Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord

Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including...

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Detalles Bibliográficos
Autores principales: Dong, Yifei, Lozinski, Brian M., Silva, Claudia, Yong, V. Wee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482042/
https://www.ncbi.nlm.nih.gov/pubmed/34622221
http://dx.doi.org/10.1016/j.xpro.2021.100853
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author Dong, Yifei
Lozinski, Brian M.
Silva, Claudia
Yong, V. Wee
author_facet Dong, Yifei
Lozinski, Brian M.
Silva, Claudia
Yong, V. Wee
author_sort Dong, Yifei
collection PubMed
description Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including cell isolation and culture, animal surgeries, as well as tissue processing and isolation to study the microglia response to OxPC-mediated neurodegeneration in vitro and in vivo. For complete details on the use and execution of this protocol, please refer to Dong et al. (2021).
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spelling pubmed-84820422021-10-06 Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord Dong, Yifei Lozinski, Brian M. Silva, Claudia Yong, V. Wee STAR Protoc Protocol Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including cell isolation and culture, animal surgeries, as well as tissue processing and isolation to study the microglia response to OxPC-mediated neurodegeneration in vitro and in vivo. For complete details on the use and execution of this protocol, please refer to Dong et al. (2021). Elsevier 2021-09-27 /pmc/articles/PMC8482042/ /pubmed/34622221 http://dx.doi.org/10.1016/j.xpro.2021.100853 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Dong, Yifei
Lozinski, Brian M.
Silva, Claudia
Yong, V. Wee
Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
title Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
title_full Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
title_fullStr Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
title_full_unstemmed Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
title_short Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
title_sort studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482042/
https://www.ncbi.nlm.nih.gov/pubmed/34622221
http://dx.doi.org/10.1016/j.xpro.2021.100853
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