The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16

Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were cha...

Descripción completa

Detalles Bibliográficos
Autores principales: Ehsaan, Muhammad, Baker, Jonathan, Kovács, Katalin, Malys, Naglis, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Biomedical 2021
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482281/
https://www.ncbi.nlm.nih.gov/pubmed/34506812
http://dx.doi.org/10.1016/j.mimet.2021.106323
Descripción
Sumario:Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility.

Ejemplares similares