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Metal Ion Fluxes Controlling Amphibian Fertilization
Mammalian oocytes undergo major changes in zinc content and localization in order to be fertilized, the most striking being the rapid exocytosis of over ten billion zinc ions, known as zinc sparks. Here we report that fertilization of amphibian Xenopus laevis eggs also initiates a zinc spark that pr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475775/ https://www.ncbi.nlm.nih.gov/pubmed/34155376 http://dx.doi.org/10.1038/s41557-021-00705-2 |
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author | Seeler, John F. Sharma, Ajay Zaluzec, Nestor J. Bleher, Reiner Lai, Barry Schultz, Emma G. Hoffman, Brian M. LaBonne, Carole Woodruff, Teresa K. O’Halloran, Thomas V. |
author_facet | Seeler, John F. Sharma, Ajay Zaluzec, Nestor J. Bleher, Reiner Lai, Barry Schultz, Emma G. Hoffman, Brian M. LaBonne, Carole Woodruff, Teresa K. O’Halloran, Thomas V. |
author_sort | Seeler, John F. |
collection | PubMed |
description | Mammalian oocytes undergo major changes in zinc content and localization in order to be fertilized, the most striking being the rapid exocytosis of over ten billion zinc ions, known as zinc sparks. Here we report that fertilization of amphibian Xenopus laevis eggs also initiates a zinc spark that progresses across the cell surface in coordination with dynamic calcium waves. This zinc exocytosis is accompanied by a newly recognized loss of intracellular manganese: synchrotron-based X-ray fluorescence and analytical electron microscopy reveal that zinc and manganese are sequestered in a system of cortical granules that are abundant at the animal pole. Through Electron-Nuclear Double-Resonance (ENDOR) studies, we rule out Mn(2+) complexation with phosphate or nitrogenous ligands in intact eggs but the data are consistent with a carboxylate coordination environment. Our observations suggest that zinc and manganese fluxes are a conserved feature of fertilization in vertebrates and that they function as part of a physiological block to polyspermy. |
format | Online Article Text |
id | pubmed-8475775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-84757752021-12-21 Metal Ion Fluxes Controlling Amphibian Fertilization Seeler, John F. Sharma, Ajay Zaluzec, Nestor J. Bleher, Reiner Lai, Barry Schultz, Emma G. Hoffman, Brian M. LaBonne, Carole Woodruff, Teresa K. O’Halloran, Thomas V. Nat Chem Article Mammalian oocytes undergo major changes in zinc content and localization in order to be fertilized, the most striking being the rapid exocytosis of over ten billion zinc ions, known as zinc sparks. Here we report that fertilization of amphibian Xenopus laevis eggs also initiates a zinc spark that progresses across the cell surface in coordination with dynamic calcium waves. This zinc exocytosis is accompanied by a newly recognized loss of intracellular manganese: synchrotron-based X-ray fluorescence and analytical electron microscopy reveal that zinc and manganese are sequestered in a system of cortical granules that are abundant at the animal pole. Through Electron-Nuclear Double-Resonance (ENDOR) studies, we rule out Mn(2+) complexation with phosphate or nitrogenous ligands in intact eggs but the data are consistent with a carboxylate coordination environment. Our observations suggest that zinc and manganese fluxes are a conserved feature of fertilization in vertebrates and that they function as part of a physiological block to polyspermy. 2021-06-21 2021-07 /pmc/articles/PMC8475775/ /pubmed/34155376 http://dx.doi.org/10.1038/s41557-021-00705-2 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Seeler, John F. Sharma, Ajay Zaluzec, Nestor J. Bleher, Reiner Lai, Barry Schultz, Emma G. Hoffman, Brian M. LaBonne, Carole Woodruff, Teresa K. O’Halloran, Thomas V. Metal Ion Fluxes Controlling Amphibian Fertilization |
title | Metal Ion Fluxes Controlling Amphibian Fertilization |
title_full | Metal Ion Fluxes Controlling Amphibian Fertilization |
title_fullStr | Metal Ion Fluxes Controlling Amphibian Fertilization |
title_full_unstemmed | Metal Ion Fluxes Controlling Amphibian Fertilization |
title_short | Metal Ion Fluxes Controlling Amphibian Fertilization |
title_sort | metal ion fluxes controlling amphibian fertilization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475775/ https://www.ncbi.nlm.nih.gov/pubmed/34155376 http://dx.doi.org/10.1038/s41557-021-00705-2 |
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