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Enhancing esophageal repair with bioactive bilayer mesh containing FGF

We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly ε-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in vivo model w...

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Autores principales: Cesur, Ozkan, Tanir, Tugba Endogan, Celepli, Pinar, Ozarslan, Fatma, Hucumenoglu, Sema, Karaibrahimoglu, Adnan, Hasirci, Nesrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478899/
https://www.ncbi.nlm.nih.gov/pubmed/34584186
http://dx.doi.org/10.1038/s41598-021-98840-w
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author Cesur, Ozkan
Tanir, Tugba Endogan
Celepli, Pinar
Ozarslan, Fatma
Hucumenoglu, Sema
Karaibrahimoglu, Adnan
Hasirci, Nesrin
author_facet Cesur, Ozkan
Tanir, Tugba Endogan
Celepli, Pinar
Ozarslan, Fatma
Hucumenoglu, Sema
Karaibrahimoglu, Adnan
Hasirci, Nesrin
author_sort Cesur, Ozkan
collection PubMed
description We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly ε-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in vivo model would improve tissue healing in rats. The full thickness semicircular defects of 0.5 × 0.5 cm(2) were created in anterior walls of abdominal esophagus. The control group had abdominal esophagus isolated with distal esophageal blunt dissection, and sham group had primary anastomosis. In the test groups, the defects were covered with bilayer polymeric meshes containing FGF (5 μg/2 cm(2)), or not. All rats were sacrificed for histopathology investigation after 7 or 28 days of operation. The groups are coded as FGF(−)-7th day, FGF(+)-7th day, and FGF(+)-28th day, based on their content and operation day. Highest burst pressures were obtained for FGF(+)-7th day, and FGF(+)-28th day groups (p < 0.005) and decreased inflammation grades were observed. Submucosal and muscular collagen deposition scores were markedly increased in these groups compared to sham and FGF(−)-7th day groups having no FGF (p = 0.002, p = 0.001, respectively). It was proved that FGF loaded bioactive bilayer mesh provided effective repair, reinforcement and tissue healing of esophageal defects.
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spelling pubmed-84788992021-09-29 Enhancing esophageal repair with bioactive bilayer mesh containing FGF Cesur, Ozkan Tanir, Tugba Endogan Celepli, Pinar Ozarslan, Fatma Hucumenoglu, Sema Karaibrahimoglu, Adnan Hasirci, Nesrin Sci Rep Article We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly ε-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in vivo model would improve tissue healing in rats. The full thickness semicircular defects of 0.5 × 0.5 cm(2) were created in anterior walls of abdominal esophagus. The control group had abdominal esophagus isolated with distal esophageal blunt dissection, and sham group had primary anastomosis. In the test groups, the defects were covered with bilayer polymeric meshes containing FGF (5 μg/2 cm(2)), or not. All rats were sacrificed for histopathology investigation after 7 or 28 days of operation. The groups are coded as FGF(−)-7th day, FGF(+)-7th day, and FGF(+)-28th day, based on their content and operation day. Highest burst pressures were obtained for FGF(+)-7th day, and FGF(+)-28th day groups (p < 0.005) and decreased inflammation grades were observed. Submucosal and muscular collagen deposition scores were markedly increased in these groups compared to sham and FGF(−)-7th day groups having no FGF (p = 0.002, p = 0.001, respectively). It was proved that FGF loaded bioactive bilayer mesh provided effective repair, reinforcement and tissue healing of esophageal defects. Nature Publishing Group UK 2021-09-28 /pmc/articles/PMC8478899/ /pubmed/34584186 http://dx.doi.org/10.1038/s41598-021-98840-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cesur, Ozkan
Tanir, Tugba Endogan
Celepli, Pinar
Ozarslan, Fatma
Hucumenoglu, Sema
Karaibrahimoglu, Adnan
Hasirci, Nesrin
Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_full Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_fullStr Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_full_unstemmed Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_short Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_sort enhancing esophageal repair with bioactive bilayer mesh containing fgf
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478899/
https://www.ncbi.nlm.nih.gov/pubmed/34584186
http://dx.doi.org/10.1038/s41598-021-98840-w
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