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Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia

Inflammation is the pathophysiological basis of hyperlipidemia-related disease (HRD). Reducing the south and reinforcing the north method (RSRN) has a positive effect on HRD. However, the pharmacological mechanisms of RSRN are still unclear in the treatment of HRD. We obtained RSRN compounds from th...

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Autores principales: Wu, Hongjin, Dai, Weiwei, Wang, Libo, Zhang, Jie, Wang, Chenglong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478564/
https://www.ncbi.nlm.nih.gov/pubmed/34594388
http://dx.doi.org/10.1155/2021/1860508
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author Wu, Hongjin
Dai, Weiwei
Wang, Libo
Zhang, Jie
Wang, Chenglong
author_facet Wu, Hongjin
Dai, Weiwei
Wang, Libo
Zhang, Jie
Wang, Chenglong
author_sort Wu, Hongjin
collection PubMed
description Inflammation is the pathophysiological basis of hyperlipidemia-related disease (HRD). Reducing the south and reinforcing the north method (RSRN) has a positive effect on HRD. However, the pharmacological mechanisms of RSRN are still unclear in the treatment of HRD. We obtained RSRN compounds from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) and identified potential targets of these compounds through target fishing based on the TCMSP databases. Next, we identified the HRD targets by using multiple databases. Then, the overlapping genes between the RSRN potential targets and the HRD targets were used to establish a protein-protein interaction (PPI) network, and we further analyzed their interactions and identified the major hub genes in this network. Subsequently, the Metascape database was utilized to conduct the enrichment of Gene Ontology biological processes (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. A total of 187 potential active components and 106 related core targets were obtained and identified overall. Then after the Metascape enrichment analysis, a total of 148 KEGG pathways were screened, which were mainly associated with AGE-RAGE signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway, and NF-kappa B signaling pathway. Furthermore, 34 hub genes, such as AKT1, NF-κBp65(RELA), IκBα(CHUK), MAPK8, and MAPK14, CCND1, were considered potential therapeutic targets. Furthermore, evaluations of protein levels of NF-κBp65, IκBα, TNF-α, IL-1 ß, and IL-6 were performed for experimental validation. RSRN can reduce the expression of NF-κBp65 protein, increase the level of IκBα protein, and reduce the protein levels of TNF-α, IL-1β, and IL-6 in ovariectomized rats. The results indicate that the mechanism of RSRN against inflammation may be related to AKT1, NF-κBp65, IκBα, MAPK8, and MAPK14, as well as TNF, NF-kappa B, PI3K-Akt signaling pathways.
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spelling pubmed-84785642021-09-29 Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia Wu, Hongjin Dai, Weiwei Wang, Libo Zhang, Jie Wang, Chenglong Evid Based Complement Alternat Med Research Article Inflammation is the pathophysiological basis of hyperlipidemia-related disease (HRD). Reducing the south and reinforcing the north method (RSRN) has a positive effect on HRD. However, the pharmacological mechanisms of RSRN are still unclear in the treatment of HRD. We obtained RSRN compounds from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) and identified potential targets of these compounds through target fishing based on the TCMSP databases. Next, we identified the HRD targets by using multiple databases. Then, the overlapping genes between the RSRN potential targets and the HRD targets were used to establish a protein-protein interaction (PPI) network, and we further analyzed their interactions and identified the major hub genes in this network. Subsequently, the Metascape database was utilized to conduct the enrichment of Gene Ontology biological processes (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. A total of 187 potential active components and 106 related core targets were obtained and identified overall. Then after the Metascape enrichment analysis, a total of 148 KEGG pathways were screened, which were mainly associated with AGE-RAGE signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway, and NF-kappa B signaling pathway. Furthermore, 34 hub genes, such as AKT1, NF-κBp65(RELA), IκBα(CHUK), MAPK8, and MAPK14, CCND1, were considered potential therapeutic targets. Furthermore, evaluations of protein levels of NF-κBp65, IκBα, TNF-α, IL-1 ß, and IL-6 were performed for experimental validation. RSRN can reduce the expression of NF-κBp65 protein, increase the level of IκBα protein, and reduce the protein levels of TNF-α, IL-1β, and IL-6 in ovariectomized rats. The results indicate that the mechanism of RSRN against inflammation may be related to AKT1, NF-κBp65, IκBα, MAPK8, and MAPK14, as well as TNF, NF-kappa B, PI3K-Akt signaling pathways. Hindawi 2021-09-20 /pmc/articles/PMC8478564/ /pubmed/34594388 http://dx.doi.org/10.1155/2021/1860508 Text en Copyright © 2021 Hongjin Wu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Hongjin
Dai, Weiwei
Wang, Libo
Zhang, Jie
Wang, Chenglong
Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia
title Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia
title_full Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia
title_fullStr Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia
title_full_unstemmed Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia
title_short Effects of Reducing the South and Reinforcing the North Method on Inflammatory Injury Induced by Hyperlipidemia
title_sort effects of reducing the south and reinforcing the north method on inflammatory injury induced by hyperlipidemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478564/
https://www.ncbi.nlm.nih.gov/pubmed/34594388
http://dx.doi.org/10.1155/2021/1860508
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