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MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression

Gastric cancer (GC) accounts for a main cause of cancer-related deaths. This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC. Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA databa...

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Autores principales: Li, Jianjie, Xu, Xiaoyue, Liu, Chunhui, Xi, Xiaoxue, Wang, Yang, Wu, Xiaotang, Li, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478551/
https://www.ncbi.nlm.nih.gov/pubmed/34594374
http://dx.doi.org/10.1155/2021/2894117
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author Li, Jianjie
Xu, Xiaoyue
Liu, Chunhui
Xi, Xiaoxue
Wang, Yang
Wu, Xiaotang
Li, Hua
author_facet Li, Jianjie
Xu, Xiaoyue
Liu, Chunhui
Xi, Xiaoxue
Wang, Yang
Wu, Xiaotang
Li, Hua
author_sort Li, Jianjie
collection PubMed
description Gastric cancer (GC) accounts for a main cause of cancer-related deaths. This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC. Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA database were analyzed. MiR-490-5p and DTL mRNA expression levels in GC were evaluated with qRT-PCR. Cell viability was confirmed with CCK-8 method. Cell cycle distribution and apoptosis were analyzed with flow cytometry. Cell migratory and invasive potential was proved with Transwell assay. The targeted relationship between DTL and miR-490-5p was analyzed with dual-luciferase assay. The results indicated a decreased miR-490-5p level in GC cells. MiR-490-5p upregulation hampered proliferation, migration, invasion and promote cell apoptosis. DTL was the target of and inversely associated with miR-490-5p, and it could remarkably induce the carcinogenesis of GC. MiR-490-5p mediated GC cell progression by DTL repression. In conclusion, miR-490-5p and DTL may be valuable in diagnosis and treatment for GC.
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spelling pubmed-84785512021-09-29 MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression Li, Jianjie Xu, Xiaoyue Liu, Chunhui Xi, Xiaoxue Wang, Yang Wu, Xiaotang Li, Hua Gastroenterol Res Pract Research Article Gastric cancer (GC) accounts for a main cause of cancer-related deaths. This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC. Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA database were analyzed. MiR-490-5p and DTL mRNA expression levels in GC were evaluated with qRT-PCR. Cell viability was confirmed with CCK-8 method. Cell cycle distribution and apoptosis were analyzed with flow cytometry. Cell migratory and invasive potential was proved with Transwell assay. The targeted relationship between DTL and miR-490-5p was analyzed with dual-luciferase assay. The results indicated a decreased miR-490-5p level in GC cells. MiR-490-5p upregulation hampered proliferation, migration, invasion and promote cell apoptosis. DTL was the target of and inversely associated with miR-490-5p, and it could remarkably induce the carcinogenesis of GC. MiR-490-5p mediated GC cell progression by DTL repression. In conclusion, miR-490-5p and DTL may be valuable in diagnosis and treatment for GC. Hindawi 2021-09-20 /pmc/articles/PMC8478551/ /pubmed/34594374 http://dx.doi.org/10.1155/2021/2894117 Text en Copyright © 2021 Jianjie Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Jianjie
Xu, Xiaoyue
Liu, Chunhui
Xi, Xiaoxue
Wang, Yang
Wu, Xiaotang
Li, Hua
MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
title MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
title_full MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
title_fullStr MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
title_full_unstemmed MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
title_short MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression
title_sort mir-490-5p restrains progression of gastric cancer through dtl repression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478551/
https://www.ncbi.nlm.nih.gov/pubmed/34594374
http://dx.doi.org/10.1155/2021/2894117
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