Cargando…

A highly multiplexed quantitative phosphosite assay for biology and preclinical studies

Reliable methods to quantify dynamic signaling changes across diverse pathways are needed to better understand the effects of disease and drug treatment in cells and tissues but are presently lacking. Here, we present SigPath, a targeted mass spectrometry (MS) assay that measures 284 phosphosites in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Keshishian, Hasmik, McDonald, E Robert, Mundt, Filip, Melanson, Randy, Krug, Karsten, Porter, Dale A, Wallace, Luke, Forestier, Dominique, Rabasha, Bokang, Marlow, Sara E, Jane‐Valbuena, Judit, Todres, Ellen, Specht, Harrison, Robinson, Margaret Lea, Jean Beltran, Pierre M, Babur, Ozgun, Olive, Meagan E, Golji, Javad, Kuhn, Eric, Burgess, Michael, MacMullan, Melanie A, Rejtar, Tomas, Wang, Karen, Mani, DR, Satpathy, Shankha, Gillette, Michael A, Sellers, William R, Carr, Steven A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474009/ https://www.ncbi.nlm.nih.gov/pubmed/34569154 http://dx.doi.org/10.15252/msb.202010156

Ejemplares similares

A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
por: Wu, Chi-Hong, et al.
Publicado: (2022)

Phosphosite Mapping of HIP-55 Protein in Mammalian Cells
por: Liu, Ning, et al.
Publicado: (2014)

Mining recent brain proteomic databases for ion channel phosphosite nuggets
por: Cerda, Oscar, et al.
Publicado: (2011)

SimPhospho: a software tool enabling confident phosphosite assignment
por: Suni, Veronika, et al.
Publicado: (2018)

PHOSIDA (phosphorylation site database): management, structural and evolutionary investigation, and prediction of phosphosites
por: Gnad, Florian, et al.
Publicado: (2007)

Phosphosite-dependent presentation of dual phosphorylated peptides by MHC class I molecules
por: Zhao, Yingze, et al.
Publicado: (2022)

Unambiguous Phosphosite Localization using Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)
por: Frese, Christian K., et al.
Publicado: (2013)

Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture
por: Vega, Andrea, et al.
Publicado: (2021)

Phosphosite Scanning reveals a complex phosphorylation code underlying CDK-dependent activation of Hcm1
por: Conti, Michelle M., et al.
Publicado: (2023)

Where Do Phosphosites Come from and Where Do They Go after Gene Duplication?
por: Diss, Guillaume, et al.
Publicado: (2012)

LymPHOS 2.0: an update of a phosphosite database of primary human T cells
por: Nguyen, Tien Dung, et al.
Publicado: (2015)

In situ visualization of opioid and cannabinoid drug effects using phosphosite-specific GPCR antibodies
por: Fritzwanker, Sebastian, et al.
Publicado: (2023)

A basic phosphoproteomic-DIA workflow integrating precise quantification of phosphosites in systems biology
por: Di, Yi, et al.
Publicado: (2023)

Coevolving MAPK and PID phosphosites indicate an ancient environmental control of PIN auxin transporters in land plants
por: Dory, Magdalena, et al.
Publicado: (2017)

New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D(2) receptor regulation and signaling
por: Mann, Anika, et al.
Publicado: (2021)

Separase Phosphosite Mutation Leads to Genome Instability and Primordial Germ Cell Depletion during Oogenesis
por: Xu, Juan, et al.
Publicado: (2011)

Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues
por: Gengenbacher, Anina, et al.
Publicado: (2019)

Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit
por: Abbasi, Marjan, et al.
Publicado: (2022)

Dissecting signaling regulators driving AXL-mediated bypass resistance and associated phenotypes by phosphosite perturbations
por: Creixell, Marc, et al.
Publicado: (2023)

The meniscus, calcification and osteoarthritis: a pathologic team
por: MacMullan, Paul A, et al.
Publicado: (2010)

Automating UbiFast for High-throughput and Multiplexed Ubiquitin Enrichment
por: Rivera, Keith D., et al.
Publicado: (2021)

A Phosphosite Mutant Approach on LRRK2 Links Phosphorylation and Dephosphorylation to Protective and Deleterious Markers, Respectively
por: Marchand, Antoine, et al.
Publicado: (2022)

Accurate, high-coverage assignment of in vivo protein kinases to phosphosites from in vitro phosphoproteomic specificity data
por: Invergo, Brandon M.
Publicado: (2022)

Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance
por: Hunter, Jill E., et al.
Publicado: (2022)

14-3-3 proteins inactivate DAPK2 by promoting its dimerization and protecting key regulatory phosphosites
por: Horvath, Matej, et al.
Publicado: (2021)

Phosphorylation of human phospholipase A1 DDHD1 at newly identified phosphosites affects its subcellular localization
por: Matsumoto, Naoki, et al.
Publicado: (2021)

Improving identification of symptomatic cancer at primary care clinics: A predictive modeling analysis in Botswana
por: Molebatsi, Kesaobaka, et al.
Publicado: (2022)

Rapid and deep-scale ubiquitylation profiling for biology and translational research
por: Udeshi, Namrata D., et al.
Publicado: (2020)

Quantitative Proteomics and Metabolomics Reveal Biomarkers of Disease as Potential Immunotherapy Targets and Indicators of Therapeutic Efficacy
por: MacMullan, Melanie A., et al.
Publicado: (2019)

Leishmania donovani 90 kD Heat Shock Protein – Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity
por: Hombach-Barrigah, Antje, et al.
Publicado: (2019)

Agonist-induced phosphorylation bar code and differential post-activation signaling of the delta opioid receptor revealed by phosphosite-specific antibodies
por: Mann, Anika, et al.
Publicado: (2020)

Phenotypical Characterization of the Nuclear Egress of Recombinant Cytomegaloviruses Reveals Defective Replication upon ORF-UL50 Deletion but Not pUL50 Phosphosite Mutation
por: Häge, Sigrun, et al.
Publicado: (2021)

Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
por: MacMullan, Melanie A., et al.
Publicado: (2021)

Rapid Response to Cytokine Storm Inhibition Using Anakinra in a Patient With COVID-19 Myocarditis
por: Trpkov, Cvetan, et al.
Publicado: (2020)

Kinds of well-being: A conceptual framework that provides direction for caring
por: Galvin, Kathleen T., et al.
Publicado: (2011)

“Dwelling-mobility”: An existential theory of well-being
por: Todres, Les, et al.
Publicado: (2010)

Dignity as honour-wound: an experiential and relational view
por: Galvin, Kathleen, et al.
Publicado: (2015)

The SARS-CoV-2 RNA–protein interactome in infected human cells
por: Schmidt, Nora, et al.
Publicado: (2020)

Algorithms for effective querying of compound graph-based pathway databases
por: Dogrusoz, Ugur, et al.
Publicado: (2009)

Workflow enabling deepscale immunopeptidome, proteome, ubiquitylome, phosphoproteome, and acetylome analyses of sample-limited tissues
por: Abelin, Jennifer G., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_e0c9l3b76oatvivn92ekl3b29t