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Adipose expression of CREB3L3 modulates body weight during obesity
We found the hepatic transcription factor Cyclic-AMP Responsive Element Binding Protein 3-like-3 (CREB3L3) to be expressed in adipose tissue, and selectively downregulated in the more metabolically protective subcutaneous adipose tissue in obese mice and humans. We sought to elucidate the specific r...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481296/ https://www.ncbi.nlm.nih.gov/pubmed/34588527 http://dx.doi.org/10.1038/s41598-021-98627-z |
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author | McCann, Maximilian A. Li, Yanliang Muñoz, Marcos Gil, Victoria Qiang, Guifen Cordoba-Chacon, Jose Blüher, Matthias Duncan, Stephen Liew, Chong Wee |
author_facet | McCann, Maximilian A. Li, Yanliang Muñoz, Marcos Gil, Victoria Qiang, Guifen Cordoba-Chacon, Jose Blüher, Matthias Duncan, Stephen Liew, Chong Wee |
author_sort | McCann, Maximilian A. |
collection | PubMed |
description | We found the hepatic transcription factor Cyclic-AMP Responsive Element Binding Protein 3-like-3 (CREB3L3) to be expressed in adipose tissue, and selectively downregulated in the more metabolically protective subcutaneous adipose tissue in obese mice and humans. We sought to elucidate the specific role of this factor in adipose biology. CREB3L3 fat-specific knockout mice were fed a high-fat diet to induce obesity and metabolic dysfunction. Additionally, we injected a flip-excision adeno-associated virus directly into the subcutaneous inguinal adipose tissue of Adiponectin-Cre mice to create a depot-specific overexpression model for further assessment. Fat-specific ablation of CREB3L3 enhanced weight gain and insulin resistance following high-fat feeding, as fat-specific knockout mice expended less energy and possessed more inflammatory adipose tissue. Conversely, inguinal fat CREB3L3 overexpression deterred diet-induced obesity and ameliorated metabolic dysfunction. Together, this study highlights the relevance of CREB3L3 in obese adipose tissue and demonstrates its role as a powerful body weight modulator. |
format | Online Article Text |
id | pubmed-8481296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84812962021-09-30 Adipose expression of CREB3L3 modulates body weight during obesity McCann, Maximilian A. Li, Yanliang Muñoz, Marcos Gil, Victoria Qiang, Guifen Cordoba-Chacon, Jose Blüher, Matthias Duncan, Stephen Liew, Chong Wee Sci Rep Article We found the hepatic transcription factor Cyclic-AMP Responsive Element Binding Protein 3-like-3 (CREB3L3) to be expressed in adipose tissue, and selectively downregulated in the more metabolically protective subcutaneous adipose tissue in obese mice and humans. We sought to elucidate the specific role of this factor in adipose biology. CREB3L3 fat-specific knockout mice were fed a high-fat diet to induce obesity and metabolic dysfunction. Additionally, we injected a flip-excision adeno-associated virus directly into the subcutaneous inguinal adipose tissue of Adiponectin-Cre mice to create a depot-specific overexpression model for further assessment. Fat-specific ablation of CREB3L3 enhanced weight gain and insulin resistance following high-fat feeding, as fat-specific knockout mice expended less energy and possessed more inflammatory adipose tissue. Conversely, inguinal fat CREB3L3 overexpression deterred diet-induced obesity and ameliorated metabolic dysfunction. Together, this study highlights the relevance of CREB3L3 in obese adipose tissue and demonstrates its role as a powerful body weight modulator. Nature Publishing Group UK 2021-09-29 /pmc/articles/PMC8481296/ /pubmed/34588527 http://dx.doi.org/10.1038/s41598-021-98627-z Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article McCann, Maximilian A. Li, Yanliang Muñoz, Marcos Gil, Victoria Qiang, Guifen Cordoba-Chacon, Jose Blüher, Matthias Duncan, Stephen Liew, Chong Wee Adipose expression of CREB3L3 modulates body weight during obesity |
title | Adipose expression of CREB3L3 modulates body weight during obesity |
title_full | Adipose expression of CREB3L3 modulates body weight during obesity |
title_fullStr | Adipose expression of CREB3L3 modulates body weight during obesity |
title_full_unstemmed | Adipose expression of CREB3L3 modulates body weight during obesity |
title_short | Adipose expression of CREB3L3 modulates body weight during obesity |
title_sort | adipose expression of creb3l3 modulates body weight during obesity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481296/ https://www.ncbi.nlm.nih.gov/pubmed/34588527 http://dx.doi.org/10.1038/s41598-021-98627-z |
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