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Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans

To generate a non-red/green fluorescent fusion histone protein in C. elegans, we have generated a C-terminal mTurquoise2-tagged HIS-72 at the endogenous locus using CRISPR. We found that HIS-72::mTurquoise2 localizes in a similar pattern to the previously published HIS-72::GFP strain.

Detalles Bibliográficos
Autores principales:	Sloan, Dillon E., Bembenek, Joshua N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2021
Materias:	Materials and Reagents
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482033/ https://www.ncbi.nlm.nih.gov/pubmed/34604717 http://dx.doi.org/10.17912/micropub.biology.000471

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