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Crowdsourced analysis of fungal growth and branching on microfluidic platforms
Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480888/ https://www.ncbi.nlm.nih.gov/pubmed/34587206 http://dx.doi.org/10.1371/journal.pone.0257823 |
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author | Hopke, Alex Mela, Alex Ellett, Felix Carter-House, Derreck Peña, Jesús F. Stajich, Jason E. Altamirano, Sophie Lovett, Brian Egan, Martin Kale, Shiv Kronholm, Ilkka Guerette, Paul Szewczyk, Edyta McCluskey, Kevin Breslauer, David Shah, Hiral Coad, Bryan R. Momany, Michelle Irimia, Daniel |
author_facet | Hopke, Alex Mela, Alex Ellett, Felix Carter-House, Derreck Peña, Jesús F. Stajich, Jason E. Altamirano, Sophie Lovett, Brian Egan, Martin Kale, Shiv Kronholm, Ilkka Guerette, Paul Szewczyk, Edyta McCluskey, Kevin Breslauer, David Shah, Hiral Coad, Bryan R. Momany, Michelle Irimia, Daniel |
author_sort | Hopke, Alex |
collection | PubMed |
description | Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of standardized metrics. Here, we used a microfluidic device featuring four different maze patterns to compare the growth velocity and branching frequency of fourteen filamentous fungi. These measurements result from the collective work of several labs in the form of a competition named the “Fungus Olympics.” The competing fungi included five ascomycete species (ten strains total), two basidiomycete species, and two zygomycete species. We found that growth velocity within a straight channel varied from 1 to 4 μm/min. We also found that the time to complete mazes when fungal hyphae branched or turned at various angles did not correlate with linear growth velocity. We discovered that fungi in our study used one of two distinct strategies to traverse mazes: high-frequency branching in which all possible paths were explored, and low-frequency branching in which only one or two paths were explored. While the high-frequency branching helped fungi escape mazes with sharp turns faster, the low-frequency turning had a significant advantage in mazes with shallower turns. Future work will more systematically examine these trends. |
format | Online Article Text |
id | pubmed-8480888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-84808882021-09-30 Crowdsourced analysis of fungal growth and branching on microfluidic platforms Hopke, Alex Mela, Alex Ellett, Felix Carter-House, Derreck Peña, Jesús F. Stajich, Jason E. Altamirano, Sophie Lovett, Brian Egan, Martin Kale, Shiv Kronholm, Ilkka Guerette, Paul Szewczyk, Edyta McCluskey, Kevin Breslauer, David Shah, Hiral Coad, Bryan R. Momany, Michelle Irimia, Daniel PLoS One Research Article Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of standardized metrics. Here, we used a microfluidic device featuring four different maze patterns to compare the growth velocity and branching frequency of fourteen filamentous fungi. These measurements result from the collective work of several labs in the form of a competition named the “Fungus Olympics.” The competing fungi included five ascomycete species (ten strains total), two basidiomycete species, and two zygomycete species. We found that growth velocity within a straight channel varied from 1 to 4 μm/min. We also found that the time to complete mazes when fungal hyphae branched or turned at various angles did not correlate with linear growth velocity. We discovered that fungi in our study used one of two distinct strategies to traverse mazes: high-frequency branching in which all possible paths were explored, and low-frequency branching in which only one or two paths were explored. While the high-frequency branching helped fungi escape mazes with sharp turns faster, the low-frequency turning had a significant advantage in mazes with shallower turns. Future work will more systematically examine these trends. Public Library of Science 2021-09-29 /pmc/articles/PMC8480888/ /pubmed/34587206 http://dx.doi.org/10.1371/journal.pone.0257823 Text en © 2021 Hopke et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hopke, Alex Mela, Alex Ellett, Felix Carter-House, Derreck Peña, Jesús F. Stajich, Jason E. Altamirano, Sophie Lovett, Brian Egan, Martin Kale, Shiv Kronholm, Ilkka Guerette, Paul Szewczyk, Edyta McCluskey, Kevin Breslauer, David Shah, Hiral Coad, Bryan R. Momany, Michelle Irimia, Daniel Crowdsourced analysis of fungal growth and branching on microfluidic platforms |
title | Crowdsourced analysis of fungal growth and branching on microfluidic platforms |
title_full | Crowdsourced analysis of fungal growth and branching on microfluidic platforms |
title_fullStr | Crowdsourced analysis of fungal growth and branching on microfluidic platforms |
title_full_unstemmed | Crowdsourced analysis of fungal growth and branching on microfluidic platforms |
title_short | Crowdsourced analysis of fungal growth and branching on microfluidic platforms |
title_sort | crowdsourced analysis of fungal growth and branching on microfluidic platforms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480888/ https://www.ncbi.nlm.nih.gov/pubmed/34587206 http://dx.doi.org/10.1371/journal.pone.0257823 |
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