Cargando…

Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma

Important evidence indicates that the microbiota plays a key role in esophageal squamous cell carcinoma (ESCC). Here, paired saliva and brush specimens were obtained from 276 participants undergoing upper gastrointestinal endoscopic examination before or during screening for upper gastrointestinal (...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Zhengqi, Dou, Lizhou, Zhang, Yueming, He, Shun, Zhao, Deli, Hao, Changqing, Song, Guohui, Zhang, Wei, Liu, Yong, Wang, Guiqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479167/
https://www.ncbi.nlm.nih.gov/pubmed/34604107
http://dx.doi.org/10.3389/fcimb.2021.714162
_version_ 1784576192085491712
author Li, Zhengqi
Dou, Lizhou
Zhang, Yueming
He, Shun
Zhao, Deli
Hao, Changqing
Song, Guohui
Zhang, Wei
Liu, Yong
Wang, Guiqi
author_facet Li, Zhengqi
Dou, Lizhou
Zhang, Yueming
He, Shun
Zhao, Deli
Hao, Changqing
Song, Guohui
Zhang, Wei
Liu, Yong
Wang, Guiqi
author_sort Li, Zhengqi
collection PubMed
description Important evidence indicates that the microbiota plays a key role in esophageal squamous cell carcinoma (ESCC). Here, paired saliva and brush specimens were obtained from 276 participants undergoing upper gastrointestinal endoscopic examination before or during screening for upper gastrointestinal (UGI) cancer. The esophageal microbiota was investigated by 16S rRNA gene profiling and next-generation sequencing. We observed that as the disease progressed, the α diversity in the saliva and cell brush samples decreased. Linear discriminant analysis effect size (LEfSe) results showed that in both the saliva and cell brush specimens, Granulicatella, Rothia, Streptococcus, Gemella, Leptotrichia and Schaalia were common biomarkers in patients with low-grade dysplasia, Lactobacillus was a common biomarker in patients with high-grade dysplasia, and Bosea, Solobacterium, Gemella, and Peptostreptococcus were common biomarkers in patients with esophageal cancer. The top 3 genera in the saliva and cell brush specimens had areas under the curve (AUCs) of 87.16 and 89.13%, respectively, to distinguish ESCC patients from normal people. The PICRUSt2 results identified in brush samples that patients with ESCC had decreased nitrate reductase functions. Our results suggest that future studies can focus on the function of the characteristic bacteria in ESCC.
format Online
Article
Text
id pubmed-8479167
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84791672021-09-30 Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma Li, Zhengqi Dou, Lizhou Zhang, Yueming He, Shun Zhao, Deli Hao, Changqing Song, Guohui Zhang, Wei Liu, Yong Wang, Guiqi Front Cell Infect Microbiol Cellular and Infection Microbiology Important evidence indicates that the microbiota plays a key role in esophageal squamous cell carcinoma (ESCC). Here, paired saliva and brush specimens were obtained from 276 participants undergoing upper gastrointestinal endoscopic examination before or during screening for upper gastrointestinal (UGI) cancer. The esophageal microbiota was investigated by 16S rRNA gene profiling and next-generation sequencing. We observed that as the disease progressed, the α diversity in the saliva and cell brush samples decreased. Linear discriminant analysis effect size (LEfSe) results showed that in both the saliva and cell brush specimens, Granulicatella, Rothia, Streptococcus, Gemella, Leptotrichia and Schaalia were common biomarkers in patients with low-grade dysplasia, Lactobacillus was a common biomarker in patients with high-grade dysplasia, and Bosea, Solobacterium, Gemella, and Peptostreptococcus were common biomarkers in patients with esophageal cancer. The top 3 genera in the saliva and cell brush specimens had areas under the curve (AUCs) of 87.16 and 89.13%, respectively, to distinguish ESCC patients from normal people. The PICRUSt2 results identified in brush samples that patients with ESCC had decreased nitrate reductase functions. Our results suggest that future studies can focus on the function of the characteristic bacteria in ESCC. Frontiers Media S.A. 2021-09-15 /pmc/articles/PMC8479167/ /pubmed/34604107 http://dx.doi.org/10.3389/fcimb.2021.714162 Text en Copyright © 2021 Li, Dou, Zhang, He, Zhao, Hao, Song, Zhang, Liu and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Zhengqi
Dou, Lizhou
Zhang, Yueming
He, Shun
Zhao, Deli
Hao, Changqing
Song, Guohui
Zhang, Wei
Liu, Yong
Wang, Guiqi
Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma
title Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma
title_full Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma
title_fullStr Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma
title_full_unstemmed Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma
title_short Characterization of the Oral and Esophageal Microbiota in Esophageal Precancerous Lesions and Squamous Cell Carcinoma
title_sort characterization of the oral and esophageal microbiota in esophageal precancerous lesions and squamous cell carcinoma
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479167/
https://www.ncbi.nlm.nih.gov/pubmed/34604107
http://dx.doi.org/10.3389/fcimb.2021.714162
work_keys_str_mv AT lizhengqi characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT doulizhou characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT zhangyueming characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT heshun characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT zhaodeli characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT haochangqing characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT songguohui characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT zhangwei characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT liuyong characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma
AT wangguiqi characterizationoftheoralandesophagealmicrobiotainesophagealprecancerouslesionsandsquamouscellcarcinoma