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Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection

The European wild boar (WB) (Sus scrofa) population has rapidly expanded over the years, raising public health concerns over the species reservoir of several pathogens, including Mycobacterium microti (Mm), a Mycobacterium tuberculosis complex member. In this study, we aimed to investigate the Mm na...

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Autores principales: Pigoli, Claudio, Tranquillo, Vito, Gibelli, Lucia Rita, Gaffuri, Alessandra, Alborali, Giovanni Loris, Pacciarini, Maria, Zanoni, Mariagrazia, Boniotti, Maria Beatrice, Sironi, Giuseppe, Caniatti, Mario, Grieco, Valeria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473807/
https://www.ncbi.nlm.nih.gov/pubmed/34589536
http://dx.doi.org/10.3389/fvets.2021.734919
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author Pigoli, Claudio
Tranquillo, Vito
Gibelli, Lucia Rita
Gaffuri, Alessandra
Alborali, Giovanni Loris
Pacciarini, Maria
Zanoni, Mariagrazia
Boniotti, Maria Beatrice
Sironi, Giuseppe
Caniatti, Mario
Grieco, Valeria
author_facet Pigoli, Claudio
Tranquillo, Vito
Gibelli, Lucia Rita
Gaffuri, Alessandra
Alborali, Giovanni Loris
Pacciarini, Maria
Zanoni, Mariagrazia
Boniotti, Maria Beatrice
Sironi, Giuseppe
Caniatti, Mario
Grieco, Valeria
author_sort Pigoli, Claudio
collection PubMed
description The European wild boar (WB) (Sus scrofa) population has rapidly expanded over the years, raising public health concerns over the species reservoir of several pathogens, including Mycobacterium microti (Mm), a Mycobacterium tuberculosis complex member. In this study, we aimed to investigate the Mm natural infection in WB in Lombardy and Emilia Romagna Italian regions by statistically evaluating the granulomatous lesions' histological features and Mm microbiological isolation. We analyzed 103 WB retropharyngeal and submandibular lymph nodes (LNs) for Mm identified by gyrB PCR-restriction fragment length polymorphism, and were retrospectively selected and histologically assessed. For each sample, Hematoxylin-eosin and Ziehl–Neelsen stained slides were evaluated. Considered histological variables were: the number of granulomas, size and maturational stage of granulomas, granulomas completeness within the section, number of multinucleated giant macrophages (MGMs), and acid-fast (AF) bacilli per granuloma. Furthermore, Mm microbiological results were also considered. Mm microbiological isolation was negatively influenced by granulomas maturation and positively affected by AF bacilli's presence within the section. Granuloma maturation was positively influenced by granuloma size and granuloma incompleteness and negatively affected by the number of granulomas in the section and the number of MGMs within the granuloma. The results indicate that granuloma maturation should ensures an efficient containment of Mm infection in the WB, suggesting that the intra-species transmission of the disease might be an unlikely event.
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spelling pubmed-84738072021-09-28 Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection Pigoli, Claudio Tranquillo, Vito Gibelli, Lucia Rita Gaffuri, Alessandra Alborali, Giovanni Loris Pacciarini, Maria Zanoni, Mariagrazia Boniotti, Maria Beatrice Sironi, Giuseppe Caniatti, Mario Grieco, Valeria Front Vet Sci Veterinary Science The European wild boar (WB) (Sus scrofa) population has rapidly expanded over the years, raising public health concerns over the species reservoir of several pathogens, including Mycobacterium microti (Mm), a Mycobacterium tuberculosis complex member. In this study, we aimed to investigate the Mm natural infection in WB in Lombardy and Emilia Romagna Italian regions by statistically evaluating the granulomatous lesions' histological features and Mm microbiological isolation. We analyzed 103 WB retropharyngeal and submandibular lymph nodes (LNs) for Mm identified by gyrB PCR-restriction fragment length polymorphism, and were retrospectively selected and histologically assessed. For each sample, Hematoxylin-eosin and Ziehl–Neelsen stained slides were evaluated. Considered histological variables were: the number of granulomas, size and maturational stage of granulomas, granulomas completeness within the section, number of multinucleated giant macrophages (MGMs), and acid-fast (AF) bacilli per granuloma. Furthermore, Mm microbiological results were also considered. Mm microbiological isolation was negatively influenced by granulomas maturation and positively affected by AF bacilli's presence within the section. Granuloma maturation was positively influenced by granuloma size and granuloma incompleteness and negatively affected by the number of granulomas in the section and the number of MGMs within the granuloma. The results indicate that granuloma maturation should ensures an efficient containment of Mm infection in the WB, suggesting that the intra-species transmission of the disease might be an unlikely event. Frontiers Media S.A. 2021-09-13 /pmc/articles/PMC8473807/ /pubmed/34589536 http://dx.doi.org/10.3389/fvets.2021.734919 Text en Copyright © 2021 Pigoli, Tranquillo, Gibelli, Gaffuri, Alborali, Pacciarini, Zanoni, Boniotti, Sironi, Caniatti and Grieco. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Pigoli, Claudio
Tranquillo, Vito
Gibelli, Lucia Rita
Gaffuri, Alessandra
Alborali, Giovanni Loris
Pacciarini, Maria
Zanoni, Mariagrazia
Boniotti, Maria Beatrice
Sironi, Giuseppe
Caniatti, Mario
Grieco, Valeria
Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection
title Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection
title_full Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection
title_fullStr Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection
title_full_unstemmed Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection
title_short Mycobacterium microti Infection in Wild Boar (Sus scrofa): Histopathology Analysis Suggests Containment of the Infection
title_sort mycobacterium microti infection in wild boar (sus scrofa): histopathology analysis suggests containment of the infection
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473807/
https://www.ncbi.nlm.nih.gov/pubmed/34589536
http://dx.doi.org/10.3389/fvets.2021.734919
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