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Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples
Numerous studies have shown that droplet digital PCR (ddPCR) is a promising tool for the diagnosis of pathogens, especially in samples with low concentrations of pathogenic DNA. An early diagnosis of candidemia is critical for the effective treatment of patients. In this study, we evaluated the sens...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480469/ https://www.ncbi.nlm.nih.gov/pubmed/34603226 http://dx.doi.org/10.3389/fmicb.2021.700008 |
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author | Chen, Biao Xie, Yingguang Zhang, Ning Li, Wenqiang Liu, Chen Li, Dongmei Bian, Shaodong Jiang, Yufeng Yang, Zhiya Li, Renzhe Feng, Yahui Zhang, Xiaojie Shi, Dongmei |
author_facet | Chen, Biao Xie, Yingguang Zhang, Ning Li, Wenqiang Liu, Chen Li, Dongmei Bian, Shaodong Jiang, Yufeng Yang, Zhiya Li, Renzhe Feng, Yahui Zhang, Xiaojie Shi, Dongmei |
author_sort | Chen, Biao |
collection | PubMed |
description | Numerous studies have shown that droplet digital PCR (ddPCR) is a promising tool for the diagnosis of pathogens, especially in samples with low concentrations of pathogenic DNA. An early diagnosis of candidemia is critical for the effective treatment of patients. In this study, we evaluated the sensitivity and specificity of ddPCR assay for Candida DNA detection both in vitro by mixing fungal cells with human blood and in vivo by analyzing blood samples from infected mice and patients with suspected candidemia. The results showed that ddPCR assay could detect a minimum of 4.5 DNA copies per reaction in blood samples. ddPCR showed higher sensitivity and specificity for Candida DNA detection than traditional culture and quantitative PCR (qPCR) methods and also exhibited significantly better positive and negative predictive values than the culture and qPCR methods that were commonly used in clinical practice. Hence, our study demonstrates that ddPCR assay is a promising method for the timely diagnosis of candidemia and could be useful for monitoring the treatment of candidemia. |
format | Online Article Text |
id | pubmed-8480469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84804692021-09-30 Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples Chen, Biao Xie, Yingguang Zhang, Ning Li, Wenqiang Liu, Chen Li, Dongmei Bian, Shaodong Jiang, Yufeng Yang, Zhiya Li, Renzhe Feng, Yahui Zhang, Xiaojie Shi, Dongmei Front Microbiol Microbiology Numerous studies have shown that droplet digital PCR (ddPCR) is a promising tool for the diagnosis of pathogens, especially in samples with low concentrations of pathogenic DNA. An early diagnosis of candidemia is critical for the effective treatment of patients. In this study, we evaluated the sensitivity and specificity of ddPCR assay for Candida DNA detection both in vitro by mixing fungal cells with human blood and in vivo by analyzing blood samples from infected mice and patients with suspected candidemia. The results showed that ddPCR assay could detect a minimum of 4.5 DNA copies per reaction in blood samples. ddPCR showed higher sensitivity and specificity for Candida DNA detection than traditional culture and quantitative PCR (qPCR) methods and also exhibited significantly better positive and negative predictive values than the culture and qPCR methods that were commonly used in clinical practice. Hence, our study demonstrates that ddPCR assay is a promising method for the timely diagnosis of candidemia and could be useful for monitoring the treatment of candidemia. Frontiers Media S.A. 2021-09-03 /pmc/articles/PMC8480469/ /pubmed/34603226 http://dx.doi.org/10.3389/fmicb.2021.700008 Text en Copyright © 2021 Chen, Xie, Zhang, Li, Liu, Li, Bian, Jiang, Yang, Li, Feng, Zhang and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chen, Biao Xie, Yingguang Zhang, Ning Li, Wenqiang Liu, Chen Li, Dongmei Bian, Shaodong Jiang, Yufeng Yang, Zhiya Li, Renzhe Feng, Yahui Zhang, Xiaojie Shi, Dongmei Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples |
title | Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples |
title_full | Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples |
title_fullStr | Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples |
title_full_unstemmed | Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples |
title_short | Evaluation of Droplet Digital PCR Assay for the Diagnosis of Candidemia in Blood Samples |
title_sort | evaluation of droplet digital pcr assay for the diagnosis of candidemia in blood samples |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480469/ https://www.ncbi.nlm.nih.gov/pubmed/34603226 http://dx.doi.org/10.3389/fmicb.2021.700008 |
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