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Chromosome-scale assembly of wild barley accession “OUH602”

Barley (Hordeum vulgare) was domesticated from its wild ancestral form ca. 10,000 years ago in the Fertile Crescent and is widely cultivated throughout the world, except for in tropical areas. The genome size of both cultivated barley and its conspecific wild ancestor is approximately 5 Gb. High-qua...

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Autores principales: Sato, Kazuhiro, Mascher, Martin, Himmelbach, Axel, Haberer, Georg, Spannagl, Manuel, Stein, Nils
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473966/
https://www.ncbi.nlm.nih.gov/pubmed/34568912
http://dx.doi.org/10.1093/g3journal/jkab244
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author Sato, Kazuhiro
Mascher, Martin
Himmelbach, Axel
Haberer, Georg
Spannagl, Manuel
Stein, Nils
author_facet Sato, Kazuhiro
Mascher, Martin
Himmelbach, Axel
Haberer, Georg
Spannagl, Manuel
Stein, Nils
author_sort Sato, Kazuhiro
collection PubMed
description Barley (Hordeum vulgare) was domesticated from its wild ancestral form ca. 10,000 years ago in the Fertile Crescent and is widely cultivated throughout the world, except for in tropical areas. The genome size of both cultivated barley and its conspecific wild ancestor is approximately 5 Gb. High-quality chromosome-level assemblies of 19 cultivated and one wild barley genotype were recently established by pan-genome analysis. Here, we release another equivalent short-read assembly of the wild barley accession “OUH602.” A series of genetic and genomic resources were developed for this genotype in prior studies. Our assembly contains more than 4.4 Gb of sequence, with a scaffold N50 value of over 10 Mb. The haplotype shows high collinearity with the most recently updated barley reference genome, “Morex” V3, with some inversions. Gene projections based on “Morex” gene models revealed 46,807 protein-coding sequences and 43,375 protein-coding genes. Alignments to publicly available sequences of bacterial artificial chromosome (BAC) clones of “OUH602” confirm the high accuracy of the assembly. Since more loci of interest have been identified in “OUH602,” the release of this assembly, with detailed genomic information, should accelerate gene identification and the utilization of this key wild barley accession.
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spelling pubmed-84739662021-09-27 Chromosome-scale assembly of wild barley accession “OUH602” Sato, Kazuhiro Mascher, Martin Himmelbach, Axel Haberer, Georg Spannagl, Manuel Stein, Nils G3 (Bethesda) Genome Report Barley (Hordeum vulgare) was domesticated from its wild ancestral form ca. 10,000 years ago in the Fertile Crescent and is widely cultivated throughout the world, except for in tropical areas. The genome size of both cultivated barley and its conspecific wild ancestor is approximately 5 Gb. High-quality chromosome-level assemblies of 19 cultivated and one wild barley genotype were recently established by pan-genome analysis. Here, we release another equivalent short-read assembly of the wild barley accession “OUH602.” A series of genetic and genomic resources were developed for this genotype in prior studies. Our assembly contains more than 4.4 Gb of sequence, with a scaffold N50 value of over 10 Mb. The haplotype shows high collinearity with the most recently updated barley reference genome, “Morex” V3, with some inversions. Gene projections based on “Morex” gene models revealed 46,807 protein-coding sequences and 43,375 protein-coding genes. Alignments to publicly available sequences of bacterial artificial chromosome (BAC) clones of “OUH602” confirm the high accuracy of the assembly. Since more loci of interest have been identified in “OUH602,” the release of this assembly, with detailed genomic information, should accelerate gene identification and the utilization of this key wild barley accession. Oxford University Press 2021-07-13 /pmc/articles/PMC8473966/ /pubmed/34568912 http://dx.doi.org/10.1093/g3journal/jkab244 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Report
Sato, Kazuhiro
Mascher, Martin
Himmelbach, Axel
Haberer, Georg
Spannagl, Manuel
Stein, Nils
Chromosome-scale assembly of wild barley accession “OUH602”
title Chromosome-scale assembly of wild barley accession “OUH602”
title_full Chromosome-scale assembly of wild barley accession “OUH602”
title_fullStr Chromosome-scale assembly of wild barley accession “OUH602”
title_full_unstemmed Chromosome-scale assembly of wild barley accession “OUH602”
title_short Chromosome-scale assembly of wild barley accession “OUH602”
title_sort chromosome-scale assembly of wild barley accession “ouh602”
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473966/
https://www.ncbi.nlm.nih.gov/pubmed/34568912
http://dx.doi.org/10.1093/g3journal/jkab244
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