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DNA methylation and exposure to violence among African American young adult males

Exposure to violence (ETV) has been linked to epigenomics mechanisms such as DNA methylation (DNAm). We used epigenetic profiling of blood collected from 32 African American young adult males who lived in Washington DC to determine if changes in DNAm at CpG sites affiliated with nervous and immune s...

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Autores principales: Saadatmand, Forough, Gurdziel, Katherine, Jackson, Latifa, Kwabi-Addo, Bernard, Ruden, Douglas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474503/
https://www.ncbi.nlm.nih.gov/pubmed/34589758
http://dx.doi.org/10.1016/j.bbih.2021.100247
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author Saadatmand, Forough
Gurdziel, Katherine
Jackson, Latifa
Kwabi-Addo, Bernard
Ruden, Douglas M.
author_facet Saadatmand, Forough
Gurdziel, Katherine
Jackson, Latifa
Kwabi-Addo, Bernard
Ruden, Douglas M.
author_sort Saadatmand, Forough
collection PubMed
description Exposure to violence (ETV) has been linked to epigenomics mechanisms such as DNA methylation (DNAm). We used epigenetic profiling of blood collected from 32 African American young adult males who lived in Washington DC to determine if changes in DNAm at CpG sites affiliated with nervous and immune system were associated with exposure to violence. Pathway analysis of differentially methylated regions comparing high and low ETV groups revealed an enrichment of gene sets annotated to nervous system and immune ontologies. Many of these genes are known to interact with each other which suggests DNAm alters gene function in the nervous and immune system in response to ETV. Using data from a unique age group, young African American adult males, we provide evidence that lifetime ETV could impact DNA methylation in genes impacted at Central Nervous System and Immune Function sites. METHOD: Methylation analysis was performed on DNA collected from the blood of participants classified with either high or low lifetime ETV. Illumina®MethylationEPIC Beadchips (~850k CpG sites) were processed on the iScan System to examine whole-genome methylation differences. Differentially methylated CpG-sites between high (n ​= ​19) and low (n ​= ​13) groups were identified using linear regression with violence and substance abuse as model covariates. Gene ontology analysis was used to identify enrichment categories from probes annotated to the nearest gene. RESULTS: A total of 595 probes (279 hypermethylated; 316 hypomethylated) annotated to 383 genes were considered differentially methylated in association with ETV. Males with high ETV showed elevated methylation in several signaling pathways but were most impacted at Central Nervous System and Immune Function affiliated sites. Eight candidate genes were identified that play important biological roles in stress response to violence with HDAC4 (10%), NR4A3 (11%), NR4A2 (12%), DSCAML1(12%), and ELAVL3 (13%) exhibiting higher levels in the low ETV group and DLGAP1 (10%), SHANK2 (10%), and NRG1(11%) having increased methylation in the high ETV group. These findings suggest that individuals subjected to high ETV may be at risk for poor health outcomes that have not been reported previously.
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spelling pubmed-84745032021-09-28 DNA methylation and exposure to violence among African American young adult males Saadatmand, Forough Gurdziel, Katherine Jackson, Latifa Kwabi-Addo, Bernard Ruden, Douglas M. Brain Behav Immun Health Full Length Article Exposure to violence (ETV) has been linked to epigenomics mechanisms such as DNA methylation (DNAm). We used epigenetic profiling of blood collected from 32 African American young adult males who lived in Washington DC to determine if changes in DNAm at CpG sites affiliated with nervous and immune system were associated with exposure to violence. Pathway analysis of differentially methylated regions comparing high and low ETV groups revealed an enrichment of gene sets annotated to nervous system and immune ontologies. Many of these genes are known to interact with each other which suggests DNAm alters gene function in the nervous and immune system in response to ETV. Using data from a unique age group, young African American adult males, we provide evidence that lifetime ETV could impact DNA methylation in genes impacted at Central Nervous System and Immune Function sites. METHOD: Methylation analysis was performed on DNA collected from the blood of participants classified with either high or low lifetime ETV. Illumina®MethylationEPIC Beadchips (~850k CpG sites) were processed on the iScan System to examine whole-genome methylation differences. Differentially methylated CpG-sites between high (n ​= ​19) and low (n ​= ​13) groups were identified using linear regression with violence and substance abuse as model covariates. Gene ontology analysis was used to identify enrichment categories from probes annotated to the nearest gene. RESULTS: A total of 595 probes (279 hypermethylated; 316 hypomethylated) annotated to 383 genes were considered differentially methylated in association with ETV. Males with high ETV showed elevated methylation in several signaling pathways but were most impacted at Central Nervous System and Immune Function affiliated sites. Eight candidate genes were identified that play important biological roles in stress response to violence with HDAC4 (10%), NR4A3 (11%), NR4A2 (12%), DSCAML1(12%), and ELAVL3 (13%) exhibiting higher levels in the low ETV group and DLGAP1 (10%), SHANK2 (10%), and NRG1(11%) having increased methylation in the high ETV group. These findings suggest that individuals subjected to high ETV may be at risk for poor health outcomes that have not been reported previously. Elsevier 2021-03-29 /pmc/articles/PMC8474503/ /pubmed/34589758 http://dx.doi.org/10.1016/j.bbih.2021.100247 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Saadatmand, Forough
Gurdziel, Katherine
Jackson, Latifa
Kwabi-Addo, Bernard
Ruden, Douglas M.
DNA methylation and exposure to violence among African American young adult males
title DNA methylation and exposure to violence among African American young adult males
title_full DNA methylation and exposure to violence among African American young adult males
title_fullStr DNA methylation and exposure to violence among African American young adult males
title_full_unstemmed DNA methylation and exposure to violence among African American young adult males
title_short DNA methylation and exposure to violence among African American young adult males
title_sort dna methylation and exposure to violence among african american young adult males
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474503/
https://www.ncbi.nlm.nih.gov/pubmed/34589758
http://dx.doi.org/10.1016/j.bbih.2021.100247
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