The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16

Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were cha...

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Detalles Bibliográficos
Autores principales: Ehsaan, Muhammad, Baker, Jonathan, Kovács, Katalin, Malys, Naglis, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Biomedical 2021
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482281/
https://www.ncbi.nlm.nih.gov/pubmed/34506812
http://dx.doi.org/10.1016/j.mimet.2021.106323
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author Ehsaan, Muhammad
Baker, Jonathan
Kovács, Katalin
Malys, Naglis
Minton, Nigel P.
author_facet Ehsaan, Muhammad
Baker, Jonathan
Kovács, Katalin
Malys, Naglis
Minton, Nigel P.
author_sort Ehsaan, Muhammad
collection PubMed
description Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility.
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spelling pubmed-84822812021-10-06 The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16 Ehsaan, Muhammad Baker, Jonathan Kovács, Katalin Malys, Naglis Minton, Nigel P. J Microbiol Methods Note Cupriavidus necator H16 can convert CO(2) into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility. Elsevier Biomedical 2021-10 /pmc/articles/PMC8482281/ /pubmed/34506812 http://dx.doi.org/10.1016/j.mimet.2021.106323 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Note
Ehsaan, Muhammad
Baker, Jonathan
Kovács, Katalin
Malys, Naglis
Minton, Nigel P.
The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
title The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
title_full The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
title_fullStr The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
title_full_unstemmed The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
title_short The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
title_sort pmtl70000 modular, plasmid vector series for strain engineering in cupriavidus necator h16
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482281/
https://www.ncbi.nlm.nih.gov/pubmed/34506812
http://dx.doi.org/10.1016/j.mimet.2021.106323
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