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Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey

Pseudomonas is a highly diverse genus that includes species that cause disease in both plants and animals. Recently, pathogenic pseudomonads from the Pseudomonas syringae and Pseudomonas fluorescens species complexes have caused significant outbreaks in several agronomically important crops in Turke...

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Autores principales: Dillon, Marcus M., Ruiz-Bedoya, Tatiana, Bundalovic-Torma, Cedoljub, Guttman, Kevin M., Kwak, Haejin, Middleton, Maggie A., Wang, Pauline W., Horuz, Sumer, Aysan, Yesim, Guttman, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477409/
https://www.ncbi.nlm.nih.gov/pubmed/34227931
http://dx.doi.org/10.1099/mgen.0.000585
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author Dillon, Marcus M.
Ruiz-Bedoya, Tatiana
Bundalovic-Torma, Cedoljub
Guttman, Kevin M.
Kwak, Haejin
Middleton, Maggie A.
Wang, Pauline W.
Horuz, Sumer
Aysan, Yesim
Guttman, David S.
author_facet Dillon, Marcus M.
Ruiz-Bedoya, Tatiana
Bundalovic-Torma, Cedoljub
Guttman, Kevin M.
Kwak, Haejin
Middleton, Maggie A.
Wang, Pauline W.
Horuz, Sumer
Aysan, Yesim
Guttman, David S.
author_sort Dillon, Marcus M.
collection PubMed
description Pseudomonas is a highly diverse genus that includes species that cause disease in both plants and animals. Recently, pathogenic pseudomonads from the Pseudomonas syringae and Pseudomonas fluorescens species complexes have caused significant outbreaks in several agronomically important crops in Turkey, including tomato, citrus, artichoke and melon. We characterized 169 pathogenic Pseudomonas strains associated with recent outbreaks in Turkey via multilocus sequence analysis and whole-genome sequencing, then used comparative and evolutionary genomics to characterize putative virulence mechanisms. Most of the isolates are closely related to other plant pathogens distributed among the primary phylogroups of P. syringae, although there are significant numbers of P. fluorescens isolates, which is a species better known as a rhizosphere-inhabiting plant-growth promoter. We found that all 39 citrus blast pathogens cluster in P. syringae phylogroup 2, although strains isolated from the same host do not cluster monophyletically, with lemon, mandarin orange and sweet orange isolates all being intermixed throughout the phylogroup. In contrast, 20 tomato pith pathogens are found in two independent lineages: one in the P. syringae secondary phylogroups, and the other from the P. fluorescens species complex. These divergent pith necrosis strains lack characteristic virulence factors like the canonical tripartite type III secretion system, large effector repertoires and the ability to synthesize multiple bacterial phytotoxins, suggesting they have alternative molecular mechanisms to cause disease. These findings highlight the complex nature of host specificity among plant pathogenic pseudomonads.
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spelling pubmed-84774092021-09-28 Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey Dillon, Marcus M. Ruiz-Bedoya, Tatiana Bundalovic-Torma, Cedoljub Guttman, Kevin M. Kwak, Haejin Middleton, Maggie A. Wang, Pauline W. Horuz, Sumer Aysan, Yesim Guttman, David S. Microb Genom Research Articles Pseudomonas is a highly diverse genus that includes species that cause disease in both plants and animals. Recently, pathogenic pseudomonads from the Pseudomonas syringae and Pseudomonas fluorescens species complexes have caused significant outbreaks in several agronomically important crops in Turkey, including tomato, citrus, artichoke and melon. We characterized 169 pathogenic Pseudomonas strains associated with recent outbreaks in Turkey via multilocus sequence analysis and whole-genome sequencing, then used comparative and evolutionary genomics to characterize putative virulence mechanisms. Most of the isolates are closely related to other plant pathogens distributed among the primary phylogroups of P. syringae, although there are significant numbers of P. fluorescens isolates, which is a species better known as a rhizosphere-inhabiting plant-growth promoter. We found that all 39 citrus blast pathogens cluster in P. syringae phylogroup 2, although strains isolated from the same host do not cluster monophyletically, with lemon, mandarin orange and sweet orange isolates all being intermixed throughout the phylogroup. In contrast, 20 tomato pith pathogens are found in two independent lineages: one in the P. syringae secondary phylogroups, and the other from the P. fluorescens species complex. These divergent pith necrosis strains lack characteristic virulence factors like the canonical tripartite type III secretion system, large effector repertoires and the ability to synthesize multiple bacterial phytotoxins, suggesting they have alternative molecular mechanisms to cause disease. These findings highlight the complex nature of host specificity among plant pathogenic pseudomonads. Microbiology Society 2021-07-06 /pmc/articles/PMC8477409/ /pubmed/34227931 http://dx.doi.org/10.1099/mgen.0.000585 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Articles
Dillon, Marcus M.
Ruiz-Bedoya, Tatiana
Bundalovic-Torma, Cedoljub
Guttman, Kevin M.
Kwak, Haejin
Middleton, Maggie A.
Wang, Pauline W.
Horuz, Sumer
Aysan, Yesim
Guttman, David S.
Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey
title Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey
title_full Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey
title_fullStr Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey
title_full_unstemmed Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey
title_short Comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from Turkey
title_sort comparative genomic insights into the epidemiology and virulence of plant pathogenic pseudomonads from turkey
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477409/
https://www.ncbi.nlm.nih.gov/pubmed/34227931
http://dx.doi.org/10.1099/mgen.0.000585
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