siadenoviruses have been detected in wild and captive birds worldwide. Only nine siadenoviruses have been fully sequenced; however, partial sequences for 30 others, many of these from wild Australian birds, are also described. Some siadenoviruses, e.g., the turkey siadenovirus A, can cause disease;...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472863/ https://www.ncbi.nlm.nih.gov/pubmed/34578295 http://dx.doi.org/10.3390/v13091714 |
_version_ | 1784574843821228032 |
---|---|
author | Athukorala, Ajani Phalen, David N. Das, Ashutosh Helbig, Karla J. Forwood, Jade K. Sarker, Subir |
author_facet | Athukorala, Ajani Phalen, David N. Das, Ashutosh Helbig, Karla J. Forwood, Jade K. Sarker, Subir |
author_sort | Athukorala, Ajani |
collection | PubMed |
description | Siadenoviruses have been detected in wild and captive birds worldwide. Only nine siadenoviruses have been fully sequenced; however, partial sequences for 30 others, many of these from wild Australian birds, are also described. Some siadenoviruses, e.g., the turkey siadenovirus A, can cause disease; however, most cause subclinical infections. An example of a siadenovirus causing predominately subclinical infections is psittacine siadenovirus 2, proposed name psittacine siadenovirus F (PsSiAdV-F), which is enzootic in the captive breeding population of the critically endangered orange-bellied parrot (OBP, Neophema chrysogaster). Here, we have fully characterised PsSiAdV-F from an OBP. The PsSiAdV-F genome is 25,392 bp in length and contained 25 putative genes. The genome architecture of PsSiAdV-F exhibited characteristics similar to members within the genus Siadenovirus; however, the novel PsSiAdV-F genome was highly divergent, showing highest and lowest sequence similarity to skua siadenovirus A (57.1%) and psittacine siadenovirus D (31.1%), respectively. Subsequent phylogenetic analyses of the novel PsSiAdV-F genome positioned the virus into a phylogenetically distinct sub-clade with all other siadenoviruses and did not show any obvious close evolutionary relationship. Importantly, the resulted tress continually demonstrated that novel PsSiAdV-F evolved prior to all known members except the frog siadenovirus A in the evolution and possibly the ancestor of the avian siadenoviruses. To date, PsSiAdV-F has not been detected in wild parrots, so further studies screening PsSiAdV-F in wild Australian parrots and generating whole genome sequences of siadenoviruses of Australian native passerine species is recommended to fill the siadenovirus evolutionary gaps. |
format | Online Article Text |
id | pubmed-8472863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84728632021-09-28 Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) Athukorala, Ajani Phalen, David N. Das, Ashutosh Helbig, Karla J. Forwood, Jade K. Sarker, Subir Viruses Article Siadenoviruses have been detected in wild and captive birds worldwide. Only nine siadenoviruses have been fully sequenced; however, partial sequences for 30 others, many of these from wild Australian birds, are also described. Some siadenoviruses, e.g., the turkey siadenovirus A, can cause disease; however, most cause subclinical infections. An example of a siadenovirus causing predominately subclinical infections is psittacine siadenovirus 2, proposed name psittacine siadenovirus F (PsSiAdV-F), which is enzootic in the captive breeding population of the critically endangered orange-bellied parrot (OBP, Neophema chrysogaster). Here, we have fully characterised PsSiAdV-F from an OBP. The PsSiAdV-F genome is 25,392 bp in length and contained 25 putative genes. The genome architecture of PsSiAdV-F exhibited characteristics similar to members within the genus Siadenovirus; however, the novel PsSiAdV-F genome was highly divergent, showing highest and lowest sequence similarity to skua siadenovirus A (57.1%) and psittacine siadenovirus D (31.1%), respectively. Subsequent phylogenetic analyses of the novel PsSiAdV-F genome positioned the virus into a phylogenetically distinct sub-clade with all other siadenoviruses and did not show any obvious close evolutionary relationship. Importantly, the resulted tress continually demonstrated that novel PsSiAdV-F evolved prior to all known members except the frog siadenovirus A in the evolution and possibly the ancestor of the avian siadenoviruses. To date, PsSiAdV-F has not been detected in wild parrots, so further studies screening PsSiAdV-F in wild Australian parrots and generating whole genome sequences of siadenoviruses of Australian native passerine species is recommended to fill the siadenovirus evolutionary gaps. MDPI 2021-08-28 /pmc/articles/PMC8472863/ /pubmed/34578295 http://dx.doi.org/10.3390/v13091714 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Athukorala, Ajani Phalen, David N. Das, Ashutosh Helbig, Karla J. Forwood, Jade K. Sarker, Subir Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) |
title | Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) |
title_full | Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) |
title_fullStr | Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) |
title_full_unstemmed | Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) |
title_short | Genomic Characterisation of a Highly Divergent Siadenovirus (Psittacine Siadenovirus F) from the Critically Endangered Orange-Bellied Parrot (Neophema chrysogaster) |
title_sort | genomic characterisation of a highly divergent siadenovirus (psittacine siadenovirus f) from the critically endangered orange-bellied parrot (neophema chrysogaster) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472863/ https://www.ncbi.nlm.nih.gov/pubmed/34578295 http://dx.doi.org/10.3390/v13091714 |
work_keys_str_mv | AT athukoralaajani genomiccharacterisationofahighlydivergentsiadenoviruspsittacinesiadenovirusffromthecriticallyendangeredorangebelliedparrotneophemachrysogaster AT phalendavidn genomiccharacterisationofahighlydivergentsiadenoviruspsittacinesiadenovirusffromthecriticallyendangeredorangebelliedparrotneophemachrysogaster AT dasashutosh genomiccharacterisationofahighlydivergentsiadenoviruspsittacinesiadenovirusffromthecriticallyendangeredorangebelliedparrotneophemachrysogaster AT helbigkarlaj genomiccharacterisationofahighlydivergentsiadenoviruspsittacinesiadenovirusffromthecriticallyendangeredorangebelliedparrotneophemachrysogaster AT forwoodjadek genomiccharacterisationofahighlydivergentsiadenoviruspsittacinesiadenovirusffromthecriticallyendangeredorangebelliedparrotneophemachrysogaster AT sarkersubir genomiccharacterisationofahighlydivergentsiadenoviruspsittacinesiadenovirusffromthecriticallyendangeredorangebelliedparrotneophemachrysogaster |