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Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

In this report, we have developed a simple approach using single-detector fluorescence autocorrelation spectroscopy (FCS) to investigate the Förster resonance energy transfer (FRET) of genetically encoded, freely diffusing crTC2.1 (mTurquoise2.1–linker–mCitrine) at the single molecule level. We hypo...

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Detalles Bibliográficos
Autores principales:	Kay, Taryn M., Aplin, Cody P., Simonet, Rowan, Beenken, Julie, Miller, Robert C., Libal, Christin, Boersma, Arnold J., Sheets, Erin D., Heikal, Ahmed A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Molecular Biosciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476790/ https://www.ncbi.nlm.nih.gov/pubmed/34595208 http://dx.doi.org/10.3389/fmolb.2021.730394

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