Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays

Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques. Isolated HSPCs can be used for do...

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Autores principales: Rodríguez, Alfredo, Filiatrault, Jessica, Flores-Guzmán, Patricia, Mayani, Héctor, Parmar, Kalindi, D’Andrea, Alan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482037/
https://www.ncbi.nlm.nih.gov/pubmed/34622219
http://dx.doi.org/10.1016/j.xpro.2021.100846
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author Rodríguez, Alfredo
Filiatrault, Jessica
Flores-Guzmán, Patricia
Mayani, Héctor
Parmar, Kalindi
D’Andrea, Alan D.
author_facet Rodríguez, Alfredo
Filiatrault, Jessica
Flores-Guzmán, Patricia
Mayani, Héctor
Parmar, Kalindi
D’Andrea, Alan D.
author_sort Rodríguez, Alfredo
collection PubMed
description Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques. Isolated HSPCs can be used for downstream applications, including colony forming unit assays and DNA damage and repair assays. For complete details on the use and execution of this protocol, please refer to Rodríguez et al. (2021a) and (2021b).
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spelling pubmed-84820372021-10-06 Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays Rodríguez, Alfredo Filiatrault, Jessica Flores-Guzmán, Patricia Mayani, Héctor Parmar, Kalindi D’Andrea, Alan D. STAR Protoc Protocol Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow and supply blood cells. Efficient methods for isolation of HSPCs are required. Here, we present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques. Isolated HSPCs can be used for downstream applications, including colony forming unit assays and DNA damage and repair assays. For complete details on the use and execution of this protocol, please refer to Rodríguez et al. (2021a) and (2021b). Elsevier 2021-09-27 /pmc/articles/PMC8482037/ /pubmed/34622219 http://dx.doi.org/10.1016/j.xpro.2021.100846 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rodríguez, Alfredo
Filiatrault, Jessica
Flores-Guzmán, Patricia
Mayani, Héctor
Parmar, Kalindi
D’Andrea, Alan D.
Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
title Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
title_full Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
title_fullStr Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
title_full_unstemmed Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
title_short Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays
title_sort isolation of human and murine hematopoietic stem cells for dna damage and dna repair assays
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482037/
https://www.ncbi.nlm.nih.gov/pubmed/34622219
http://dx.doi.org/10.1016/j.xpro.2021.100846
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