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An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites
Proliferation of Plasmodium falciparum in red blood cells is the cause of malaria and is underpinned by an unconventional cell division mode, called schizogony. Contrary to model organisms, P. falciparum replicates by multiple rounds of nuclear divisions that are not interrupted by cytokinesis. Orga...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473725/ https://www.ncbi.nlm.nih.gov/pubmed/34535568 http://dx.doi.org/10.26508/lsa.202101199 |
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author | Simon, Caroline S Funaya, Charlotta Bauer, Johanna Voβ, Yannik Machado, Marta Penning, Alexander Klaschka, Darius Cyrklaff, Marek Kim, Juyeop Ganter, Markus Guizetti, Julien |
author_facet | Simon, Caroline S Funaya, Charlotta Bauer, Johanna Voβ, Yannik Machado, Marta Penning, Alexander Klaschka, Darius Cyrklaff, Marek Kim, Juyeop Ganter, Markus Guizetti, Julien |
author_sort | Simon, Caroline S |
collection | PubMed |
description | Proliferation of Plasmodium falciparum in red blood cells is the cause of malaria and is underpinned by an unconventional cell division mode, called schizogony. Contrary to model organisms, P. falciparum replicates by multiple rounds of nuclear divisions that are not interrupted by cytokinesis. Organization and dynamics of critical nuclear division factors remain poorly understood. Centriolar plaques, the centrosomes of P. falciparum, serve as microtubule organizing centers and have an acentriolar, amorphous structure. The small size of parasite nuclei has precluded detailed analysis of intranuclear microtubule organization by classical fluorescence microscopy. We apply recently developed super-resolution and time-lapse imaging protocols to describe microtubule reconfiguration during schizogony. Analysis of centrin, nuclear pore, and microtubule positioning reveals two distinct compartments of the centriolar plaque. Whereas centrin is extranuclear, we confirm by correlative light and electron tomography that microtubules are nucleated in a previously unknown and extended intranuclear compartment, which is devoid of chromatin but protein-dense. This study generates a working model for an unconventional centrosome and enables a better understanding about the diversity of eukaryotic cell division. |
format | Online Article Text |
id | pubmed-8473725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-84737252021-10-08 An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites Simon, Caroline S Funaya, Charlotta Bauer, Johanna Voβ, Yannik Machado, Marta Penning, Alexander Klaschka, Darius Cyrklaff, Marek Kim, Juyeop Ganter, Markus Guizetti, Julien Life Sci Alliance Research Articles Proliferation of Plasmodium falciparum in red blood cells is the cause of malaria and is underpinned by an unconventional cell division mode, called schizogony. Contrary to model organisms, P. falciparum replicates by multiple rounds of nuclear divisions that are not interrupted by cytokinesis. Organization and dynamics of critical nuclear division factors remain poorly understood. Centriolar plaques, the centrosomes of P. falciparum, serve as microtubule organizing centers and have an acentriolar, amorphous structure. The small size of parasite nuclei has precluded detailed analysis of intranuclear microtubule organization by classical fluorescence microscopy. We apply recently developed super-resolution and time-lapse imaging protocols to describe microtubule reconfiguration during schizogony. Analysis of centrin, nuclear pore, and microtubule positioning reveals two distinct compartments of the centriolar plaque. Whereas centrin is extranuclear, we confirm by correlative light and electron tomography that microtubules are nucleated in a previously unknown and extended intranuclear compartment, which is devoid of chromatin but protein-dense. This study generates a working model for an unconventional centrosome and enables a better understanding about the diversity of eukaryotic cell division. Life Science Alliance LLC 2021-09-17 /pmc/articles/PMC8473725/ /pubmed/34535568 http://dx.doi.org/10.26508/lsa.202101199 Text en © 2021 Simon et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Simon, Caroline S Funaya, Charlotta Bauer, Johanna Voβ, Yannik Machado, Marta Penning, Alexander Klaschka, Darius Cyrklaff, Marek Kim, Juyeop Ganter, Markus Guizetti, Julien An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
title | An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
title_full | An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
title_fullStr | An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
title_full_unstemmed | An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
title_short | An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
title_sort | extended dna-free intranuclear compartment organizes centrosome microtubules in malaria parasites |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473725/ https://www.ncbi.nlm.nih.gov/pubmed/34535568 http://dx.doi.org/10.26508/lsa.202101199 |
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