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Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad

Travel to tropical regions is associated with high risk of acquiring extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) that are typically cleared in less than 3 months following return. The conditions leading to persistent carriage that exceeds 3 months in some travellers require...

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Autores principales: Armand-Lefèvre, Laurence, Rondinaud, Emilie, Desvillechabrol, Dimitri, Mullaert, Jimmy, Clermont, Olivier, Petitjean, Marie, Ruppe, Etienne, Cokelaer, Thomas, Bouchier, Christiane, Tenaillon, Olivier, Ma, Laurence, Nooroya, Yasmine, Matheron, Sophie, Andremont, Antoine, Denamur, Erick, Kennedy, Sean P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477403/
https://www.ncbi.nlm.nih.gov/pubmed/34279212
http://dx.doi.org/10.1099/mgen.0.000576
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author Armand-Lefèvre, Laurence
Rondinaud, Emilie
Desvillechabrol, Dimitri
Mullaert, Jimmy
Clermont, Olivier
Petitjean, Marie
Ruppe, Etienne
Cokelaer, Thomas
Bouchier, Christiane
Tenaillon, Olivier
Ma, Laurence
Nooroya, Yasmine
Matheron, Sophie
Andremont, Antoine
Denamur, Erick
Kennedy, Sean P.
author_facet Armand-Lefèvre, Laurence
Rondinaud, Emilie
Desvillechabrol, Dimitri
Mullaert, Jimmy
Clermont, Olivier
Petitjean, Marie
Ruppe, Etienne
Cokelaer, Thomas
Bouchier, Christiane
Tenaillon, Olivier
Ma, Laurence
Nooroya, Yasmine
Matheron, Sophie
Andremont, Antoine
Denamur, Erick
Kennedy, Sean P.
author_sort Armand-Lefèvre, Laurence
collection PubMed
description Travel to tropical regions is associated with high risk of acquiring extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) that are typically cleared in less than 3 months following return. The conditions leading to persistent carriage that exceeds 3 months in some travellers require investigation. Whole-genome sequencing (Illumina MiSeq) was performed on the 82 ESBL-E isolates detected upon return and 1, 2, 3, 6 and 12 months later from the stools of 11 long-term (>3 months) ESBL-E carriers following travel abroad. One to five different ESBL Escherichia coli strains were detected per traveller upon return, and this diminished to one after 3 months. Long-term carriage was due to the presence of the same ESBL E. coli strain, for more than 3 months, in 9 out of 11 travellers, belonging to epidemic sequence type complexes (STc 10, 14, 38, 69, 131 and 648). The mean carriage duration of strains belonging to phylogroups B2/D/F, associated with extra-intestinal virulence, was higher than that for commensal-associated A/B1/E phylogroups (3.5 vs 0.5 months, P=0.021). Genes encoding iron capture systems (fyuA, irp), toxins (senB, sat), adhesins (flu, daaF, afa/nfaE, pap, ecpA) and colicin (cjrA) were more often present in persistent strains than in transient ones. Single-nucleotide polymorphism (SNP) analysis in persistent strains showed a maximum divergence of eight SNPs over 12 months without signs of adaptation. Genomic plasticity was observed during the follow-up with the loss or gain of mobile genetic elements such as plasmids, integrons and/or transposons that may contain resistance genes at different points in the follow-up. Long-term colonization of ESBL-E following travel is primarily due to the acquisition of E. coli strains belonging to epidemic clones and harbouring ‘virulence genes’, allowing good adaptation to the intestinal microbiota.
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spelling pubmed-84774032021-09-28 Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad Armand-Lefèvre, Laurence Rondinaud, Emilie Desvillechabrol, Dimitri Mullaert, Jimmy Clermont, Olivier Petitjean, Marie Ruppe, Etienne Cokelaer, Thomas Bouchier, Christiane Tenaillon, Olivier Ma, Laurence Nooroya, Yasmine Matheron, Sophie Andremont, Antoine Denamur, Erick Kennedy, Sean P. Microb Genom Research Articles Travel to tropical regions is associated with high risk of acquiring extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) that are typically cleared in less than 3 months following return. The conditions leading to persistent carriage that exceeds 3 months in some travellers require investigation. Whole-genome sequencing (Illumina MiSeq) was performed on the 82 ESBL-E isolates detected upon return and 1, 2, 3, 6 and 12 months later from the stools of 11 long-term (>3 months) ESBL-E carriers following travel abroad. One to five different ESBL Escherichia coli strains were detected per traveller upon return, and this diminished to one after 3 months. Long-term carriage was due to the presence of the same ESBL E. coli strain, for more than 3 months, in 9 out of 11 travellers, belonging to epidemic sequence type complexes (STc 10, 14, 38, 69, 131 and 648). The mean carriage duration of strains belonging to phylogroups B2/D/F, associated with extra-intestinal virulence, was higher than that for commensal-associated A/B1/E phylogroups (3.5 vs 0.5 months, P=0.021). Genes encoding iron capture systems (fyuA, irp), toxins (senB, sat), adhesins (flu, daaF, afa/nfaE, pap, ecpA) and colicin (cjrA) were more often present in persistent strains than in transient ones. Single-nucleotide polymorphism (SNP) analysis in persistent strains showed a maximum divergence of eight SNPs over 12 months without signs of adaptation. Genomic plasticity was observed during the follow-up with the loss or gain of mobile genetic elements such as plasmids, integrons and/or transposons that may contain resistance genes at different points in the follow-up. Long-term colonization of ESBL-E following travel is primarily due to the acquisition of E. coli strains belonging to epidemic clones and harbouring ‘virulence genes’, allowing good adaptation to the intestinal microbiota. Microbiology Society 2021-07-19 /pmc/articles/PMC8477403/ /pubmed/34279212 http://dx.doi.org/10.1099/mgen.0.000576 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Articles
Armand-Lefèvre, Laurence
Rondinaud, Emilie
Desvillechabrol, Dimitri
Mullaert, Jimmy
Clermont, Olivier
Petitjean, Marie
Ruppe, Etienne
Cokelaer, Thomas
Bouchier, Christiane
Tenaillon, Olivier
Ma, Laurence
Nooroya, Yasmine
Matheron, Sophie
Andremont, Antoine
Denamur, Erick
Kennedy, Sean P.
Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad
title Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad
title_full Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad
title_fullStr Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad
title_full_unstemmed Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad
title_short Dynamics of extended-spectrum beta-lactamase-producing Enterobacterales colonization in long-term carriers following travel abroad
title_sort dynamics of extended-spectrum beta-lactamase-producing enterobacterales colonization in long-term carriers following travel abroad
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477403/
https://www.ncbi.nlm.nih.gov/pubmed/34279212
http://dx.doi.org/10.1099/mgen.0.000576
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