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Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients

The emergence of SARS-CoV-2, responsible for coronavirus disease-2019 (COVID-19), has become a major global health problem. The molecular testing is the accepted assay in SARS-CoV-2 detection. However, there are several reasons for low sensitivity by RNA detection, causing challenges in SARS-CoV-2 d...

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Autores principales: Rostamzadeh, Davood, Mortezagholi, Sahar, Alinejad, Maedeh, Jooya, Seyed Reza, Eskandarian, Maryam, Metvaei, Alireza, Vafaei, Sedigheh, Aboulghasemi, Hasan, Younesi, Vahid, Shabani, Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475848/
https://www.ncbi.nlm.nih.gov/pubmed/34564742
http://dx.doi.org/10.1007/s00430-021-00721-6
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author Rostamzadeh, Davood
Mortezagholi, Sahar
Alinejad, Maedeh
Jooya, Seyed Reza
Eskandarian, Maryam
Metvaei, Alireza
Vafaei, Sedigheh
Aboulghasemi, Hasan
Younesi, Vahid
Shabani, Mahdi
author_facet Rostamzadeh, Davood
Mortezagholi, Sahar
Alinejad, Maedeh
Jooya, Seyed Reza
Eskandarian, Maryam
Metvaei, Alireza
Vafaei, Sedigheh
Aboulghasemi, Hasan
Younesi, Vahid
Shabani, Mahdi
author_sort Rostamzadeh, Davood
collection PubMed
description The emergence of SARS-CoV-2, responsible for coronavirus disease-2019 (COVID-19), has become a major global health problem. The molecular testing is the accepted assay in SARS-CoV-2 detection. However, there are several reasons for low sensitivity by RNA detection, causing challenges in SARS-CoV-2 diagnosis. In this study, we aimed to investigate serological patterns of SARS-CoV-2 specific IgM, and IgG in 111 hospitalized, and 34 recovered COVID-19 patients and 311 prepandemic normal serum specimens by ELISA. The validity of the ELISA kits was evaluated using samples from normal and recovered cases. This showed that 98.1%, and 98.4% of prepandemic normal samples were negative for anti-SARS-CoV-2 IgM, and IgG, respectively. Assessment of 34 COVID-19 confirmed recovered patients showed a test sensitivity of 76.5%, and 94.1% for IgM, and IgG, respectively. In COVID-19 hospitalized patients, 42.3%, and 51.4% were positive for IgM and IgG, respectively. Viral RNA was not detectable in 43.3% of the hospitalized patients. Interestingly, combined molecular and serological testing improved the sensitivity of COVID-19 diagnosis to 79.6%. Using PCR with combined IgM/IgG results augmented the patient diagnosis sensitivity to 65.3% and 87.2% in ≤ 7 days, and > 7 days intervals, respectively. Overall, serological tests in combination with PCR can improve the sensitivity of COVID-19 diagnosis.
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spelling pubmed-84758482021-09-28 Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients Rostamzadeh, Davood Mortezagholi, Sahar Alinejad, Maedeh Jooya, Seyed Reza Eskandarian, Maryam Metvaei, Alireza Vafaei, Sedigheh Aboulghasemi, Hasan Younesi, Vahid Shabani, Mahdi Med Microbiol Immunol Original Investigation The emergence of SARS-CoV-2, responsible for coronavirus disease-2019 (COVID-19), has become a major global health problem. The molecular testing is the accepted assay in SARS-CoV-2 detection. However, there are several reasons for low sensitivity by RNA detection, causing challenges in SARS-CoV-2 diagnosis. In this study, we aimed to investigate serological patterns of SARS-CoV-2 specific IgM, and IgG in 111 hospitalized, and 34 recovered COVID-19 patients and 311 prepandemic normal serum specimens by ELISA. The validity of the ELISA kits was evaluated using samples from normal and recovered cases. This showed that 98.1%, and 98.4% of prepandemic normal samples were negative for anti-SARS-CoV-2 IgM, and IgG, respectively. Assessment of 34 COVID-19 confirmed recovered patients showed a test sensitivity of 76.5%, and 94.1% for IgM, and IgG, respectively. In COVID-19 hospitalized patients, 42.3%, and 51.4% were positive for IgM and IgG, respectively. Viral RNA was not detectable in 43.3% of the hospitalized patients. Interestingly, combined molecular and serological testing improved the sensitivity of COVID-19 diagnosis to 79.6%. Using PCR with combined IgM/IgG results augmented the patient diagnosis sensitivity to 65.3% and 87.2% in ≤ 7 days, and > 7 days intervals, respectively. Overall, serological tests in combination with PCR can improve the sensitivity of COVID-19 diagnosis. Springer Berlin Heidelberg 2021-09-25 2021 /pmc/articles/PMC8475848/ /pubmed/34564742 http://dx.doi.org/10.1007/s00430-021-00721-6 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Investigation
Rostamzadeh, Davood
Mortezagholi, Sahar
Alinejad, Maedeh
Jooya, Seyed Reza
Eskandarian, Maryam
Metvaei, Alireza
Vafaei, Sedigheh
Aboulghasemi, Hasan
Younesi, Vahid
Shabani, Mahdi
Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients
title Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients
title_full Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients
title_fullStr Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients
title_full_unstemmed Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients
title_short Serological assay for anti-SARS-CoV-2 antibodies improves sensitivity of diagnosis of COVID-19 patients
title_sort serological assay for anti-sars-cov-2 antibodies improves sensitivity of diagnosis of covid-19 patients
topic Original Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475848/
https://www.ncbi.nlm.nih.gov/pubmed/34564742
http://dx.doi.org/10.1007/s00430-021-00721-6
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