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CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML

Background: CircRNA has appeared as a critical molecular in the development of various cancers. However, the cellular function of circRNAs and exosomal circRNAs has not been well explored in Chronic myeloid leukemia (CML). Methods: Differentially expressed circRNAs were identified by a human circRNA...

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Autores principales: Zhong, An-Ni, Yin, Yi, Tang, Bing-Jie, Chen, Lei, Shen, Hong-Wei, Tan, Zhi-Ping, Li, Wen-Qun, He, Qun, Sun, Bao, Zhu, Yan, Xiao, Jie, Jiang, Zhi-Ping, Xu, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473700/
https://www.ncbi.nlm.nih.gov/pubmed/34588984
http://dx.doi.org/10.3389/fphar.2021.728916
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author Zhong, An-Ni
Yin, Yi
Tang, Bing-Jie
Chen, Lei
Shen, Hong-Wei
Tan, Zhi-Ping
Li, Wen-Qun
He, Qun
Sun, Bao
Zhu, Yan
Xiao, Jie
Jiang, Zhi-Ping
Xu, Ping
author_facet Zhong, An-Ni
Yin, Yi
Tang, Bing-Jie
Chen, Lei
Shen, Hong-Wei
Tan, Zhi-Ping
Li, Wen-Qun
He, Qun
Sun, Bao
Zhu, Yan
Xiao, Jie
Jiang, Zhi-Ping
Xu, Ping
author_sort Zhong, An-Ni
collection PubMed
description Background: CircRNA has appeared as a critical molecular in the development of various cancers. However, the cellular function of circRNAs and exosomal circRNAs has not been well explored in Chronic myeloid leukemia (CML). Methods: Differentially expressed circRNAs were identified by a human circRNA microarray analysis. The expression of hsa_circ_0058493 in peripheral blood mononuclear cells (PBMCs) and exosomes was verified using quantitative real-time PCR. Short hairpin RNAs against hsa_circ_0058493 were constructed to silence the expression of circ_0058493. CCK8, flow cytometry and EdU assay were performed to investigate the biological functions of circ_0058493. Results: Hsa_circ_0058493 was significantly overexpressed in the PBMCs of CML patients and high level of circ_0058493 was associated with the poor clinical efficacy of imatinib. Silencing the expression of circ_0058493 significantly inhibited the development of imatinib-resistant CML cells. miR-548b-3p was overexpressed in circ_0058493-downregulated CML cells. Bioinformatic analysis revealed that circ_0058493 might exert its regulatory function acting as a “sponge” of miR-548b-3p. Moreover, hsa_circ_0058493 was significantly enriched in the exosomes derived from imatinib-resistant CML cells. Conclusion: Hsa_circ_0058493 in PBMCs could be a promising prognostic biomarker and might provide a therapeutic target for CML treatment.
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spelling pubmed-84737002021-09-28 CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML Zhong, An-Ni Yin, Yi Tang, Bing-Jie Chen, Lei Shen, Hong-Wei Tan, Zhi-Ping Li, Wen-Qun He, Qun Sun, Bao Zhu, Yan Xiao, Jie Jiang, Zhi-Ping Xu, Ping Front Pharmacol Pharmacology Background: CircRNA has appeared as a critical molecular in the development of various cancers. However, the cellular function of circRNAs and exosomal circRNAs has not been well explored in Chronic myeloid leukemia (CML). Methods: Differentially expressed circRNAs were identified by a human circRNA microarray analysis. The expression of hsa_circ_0058493 in peripheral blood mononuclear cells (PBMCs) and exosomes was verified using quantitative real-time PCR. Short hairpin RNAs against hsa_circ_0058493 were constructed to silence the expression of circ_0058493. CCK8, flow cytometry and EdU assay were performed to investigate the biological functions of circ_0058493. Results: Hsa_circ_0058493 was significantly overexpressed in the PBMCs of CML patients and high level of circ_0058493 was associated with the poor clinical efficacy of imatinib. Silencing the expression of circ_0058493 significantly inhibited the development of imatinib-resistant CML cells. miR-548b-3p was overexpressed in circ_0058493-downregulated CML cells. Bioinformatic analysis revealed that circ_0058493 might exert its regulatory function acting as a “sponge” of miR-548b-3p. Moreover, hsa_circ_0058493 was significantly enriched in the exosomes derived from imatinib-resistant CML cells. Conclusion: Hsa_circ_0058493 in PBMCs could be a promising prognostic biomarker and might provide a therapeutic target for CML treatment. Frontiers Media S.A. 2021-09-13 /pmc/articles/PMC8473700/ /pubmed/34588984 http://dx.doi.org/10.3389/fphar.2021.728916 Text en Copyright © 2021 Zhong, Yin, Tang, Chen, Shen, Tan, Li, He, Sun, Zhu, Xiao, Jiang and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhong, An-Ni
Yin, Yi
Tang, Bing-Jie
Chen, Lei
Shen, Hong-Wei
Tan, Zhi-Ping
Li, Wen-Qun
He, Qun
Sun, Bao
Zhu, Yan
Xiao, Jie
Jiang, Zhi-Ping
Xu, Ping
CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
title CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
title_full CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
title_fullStr CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
title_full_unstemmed CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
title_short CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML
title_sort circrna microarray profiling reveals hsa_circ_0058493 as a novel biomarker for imatinib-resistant cml
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473700/
https://www.ncbi.nlm.nih.gov/pubmed/34588984
http://dx.doi.org/10.3389/fphar.2021.728916
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