Cargando…

Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging

Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes...

Descripción completa

Detalles Bibliográficos
Autores principales: Jing, Yingying, Zhang, Chenshuang, Yu, Bin, Lin, Danying, Qu, Junle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476955/
https://www.ncbi.nlm.nih.gov/pubmed/34595156
http://dx.doi.org/10.3389/fchem.2021.746900
_version_ 1784575735564861440
author Jing, Yingying
Zhang, Chenshuang
Yu, Bin
Lin, Danying
Qu, Junle
author_facet Jing, Yingying
Zhang, Chenshuang
Yu, Bin
Lin, Danying
Qu, Junle
author_sort Jing, Yingying
collection PubMed
description Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to in vivo imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of in vivo super resolution imaging.
format Online
Article
Text
id pubmed-8476955
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84769552021-09-29 Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging Jing, Yingying Zhang, Chenshuang Yu, Bin Lin, Danying Qu, Junle Front Chem Chemistry Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to in vivo imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of in vivo super resolution imaging. Frontiers Media S.A. 2021-09-14 /pmc/articles/PMC8476955/ /pubmed/34595156 http://dx.doi.org/10.3389/fchem.2021.746900 Text en Copyright © 2021 Jing, Zhang, Yu, Lin and Qu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Jing, Yingying
Zhang, Chenshuang
Yu, Bin
Lin, Danying
Qu, Junle
Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_full Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_fullStr Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_full_unstemmed Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_short Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_sort super-resolution microscopy: shedding new light on in vivo imaging
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476955/
https://www.ncbi.nlm.nih.gov/pubmed/34595156
http://dx.doi.org/10.3389/fchem.2021.746900
work_keys_str_mv AT jingyingying superresolutionmicroscopysheddingnewlightoninvivoimaging
AT zhangchenshuang superresolutionmicroscopysheddingnewlightoninvivoimaging
AT yubin superresolutionmicroscopysheddingnewlightoninvivoimaging
AT lindanying superresolutionmicroscopysheddingnewlightoninvivoimaging
AT qujunle superresolutionmicroscopysheddingnewlightoninvivoimaging