Cargando…
Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity
Vaccination remains one of the most successful medical interventions in history, significantly decreasing morbidity and mortality associated with, or even eradicating, numerous infectious diseases. Although traditional immunization strategies have recently proven insufficient in the face of many hig...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473051/ https://www.ncbi.nlm.nih.gov/pubmed/34579201 http://dx.doi.org/10.3390/vaccines9090964 |
_version_ | 1784574891620564992 |
---|---|
author | Pilewski, Kelsey A. Kramer, Kevin J. Georgiev, Ivelin S. |
author_facet | Pilewski, Kelsey A. Kramer, Kevin J. Georgiev, Ivelin S. |
author_sort | Pilewski, Kelsey A. |
collection | PubMed |
description | Vaccination remains one of the most successful medical interventions in history, significantly decreasing morbidity and mortality associated with, or even eradicating, numerous infectious diseases. Although traditional immunization strategies have recently proven insufficient in the face of many highly mutable and emerging pathogens, modern strategies aim to rationally engineer a single antigen or cocktail of antigens to generate a focused, protective immune response. However, the effect of cocktail vaccination (simultaneous immunization with multiple immunogens) on the antibody response to each individual antigen within the combination, remains largely unstudied. To investigate whether immunization with a cocktail of diverse antigens would result in decreased antibody titer against each unique antigen in the cocktail compared to immunization with each antigen alone, we immunized mice with surface proteins from uropathogenic Escherichia coli, Mycobacterium tuberculosis, and Neisseria meningitides, and monitored the development of antigen-specific IgG antibody responses. We found that antigen-specific endpoint antibody titers were comparable across immunization groups by study conclusion (day 70). Further, we discovered that although cocktail-immunized mice initially elicited more robust antibody responses, the rate of titer development decreases significantly over time compared to single antigen-immunized mice. Investigating the basic properties that govern the development of antigen-specific antibody responses will help inform the design of future combination immunization regimens. |
format | Online Article Text |
id | pubmed-8473051 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84730512021-09-28 Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity Pilewski, Kelsey A. Kramer, Kevin J. Georgiev, Ivelin S. Vaccines (Basel) Article Vaccination remains one of the most successful medical interventions in history, significantly decreasing morbidity and mortality associated with, or even eradicating, numerous infectious diseases. Although traditional immunization strategies have recently proven insufficient in the face of many highly mutable and emerging pathogens, modern strategies aim to rationally engineer a single antigen or cocktail of antigens to generate a focused, protective immune response. However, the effect of cocktail vaccination (simultaneous immunization with multiple immunogens) on the antibody response to each individual antigen within the combination, remains largely unstudied. To investigate whether immunization with a cocktail of diverse antigens would result in decreased antibody titer against each unique antigen in the cocktail compared to immunization with each antigen alone, we immunized mice with surface proteins from uropathogenic Escherichia coli, Mycobacterium tuberculosis, and Neisseria meningitides, and monitored the development of antigen-specific IgG antibody responses. We found that antigen-specific endpoint antibody titers were comparable across immunization groups by study conclusion (day 70). Further, we discovered that although cocktail-immunized mice initially elicited more robust antibody responses, the rate of titer development decreases significantly over time compared to single antigen-immunized mice. Investigating the basic properties that govern the development of antigen-specific antibody responses will help inform the design of future combination immunization regimens. MDPI 2021-08-28 /pmc/articles/PMC8473051/ /pubmed/34579201 http://dx.doi.org/10.3390/vaccines9090964 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pilewski, Kelsey A. Kramer, Kevin J. Georgiev, Ivelin S. Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity |
title | Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity |
title_full | Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity |
title_fullStr | Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity |
title_full_unstemmed | Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity |
title_short | Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity |
title_sort | simultaneous immunization with multiple diverse immunogens alters development of antigen-specific antibody-mediated immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473051/ https://www.ncbi.nlm.nih.gov/pubmed/34579201 http://dx.doi.org/10.3390/vaccines9090964 |
work_keys_str_mv | AT pilewskikelseya simultaneousimmunizationwithmultiplediverseimmunogensaltersdevelopmentofantigenspecificantibodymediatedimmunity AT kramerkevinj simultaneousimmunizationwithmultiplediverseimmunogensaltersdevelopmentofantigenspecificantibodymediatedimmunity AT georgievivelins simultaneousimmunizationwithmultiplediverseimmunogensaltersdevelopmentofantigenspecificantibodymediatedimmunity |