Cargando…

Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix

Critically sized defects in subcutaneous white adipose tissue result in extensive disfigurement and dysfunction and remain a reconstructive challenge for surgeons; as larger defect sizes are correlated with higher rates of complications and failure due to insufficient vascularization following impla...

Descripción completa

Detalles Bibliográficos
Autores principales: DeBari, Megan K., Ng, Wai Hoe, Griffin, Mallory D., Kokai, Lauren E., Marra, Kacey G., Rubin, J. Peter, Ren, Xi, Abbott, Rosalyn D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482279/
https://www.ncbi.nlm.nih.gov/pubmed/34562876
http://dx.doi.org/10.3390/biomimetics6030052
_version_ 1784576870965051392
author DeBari, Megan K.
Ng, Wai Hoe
Griffin, Mallory D.
Kokai, Lauren E.
Marra, Kacey G.
Rubin, J. Peter
Ren, Xi
Abbott, Rosalyn D.
author_facet DeBari, Megan K.
Ng, Wai Hoe
Griffin, Mallory D.
Kokai, Lauren E.
Marra, Kacey G.
Rubin, J. Peter
Ren, Xi
Abbott, Rosalyn D.
author_sort DeBari, Megan K.
collection PubMed
description Critically sized defects in subcutaneous white adipose tissue result in extensive disfigurement and dysfunction and remain a reconstructive challenge for surgeons; as larger defect sizes are correlated with higher rates of complications and failure due to insufficient vascularization following implantation. Our study demonstrates, for the first time, a method to engineer perfusable, pre-vascularized, high-density adipose grafts that combine patient-derived adipose cells with a decellularized lung matrix (DLM). The lung is one of the most vascularized organs with high flow, low resistance, and a large blood–alveolar interface separated by a thin basement membrane. For our work, the large volume capacity within the alveolar compartment was repurposed for high-density adipose cell filling, while the acellular vascular bed provided efficient graft perfusion throughout. Both adipocytes and hASCs were successfully delivered and remained in the alveolar space even after weeks of culture. While adipose-derived cells maintained their morphology and functionality in both static and perfusion DLM cultures, perfusion culture offered enhanced outcomes over static culture. Furthermore, we demonstrate that endothelial cells seamlessly integrate into the acellular vascular tree of the DLM with adipocytes. These results support that the DLM is a unique platform for creating vascularized adipose tissue grafts for large defect filling.
format Online
Article
Text
id pubmed-8482279
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84822792021-10-01 Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix DeBari, Megan K. Ng, Wai Hoe Griffin, Mallory D. Kokai, Lauren E. Marra, Kacey G. Rubin, J. Peter Ren, Xi Abbott, Rosalyn D. Biomimetics (Basel) Article Critically sized defects in subcutaneous white adipose tissue result in extensive disfigurement and dysfunction and remain a reconstructive challenge for surgeons; as larger defect sizes are correlated with higher rates of complications and failure due to insufficient vascularization following implantation. Our study demonstrates, for the first time, a method to engineer perfusable, pre-vascularized, high-density adipose grafts that combine patient-derived adipose cells with a decellularized lung matrix (DLM). The lung is one of the most vascularized organs with high flow, low resistance, and a large blood–alveolar interface separated by a thin basement membrane. For our work, the large volume capacity within the alveolar compartment was repurposed for high-density adipose cell filling, while the acellular vascular bed provided efficient graft perfusion throughout. Both adipocytes and hASCs were successfully delivered and remained in the alveolar space even after weeks of culture. While adipose-derived cells maintained their morphology and functionality in both static and perfusion DLM cultures, perfusion culture offered enhanced outcomes over static culture. Furthermore, we demonstrate that endothelial cells seamlessly integrate into the acellular vascular tree of the DLM with adipocytes. These results support that the DLM is a unique platform for creating vascularized adipose tissue grafts for large defect filling. MDPI 2021-09-18 /pmc/articles/PMC8482279/ /pubmed/34562876 http://dx.doi.org/10.3390/biomimetics6030052 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
DeBari, Megan K.
Ng, Wai Hoe
Griffin, Mallory D.
Kokai, Lauren E.
Marra, Kacey G.
Rubin, J. Peter
Ren, Xi
Abbott, Rosalyn D.
Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix
title Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix
title_full Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix
title_fullStr Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix
title_full_unstemmed Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix
title_short Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix
title_sort engineering a 3d vascularized adipose tissue construct using a decellularized lung matrix
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482279/
https://www.ncbi.nlm.nih.gov/pubmed/34562876
http://dx.doi.org/10.3390/biomimetics6030052
work_keys_str_mv AT debarimegank engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT ngwaihoe engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT griffinmalloryd engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT kokailaurene engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT marrakaceyg engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT rubinjpeter engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT renxi engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix
AT abbottrosalynd engineeringa3dvascularizedadiposetissueconstructusingadecellularizedlungmatrix