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The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were cha...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Biomedical
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482281/ https://www.ncbi.nlm.nih.gov/pubmed/34506812 http://dx.doi.org/10.1016/j.mimet.2021.106323 |
Sumario: | Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility. |
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