Cargando…
Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella
Glycoalkaloids, secondary metabolites abundant in plants belonging to the Solanaceae family, may affect the physiology of insect pests. This paper presents original results dealing with the influence of a crude extract obtained from Solanum nigrum unripe berries and its main constituent, solasonine,...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473104/ https://www.ncbi.nlm.nih.gov/pubmed/34564621 http://dx.doi.org/10.3390/toxins13090617 |
_version_ | 1784574905038143488 |
---|---|
author | Spochacz, Marta Chowański, Szymon Szymczak-Cendlak, Monika Marciniak, Paweł Lelario, Filomena Salvia, Rosanna Nardiello, Marisa Scieuzo, Carmen Scrano, Laura Bufo, Sabino A. Adamski, Zbigniew Falabella, Patrizia |
author_facet | Spochacz, Marta Chowański, Szymon Szymczak-Cendlak, Monika Marciniak, Paweł Lelario, Filomena Salvia, Rosanna Nardiello, Marisa Scieuzo, Carmen Scrano, Laura Bufo, Sabino A. Adamski, Zbigniew Falabella, Patrizia |
author_sort | Spochacz, Marta |
collection | PubMed |
description | Glycoalkaloids, secondary metabolites abundant in plants belonging to the Solanaceae family, may affect the physiology of insect pests. This paper presents original results dealing with the influence of a crude extract obtained from Solanum nigrum unripe berries and its main constituent, solasonine, on the physiology of Galleria mellonella (Lepidoptera) that can be used as an alternative bioinsecticide. G. mellonella IV instar larvae were treated with S. nigrum extract and solasonine at different concentrations. The effects of extract and solasonine were evaluated analyzing changes in carbohydrate and amino acid composition in hemolymph by RP-HPLC and in the ultrastructure of the fat body cells by TEM. Both extract and solasonine changed the level of hemolymph metabolites and the ultrastructure of the fat body and the midgut cells. In particular, the extract increased the erythritol level in the hemolymph compared to control, enlarged the intracellular space in fat body cells, and decreased cytoplasm and lipid droplets electron density. The solasonine, tested with three concentrations, caused the decrease of cytoplasm electron density in both fat body and midgut cells. Obtained results highlighted the disturbance of the midgut and the fat body due to glycoalkaloids and the potential role of hemolymph ingredients in its detoxification. These findings suggest a possible application of glycoalkaloids as a natural insecticide in the pest control of G. mellonella larvae. |
format | Online Article Text |
id | pubmed-8473104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84731042021-09-28 Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella Spochacz, Marta Chowański, Szymon Szymczak-Cendlak, Monika Marciniak, Paweł Lelario, Filomena Salvia, Rosanna Nardiello, Marisa Scieuzo, Carmen Scrano, Laura Bufo, Sabino A. Adamski, Zbigniew Falabella, Patrizia Toxins (Basel) Article Glycoalkaloids, secondary metabolites abundant in plants belonging to the Solanaceae family, may affect the physiology of insect pests. This paper presents original results dealing with the influence of a crude extract obtained from Solanum nigrum unripe berries and its main constituent, solasonine, on the physiology of Galleria mellonella (Lepidoptera) that can be used as an alternative bioinsecticide. G. mellonella IV instar larvae were treated with S. nigrum extract and solasonine at different concentrations. The effects of extract and solasonine were evaluated analyzing changes in carbohydrate and amino acid composition in hemolymph by RP-HPLC and in the ultrastructure of the fat body cells by TEM. Both extract and solasonine changed the level of hemolymph metabolites and the ultrastructure of the fat body and the midgut cells. In particular, the extract increased the erythritol level in the hemolymph compared to control, enlarged the intracellular space in fat body cells, and decreased cytoplasm and lipid droplets electron density. The solasonine, tested with three concentrations, caused the decrease of cytoplasm electron density in both fat body and midgut cells. Obtained results highlighted the disturbance of the midgut and the fat body due to glycoalkaloids and the potential role of hemolymph ingredients in its detoxification. These findings suggest a possible application of glycoalkaloids as a natural insecticide in the pest control of G. mellonella larvae. MDPI 2021-09-01 /pmc/articles/PMC8473104/ /pubmed/34564621 http://dx.doi.org/10.3390/toxins13090617 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Spochacz, Marta Chowański, Szymon Szymczak-Cendlak, Monika Marciniak, Paweł Lelario, Filomena Salvia, Rosanna Nardiello, Marisa Scieuzo, Carmen Scrano, Laura Bufo, Sabino A. Adamski, Zbigniew Falabella, Patrizia Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella |
title | Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella |
title_full | Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella |
title_fullStr | Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella |
title_full_unstemmed | Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella |
title_short | Solanum nigrum Extract and Solasonine Affected Hemolymph Metabolites and Ultrastructure of the Fat Body and the Midgut in Galleria mellonella |
title_sort | solanum nigrum extract and solasonine affected hemolymph metabolites and ultrastructure of the fat body and the midgut in galleria mellonella |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473104/ https://www.ncbi.nlm.nih.gov/pubmed/34564621 http://dx.doi.org/10.3390/toxins13090617 |
work_keys_str_mv | AT spochaczmarta solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT chowanskiszymon solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT szymczakcendlakmonika solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT marciniakpaweł solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT lelariofilomena solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT salviarosanna solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT nardiellomarisa solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT scieuzocarmen solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT scranolaura solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT bufosabinoa solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT adamskizbigniew solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella AT falabellapatrizia solanumnigrumextractandsolasonineaffectedhemolymphmetabolitesandultrastructureofthefatbodyandthemidgutingalleriamellonella |