Cargando…
Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species
Novel molecular platforms are available for identifying (ID) the causative agents of microbial infections and generating antimicrobial susceptibility testing (AST) profiles, which can inform the suitable course of treatment. Many methods claim to perform AST in minutes or hours, often ignoring the n...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481646/ https://www.ncbi.nlm.nih.gov/pubmed/34604191 http://dx.doi.org/10.3389/fbioe.2021.744198 |
_version_ | 1784576721846009856 |
---|---|
author | Chen, Jade Navarro, Eduardo Nuñez, Eliseo Gau, Vincent |
author_facet | Chen, Jade Navarro, Eduardo Nuñez, Eliseo Gau, Vincent |
author_sort | Chen, Jade |
collection | PubMed |
description | Novel molecular platforms are available for identifying (ID) the causative agents of microbial infections and generating antimicrobial susceptibility testing (AST) profiles, which can inform the suitable course of treatment. Many methods claim to perform AST in minutes or hours, often ignoring the need for time-consuming steps such as enrichment cultures and isolation of pure cultures. In clinical microbiology laboratories, an infectious microbial must first be cultured (overnight to days) and identified at the species level, followed by a subsequent AST with an additional turnaround time of 12–48 h due to the need for regrowth of the organism in the absence and presence of relevant antibiotics. Here, we present an electrochemical-based direct-from-specimen ID/AST method for reporting directly from unprocessed urine and blood in hours. In a limit of detection study of 0.5-ml whole blood samples for point-of-care and pediatric applications, 16.7% (4/24) of samples contrived at 2 CFU/ml and 100% (24/24) of samples contrived at 6 CFU/ml were reported positive in 6.5 h, indicating a limit of detection of 6 CFU/ml. In a separate direct-from-specimen AST study, the categorical susceptibility was reported correctly for blinded susceptible, intermediate, resistant, and polymicrobial contrived specimens in 4 h. |
format | Online Article Text |
id | pubmed-8481646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84816462021-10-01 Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species Chen, Jade Navarro, Eduardo Nuñez, Eliseo Gau, Vincent Front Bioeng Biotechnol Bioengineering and Biotechnology Novel molecular platforms are available for identifying (ID) the causative agents of microbial infections and generating antimicrobial susceptibility testing (AST) profiles, which can inform the suitable course of treatment. Many methods claim to perform AST in minutes or hours, often ignoring the need for time-consuming steps such as enrichment cultures and isolation of pure cultures. In clinical microbiology laboratories, an infectious microbial must first be cultured (overnight to days) and identified at the species level, followed by a subsequent AST with an additional turnaround time of 12–48 h due to the need for regrowth of the organism in the absence and presence of relevant antibiotics. Here, we present an electrochemical-based direct-from-specimen ID/AST method for reporting directly from unprocessed urine and blood in hours. In a limit of detection study of 0.5-ml whole blood samples for point-of-care and pediatric applications, 16.7% (4/24) of samples contrived at 2 CFU/ml and 100% (24/24) of samples contrived at 6 CFU/ml were reported positive in 6.5 h, indicating a limit of detection of 6 CFU/ml. In a separate direct-from-specimen AST study, the categorical susceptibility was reported correctly for blinded susceptible, intermediate, resistant, and polymicrobial contrived specimens in 4 h. Frontiers Media S.A. 2021-09-16 /pmc/articles/PMC8481646/ /pubmed/34604191 http://dx.doi.org/10.3389/fbioe.2021.744198 Text en Copyright © 2021 Chen, Navarro, Nuñez and Gau. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Chen, Jade Navarro, Eduardo Nuñez, Eliseo Gau, Vincent Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species |
title | Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species |
title_full | Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species |
title_fullStr | Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species |
title_full_unstemmed | Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species |
title_short | Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species |
title_sort | rapid electrochemical-based pcr-less microbial quantification and antimicrobial susceptibility profiling directly from blood and urine with unknown microbial load or species |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481646/ https://www.ncbi.nlm.nih.gov/pubmed/34604191 http://dx.doi.org/10.3389/fbioe.2021.744198 |
work_keys_str_mv | AT chenjade rapidelectrochemicalbasedpcrlessmicrobialquantificationandantimicrobialsusceptibilityprofilingdirectlyfrombloodandurinewithunknownmicrobialloadorspecies AT navarroeduardo rapidelectrochemicalbasedpcrlessmicrobialquantificationandantimicrobialsusceptibilityprofilingdirectlyfrombloodandurinewithunknownmicrobialloadorspecies AT nunezeliseo rapidelectrochemicalbasedpcrlessmicrobialquantificationandantimicrobialsusceptibilityprofilingdirectlyfrombloodandurinewithunknownmicrobialloadorspecies AT gauvincent rapidelectrochemicalbasedpcrlessmicrobialquantificationandantimicrobialsusceptibilityprofilingdirectlyfrombloodandurinewithunknownmicrobialloadorspecies |