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Imaging the oxygen wave with a single bioluminescent bacterium

We developed a capability of a monolayer of bioluminescent (BL) bacteria for spatiotemporally visualizing the heterogeneous distribution and dynamic evolution of interfacial oxygen concentration, resulting in the discovery of spontaneous and stochastic oxygen waves at the interface between the subst...

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Detalles Bibliográficos
Autores principales: Li, Yaohua, Wang, Sa, He, Xinyu, Li, Shijun, Zheng, Tianhua, Chen, You-Peng, Cui, Hua, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480313/
https://www.ncbi.nlm.nih.gov/pubmed/34603670
http://dx.doi.org/10.1039/d1sc03310g
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author Li, Yaohua
Wang, Sa
He, Xinyu
Li, Shijun
Zheng, Tianhua
Chen, You-Peng
Cui, Hua
Wang, Wei
author_facet Li, Yaohua
Wang, Sa
He, Xinyu
Li, Shijun
Zheng, Tianhua
Chen, You-Peng
Cui, Hua
Wang, Wei
author_sort Li, Yaohua
collection PubMed
description We developed a capability of a monolayer of bioluminescent (BL) bacteria for spatiotemporally visualizing the heterogeneous distribution and dynamic evolution of interfacial oxygen concentration, resulting in the discovery of spontaneous and stochastic oxygen waves at the interface between the substrate and an undisturbed, apparently still solution. Wild type bacteria, P. phosphoreum, spontaneously emit light during the native metabolism processes, i.e., bioluminescence. The emission intensity is sensitively regulated by oxygen concentration. By taking the electrolysis of water as a model, it was demonstrated that time-lapsed BL imaging of a bacterial monolayer allowed for visualizing the dynamic distribution of oxygen. The results were quantitatively understood with a physical model involving the diffusion equation and Michaelis–Menten equation. Unexpectedly, further study uncovered a spontaneous and stochastic oxygen wave in a standard well of a microtiter plate, which was subsequently attributed to the inevitable micro-convections induced by inhomogeneous evaporation and thermal fluctuation. Because of the wide application of microtiter plates, this study sheds new light to better understand the apparent heterogeneity in cell-culture and bio-assays.
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spelling pubmed-84803132021-10-01 Imaging the oxygen wave with a single bioluminescent bacterium Li, Yaohua Wang, Sa He, Xinyu Li, Shijun Zheng, Tianhua Chen, You-Peng Cui, Hua Wang, Wei Chem Sci Chemistry We developed a capability of a monolayer of bioluminescent (BL) bacteria for spatiotemporally visualizing the heterogeneous distribution and dynamic evolution of interfacial oxygen concentration, resulting in the discovery of spontaneous and stochastic oxygen waves at the interface between the substrate and an undisturbed, apparently still solution. Wild type bacteria, P. phosphoreum, spontaneously emit light during the native metabolism processes, i.e., bioluminescence. The emission intensity is sensitively regulated by oxygen concentration. By taking the electrolysis of water as a model, it was demonstrated that time-lapsed BL imaging of a bacterial monolayer allowed for visualizing the dynamic distribution of oxygen. The results were quantitatively understood with a physical model involving the diffusion equation and Michaelis–Menten equation. Unexpectedly, further study uncovered a spontaneous and stochastic oxygen wave in a standard well of a microtiter plate, which was subsequently attributed to the inevitable micro-convections induced by inhomogeneous evaporation and thermal fluctuation. Because of the wide application of microtiter plates, this study sheds new light to better understand the apparent heterogeneity in cell-culture and bio-assays. The Royal Society of Chemistry 2021-08-12 /pmc/articles/PMC8480313/ /pubmed/34603670 http://dx.doi.org/10.1039/d1sc03310g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Yaohua
Wang, Sa
He, Xinyu
Li, Shijun
Zheng, Tianhua
Chen, You-Peng
Cui, Hua
Wang, Wei
Imaging the oxygen wave with a single bioluminescent bacterium
title Imaging the oxygen wave with a single bioluminescent bacterium
title_full Imaging the oxygen wave with a single bioluminescent bacterium
title_fullStr Imaging the oxygen wave with a single bioluminescent bacterium
title_full_unstemmed Imaging the oxygen wave with a single bioluminescent bacterium
title_short Imaging the oxygen wave with a single bioluminescent bacterium
title_sort imaging the oxygen wave with a single bioluminescent bacterium
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480313/
https://www.ncbi.nlm.nih.gov/pubmed/34603670
http://dx.doi.org/10.1039/d1sc03310g
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