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Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia

Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-...

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Autores principales: Ogata, Shohei, Pereira, Juan Antonio Cristian, Jhonny, Loza Vega Ariel, Carolina, Herbas Perez Gladys, Matsuno, Keita, Orba, Yasuko, Sawa, Hirofumi, Kawamori, Fumihiko, Nonaka, Nariaki, Nakao, Ryo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473379/
https://www.ncbi.nlm.nih.gov/pubmed/34564582
http://dx.doi.org/10.3390/vetsci8090188
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author Ogata, Shohei
Pereira, Juan Antonio Cristian
Jhonny, Loza Vega Ariel
Carolina, Herbas Perez Gladys
Matsuno, Keita
Orba, Yasuko
Sawa, Hirofumi
Kawamori, Fumihiko
Nonaka, Nariaki
Nakao, Ryo
author_facet Ogata, Shohei
Pereira, Juan Antonio Cristian
Jhonny, Loza Vega Ariel
Carolina, Herbas Perez Gladys
Matsuno, Keita
Orba, Yasuko
Sawa, Hirofumi
Kawamori, Fumihiko
Nonaka, Nariaki
Nakao, Ryo
author_sort Ogata, Shohei
collection PubMed
description Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and Anaplasmataceae was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. Babesia infection was observed in nine cattle (one Babesia bovis and eight Babesia bigemina), while Anaplasmataceae infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of Anaplasma marginale and Anaplasma platys-like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies.
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spelling pubmed-84733792021-09-28 Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia Ogata, Shohei Pereira, Juan Antonio Cristian Jhonny, Loza Vega Ariel Carolina, Herbas Perez Gladys Matsuno, Keita Orba, Yasuko Sawa, Hirofumi Kawamori, Fumihiko Nonaka, Nariaki Nakao, Ryo Vet Sci Article Latin American countries produce more than a quarter of the world’s beef and are a major global supplier of livestock protein. Tick-borne diseases (TBDs) are a major constraint to the livestock industry worldwide, including in Latin America. The aim of this study was to detect and characterise tick-borne pathogens in cattle from Santa Cruz, Bolivia, where no detailed epidemiological data are available. Blood samples were collected from 104 cattle. Apicomplexan parasites were detected by nested PCR amplification of the 18S ribosomal RNA gene (rDNA), and Anaplasmataceae was screened by the PCR amplification of 16S rDNA, followed by characterisation based on the heat shock protein and citrate synthase gene sequences. Babesia infection was observed in nine cattle (one Babesia bovis and eight Babesia bigemina), while Anaplasmataceae infection was detected in thirty-two cattle. A sequencing analysis confirmed the presence of Anaplasma marginale and Anaplasma platys-like. These results provide the first molecular evidence for the four above-mentioned tick-borne pathogens in cattle in Bolivia. This information improves our understanding of the epidemiology of TBDs and will help in formulating appropriate and improved pathogen control strategies. MDPI 2021-09-07 /pmc/articles/PMC8473379/ /pubmed/34564582 http://dx.doi.org/10.3390/vetsci8090188 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ogata, Shohei
Pereira, Juan Antonio Cristian
Jhonny, Loza Vega Ariel
Carolina, Herbas Perez Gladys
Matsuno, Keita
Orba, Yasuko
Sawa, Hirofumi
Kawamori, Fumihiko
Nonaka, Nariaki
Nakao, Ryo
Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia
title Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia
title_full Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia
title_fullStr Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia
title_full_unstemmed Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia
title_short Molecular Survey of Babesia and Anaplasma Infection in Cattle in Bolivia
title_sort molecular survey of babesia and anaplasma infection in cattle in bolivia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473379/
https://www.ncbi.nlm.nih.gov/pubmed/34564582
http://dx.doi.org/10.3390/vetsci8090188
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