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Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males
PURPOSE: Whole-body fat oxidation during exercise can be measured non-invasively during athlete profiling. Gaps in understanding exist in the relationships between fat oxidation during incremental fasted exercise and skeletal muscle parameters, endurance performance, and fat oxidation during prolong...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475903/ https://www.ncbi.nlm.nih.gov/pubmed/34562114 http://dx.doi.org/10.1007/s00421-021-04820-3 |
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author | Maunder, Ed Plews, Daniel J. Wallis, Gareth A. Brick, Matthew J. Leigh, Warren B. Chang, Wee-Leong Stewart, Tom Watkins, Casey M. Kilding, Andrew E. |
author_facet | Maunder, Ed Plews, Daniel J. Wallis, Gareth A. Brick, Matthew J. Leigh, Warren B. Chang, Wee-Leong Stewart, Tom Watkins, Casey M. Kilding, Andrew E. |
author_sort | Maunder, Ed |
collection | PubMed |
description | PURPOSE: Whole-body fat oxidation during exercise can be measured non-invasively during athlete profiling. Gaps in understanding exist in the relationships between fat oxidation during incremental fasted exercise and skeletal muscle parameters, endurance performance, and fat oxidation during prolonged fed-state exercise. METHODS: Seventeen endurance-trained males underwent a (i) fasted, incremental cycling test to assess peak whole-body fat oxidation (PFO), (ii) resting vastus lateralis microbiopsy, and (iii) 30-min maximal-effort cycling time-trial preceded by 2-h of fed-state moderate-intensity cycling to assess endurance performance and fed-state metabolism on separate occasions within one week. RESULTS: PFO (0.58 ± 0.28 g(.)min(−1)) was associated with vastus lateralis citrate synthase activity (69.2 ± 26.0 μmol(.)min(−1.)g(−1) muscle protein, r = 0.84, 95% CI 0.58, 0.95, P < 0.001), CD36 abundance (16.8 ± 12.6 μg(.)g(−1) muscle protein, r(s) = 0.68, 95% CI 0.31, 1.10, P = 0.01), pre-loaded 30-min time-trial performance (251 ± 51 W, r = 0.76, 95% CI 0.40, 0.91, P = 0.001; 3.2 ± 0.6 W(.)kg(−1), r = 0.62, 95% CI 0.16, 0.86, P = 0.01), and fat oxidation during prolonged fed-state cycling (r = 0.83, 95% CI 0.57, 0.94, P < 0.001). Addition of PFO to a traditional model of endurance (peak oxygen uptake, power at 4 mmol(.)L(−1) blood lactate concentration, and gross efficiency) explained an additional ~ 2.6% of variation in 30-min time-trial performance (adjusted R(2) = 0.903 vs. 0.877). CONCLUSION: These associations suggest non-invasive measures of whole-body fat oxidation during exercise may be useful in the physiological profiling of endurance athletes. |
format | Online Article Text |
id | pubmed-8475903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-84759032021-09-28 Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males Maunder, Ed Plews, Daniel J. Wallis, Gareth A. Brick, Matthew J. Leigh, Warren B. Chang, Wee-Leong Stewart, Tom Watkins, Casey M. Kilding, Andrew E. Eur J Appl Physiol Original Article PURPOSE: Whole-body fat oxidation during exercise can be measured non-invasively during athlete profiling. Gaps in understanding exist in the relationships between fat oxidation during incremental fasted exercise and skeletal muscle parameters, endurance performance, and fat oxidation during prolonged fed-state exercise. METHODS: Seventeen endurance-trained males underwent a (i) fasted, incremental cycling test to assess peak whole-body fat oxidation (PFO), (ii) resting vastus lateralis microbiopsy, and (iii) 30-min maximal-effort cycling time-trial preceded by 2-h of fed-state moderate-intensity cycling to assess endurance performance and fed-state metabolism on separate occasions within one week. RESULTS: PFO (0.58 ± 0.28 g(.)min(−1)) was associated with vastus lateralis citrate synthase activity (69.2 ± 26.0 μmol(.)min(−1.)g(−1) muscle protein, r = 0.84, 95% CI 0.58, 0.95, P < 0.001), CD36 abundance (16.8 ± 12.6 μg(.)g(−1) muscle protein, r(s) = 0.68, 95% CI 0.31, 1.10, P = 0.01), pre-loaded 30-min time-trial performance (251 ± 51 W, r = 0.76, 95% CI 0.40, 0.91, P = 0.001; 3.2 ± 0.6 W(.)kg(−1), r = 0.62, 95% CI 0.16, 0.86, P = 0.01), and fat oxidation during prolonged fed-state cycling (r = 0.83, 95% CI 0.57, 0.94, P < 0.001). Addition of PFO to a traditional model of endurance (peak oxygen uptake, power at 4 mmol(.)L(−1) blood lactate concentration, and gross efficiency) explained an additional ~ 2.6% of variation in 30-min time-trial performance (adjusted R(2) = 0.903 vs. 0.877). CONCLUSION: These associations suggest non-invasive measures of whole-body fat oxidation during exercise may be useful in the physiological profiling of endurance athletes. Springer Berlin Heidelberg 2021-09-25 2022 /pmc/articles/PMC8475903/ /pubmed/34562114 http://dx.doi.org/10.1007/s00421-021-04820-3 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Maunder, Ed Plews, Daniel J. Wallis, Gareth A. Brick, Matthew J. Leigh, Warren B. Chang, Wee-Leong Stewart, Tom Watkins, Casey M. Kilding, Andrew E. Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
title | Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
title_full | Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
title_fullStr | Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
title_full_unstemmed | Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
title_short | Peak fat oxidation is positively associated with vastus lateralis CD36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
title_sort | peak fat oxidation is positively associated with vastus lateralis cd36 content, fed-state exercise fat oxidation, and endurance performance in trained males |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475903/ https://www.ncbi.nlm.nih.gov/pubmed/34562114 http://dx.doi.org/10.1007/s00421-021-04820-3 |
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