Cargando…

Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants

Phosphorus (P) is one of the essential macronutrients, whose deficiency limits the growth and development of plants. In this study, we investigated the possible role of GmWRKY46 in the phosphate (Pi) starvation stress tolerance of soybean. GmWRKY46 belonged to the group III subfamily of the WRKY tra...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Cheng, Li, Kangning, Liu, Xinyi, Ruan, Hui, Zheng, Mingming, Yu, Zhijie, Gai, Junyi, Yang, Shouping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477037/
https://www.ncbi.nlm.nih.gov/pubmed/34594347
http://dx.doi.org/10.3389/fpls.2021.700651
_version_ 1784575756707299328
author Li, Cheng
Li, Kangning
Liu, Xinyi
Ruan, Hui
Zheng, Mingming
Yu, Zhijie
Gai, Junyi
Yang, Shouping
author_facet Li, Cheng
Li, Kangning
Liu, Xinyi
Ruan, Hui
Zheng, Mingming
Yu, Zhijie
Gai, Junyi
Yang, Shouping
author_sort Li, Cheng
collection PubMed
description Phosphorus (P) is one of the essential macronutrients, whose deficiency limits the growth and development of plants. In this study, we investigated the possible role of GmWRKY46 in the phosphate (Pi) starvation stress tolerance of soybean. GmWRKY46 belonged to the group III subfamily of the WRKY transcription factor family, which was localized in the nucleus and had transcriptional activator activity. GmWRKY46 could be strongly induced by Pi starvation, especially in soybean roots. Overexpression of GmWRKY46 significantly enhanced tolerance to Pi starvation and lateral root development in transgenic Arabidopsis. RNA-seq analysis showed that overexpression of GmWRKY46 led to change in many genes related to energy metabolisms, stress responses, and plant hormone signal transduction in transgenic Arabidopsis. Among these differential expression genes, we found that overexpression of AtAED1 alone could enhance the tolerance of transgenic Arabidopsis to Pi starvation. Y1H and ChIP-qPCR analyses showed that GmWRKY46 could directly bind to the W-box motif of the AtAED1 promoter in vitro and in vivo. Furthermore, results from intact soybean composite plants with GmWRKY46 overexpression showed that GmWRKY46 was involved in hairy roots development and subsequently affected plant growth and Pi uptake. These results provide a basis for the molecular genetic breeding of soybean tolerant to Pi starvation.
format Online
Article
Text
id pubmed-8477037
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84770372021-09-29 Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants Li, Cheng Li, Kangning Liu, Xinyi Ruan, Hui Zheng, Mingming Yu, Zhijie Gai, Junyi Yang, Shouping Front Plant Sci Plant Science Phosphorus (P) is one of the essential macronutrients, whose deficiency limits the growth and development of plants. In this study, we investigated the possible role of GmWRKY46 in the phosphate (Pi) starvation stress tolerance of soybean. GmWRKY46 belonged to the group III subfamily of the WRKY transcription factor family, which was localized in the nucleus and had transcriptional activator activity. GmWRKY46 could be strongly induced by Pi starvation, especially in soybean roots. Overexpression of GmWRKY46 significantly enhanced tolerance to Pi starvation and lateral root development in transgenic Arabidopsis. RNA-seq analysis showed that overexpression of GmWRKY46 led to change in many genes related to energy metabolisms, stress responses, and plant hormone signal transduction in transgenic Arabidopsis. Among these differential expression genes, we found that overexpression of AtAED1 alone could enhance the tolerance of transgenic Arabidopsis to Pi starvation. Y1H and ChIP-qPCR analyses showed that GmWRKY46 could directly bind to the W-box motif of the AtAED1 promoter in vitro and in vivo. Furthermore, results from intact soybean composite plants with GmWRKY46 overexpression showed that GmWRKY46 was involved in hairy roots development and subsequently affected plant growth and Pi uptake. These results provide a basis for the molecular genetic breeding of soybean tolerant to Pi starvation. Frontiers Media S.A. 2021-09-14 /pmc/articles/PMC8477037/ /pubmed/34594347 http://dx.doi.org/10.3389/fpls.2021.700651 Text en Copyright © 2021 Li, Li, Liu, Ruan, Zheng, Yu, Gai and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Cheng
Li, Kangning
Liu, Xinyi
Ruan, Hui
Zheng, Mingming
Yu, Zhijie
Gai, Junyi
Yang, Shouping
Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
title Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
title_full Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
title_fullStr Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
title_full_unstemmed Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
title_short Transcription Factor GmWRKY46 Enhanced Phosphate Starvation Tolerance and Root Development in Transgenic Plants
title_sort transcription factor gmwrky46 enhanced phosphate starvation tolerance and root development in transgenic plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477037/
https://www.ncbi.nlm.nih.gov/pubmed/34594347
http://dx.doi.org/10.3389/fpls.2021.700651
work_keys_str_mv AT licheng transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT likangning transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT liuxinyi transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT ruanhui transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT zhengmingming transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT yuzhijie transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT gaijunyi transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants
AT yangshouping transcriptionfactorgmwrky46enhancedphosphatestarvationtoleranceandrootdevelopmentintransgenicplants