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An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies
Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics. Most T cell cloning methods involve a stringent singularization process, which necessitates either tedious han...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480097/ https://www.ncbi.nlm.nih.gov/pubmed/34583647 http://dx.doi.org/10.1186/s12865-021-00455-3 |
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author | Chen, Lei Dong, Lianhua Ma, Yipeng Wang, Juntao Qiao, Dongjuan Tian, Geng Wang, Mingjun |
author_facet | Chen, Lei Dong, Lianhua Ma, Yipeng Wang, Juntao Qiao, Dongjuan Tian, Geng Wang, Mingjun |
author_sort | Chen, Lei |
collection | PubMed |
description | Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics. Most T cell cloning methods involve a stringent singularization process, which necessitates either tedious hands-on operations or high cost. We present an efficient and nonstringent cloning approach based on existing techniques. We hypothesize that after elimination of most nonspecific T cells, a clonotype with high quality could outcompete other clonotypes and finally form a predominant population. This TCR identification method can be used to clone virus-specific TCRs efficiently from cancer patients and is easily adoptable by any laboratory. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00455-3. |
format | Online Article Text |
id | pubmed-8480097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-84800972021-09-30 An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies Chen, Lei Dong, Lianhua Ma, Yipeng Wang, Juntao Qiao, Dongjuan Tian, Geng Wang, Mingjun BMC Immunol Research Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics. Most T cell cloning methods involve a stringent singularization process, which necessitates either tedious hands-on operations or high cost. We present an efficient and nonstringent cloning approach based on existing techniques. We hypothesize that after elimination of most nonspecific T cells, a clonotype with high quality could outcompete other clonotypes and finally form a predominant population. This TCR identification method can be used to clone virus-specific TCRs efficiently from cancer patients and is easily adoptable by any laboratory. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00455-3. BioMed Central 2021-09-28 /pmc/articles/PMC8480097/ /pubmed/34583647 http://dx.doi.org/10.1186/s12865-021-00455-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Chen, Lei Dong, Lianhua Ma, Yipeng Wang, Juntao Qiao, Dongjuan Tian, Geng Wang, Mingjun An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies |
title | An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies |
title_full | An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies |
title_fullStr | An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies |
title_full_unstemmed | An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies |
title_short | An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies |
title_sort | efficient method to identify virus-specific tcrs for tcr-t cell immunotherapy against virus-associated malignancies |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480097/ https://www.ncbi.nlm.nih.gov/pubmed/34583647 http://dx.doi.org/10.1186/s12865-021-00455-3 |
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