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An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies

Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics. Most T cell cloning methods involve a stringent singularization process, which necessitates either tedious han...

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Autores principales: Chen, Lei, Dong, Lianhua, Ma, Yipeng, Wang, Juntao, Qiao, Dongjuan, Tian, Geng, Wang, Mingjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480097/
https://www.ncbi.nlm.nih.gov/pubmed/34583647
http://dx.doi.org/10.1186/s12865-021-00455-3
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author Chen, Lei
Dong, Lianhua
Ma, Yipeng
Wang, Juntao
Qiao, Dongjuan
Tian, Geng
Wang, Mingjun
author_facet Chen, Lei
Dong, Lianhua
Ma, Yipeng
Wang, Juntao
Qiao, Dongjuan
Tian, Geng
Wang, Mingjun
author_sort Chen, Lei
collection PubMed
description Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics. Most T cell cloning methods involve a stringent singularization process, which necessitates either tedious hands-on operations or high cost. We present an efficient and nonstringent cloning approach based on existing techniques. We hypothesize that after elimination of most nonspecific T cells, a clonotype with high quality could outcompete other clonotypes and finally form a predominant population. This TCR identification method can be used to clone virus-specific TCRs efficiently from cancer patients and is easily adoptable by any laboratory. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00455-3.
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spelling pubmed-84800972021-09-30 An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies Chen, Lei Dong, Lianhua Ma, Yipeng Wang, Juntao Qiao, Dongjuan Tian, Geng Wang, Mingjun BMC Immunol Research Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics. Most T cell cloning methods involve a stringent singularization process, which necessitates either tedious hands-on operations or high cost. We present an efficient and nonstringent cloning approach based on existing techniques. We hypothesize that after elimination of most nonspecific T cells, a clonotype with high quality could outcompete other clonotypes and finally form a predominant population. This TCR identification method can be used to clone virus-specific TCRs efficiently from cancer patients and is easily adoptable by any laboratory. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00455-3. BioMed Central 2021-09-28 /pmc/articles/PMC8480097/ /pubmed/34583647 http://dx.doi.org/10.1186/s12865-021-00455-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Lei
Dong, Lianhua
Ma, Yipeng
Wang, Juntao
Qiao, Dongjuan
Tian, Geng
Wang, Mingjun
An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
title An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
title_full An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
title_fullStr An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
title_full_unstemmed An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
title_short An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
title_sort efficient method to identify virus-specific tcrs for tcr-t cell immunotherapy against virus-associated malignancies
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480097/
https://www.ncbi.nlm.nih.gov/pubmed/34583647
http://dx.doi.org/10.1186/s12865-021-00455-3
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