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Comprehensive Profiling of Macamides and Fatty Acid Derivatives in Maca with Different Postharvest Drying Processes Using UPLC-QTOF-MS
[Image: see text] Macamides are characteristically found in maca (Lepidium meyenii Walper). Fatty acid derivatives are also an important type of constituent in maca, since they not only relate to the biosynthesis of macamides in the postharvest process but also possess some bioactivities. To study t...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482404/ https://www.ncbi.nlm.nih.gov/pubmed/34604630 http://dx.doi.org/10.1021/acsomega.1c02926 |
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author | Xia, Chen Deng, Junlin Pan, Yu Lin, Changbin Zhu, Yongqing Xiang, Zhuoya Li, Wanyi Chen, Jian Zhang, Yingjiao Zhu, Boyu Huang, Qiaolian |
author_facet | Xia, Chen Deng, Junlin Pan, Yu Lin, Changbin Zhu, Yongqing Xiang, Zhuoya Li, Wanyi Chen, Jian Zhang, Yingjiao Zhu, Boyu Huang, Qiaolian |
author_sort | Xia, Chen |
collection | PubMed |
description | [Image: see text] Macamides are characteristically found in maca (Lepidium meyenii Walper). Fatty acid derivatives are also an important type of constituent in maca, since they not only relate to the biosynthesis of macamides in the postharvest process but also possess some bioactivities. To study their comprehensive profiles in maca tubers processed via the air-drying method, ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) analyses were performed to identify macamide and fatty acid molecules. Their contents in maca tubers that were processed via air drying and freeze drying, respectively, were further quantified using high-performance liquid chromatography (HPLC) analyses comparing with eight macamide and three fatty acid reference standards. A total of 19 macamides (including four novel ones) and 16 fatty acid derivatives (two found in maca for the first time) were identified. Quantification analyses results showed the eight macamides with contents ranging from 31.39 to 1163.19 μg/g (on dry tuber), and fatty acids from 18.71 to 181.99 μg/g in the air-dried maca, but there were only three macamides and one fatty acid detected with very low contents (3.97–34.36 μg/g) in the freeze-dried maca. The results demonstrated that the air-drying method can increase the accumulations of macamides and fatty acids in the metabolism of maca in the postharvest process. The biosynthesis of two types of macamides, i.e., N-benzyl-oxo-octadecadienamides and N-benzyl-oxo-octadecatrienamides, was further elucidated in detail. These results provide more valuable insights into the phytochemicals of maca, which is helpful to explain its health benefits. |
format | Online Article Text |
id | pubmed-8482404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-84824042021-10-01 Comprehensive Profiling of Macamides and Fatty Acid Derivatives in Maca with Different Postharvest Drying Processes Using UPLC-QTOF-MS Xia, Chen Deng, Junlin Pan, Yu Lin, Changbin Zhu, Yongqing Xiang, Zhuoya Li, Wanyi Chen, Jian Zhang, Yingjiao Zhu, Boyu Huang, Qiaolian ACS Omega [Image: see text] Macamides are characteristically found in maca (Lepidium meyenii Walper). Fatty acid derivatives are also an important type of constituent in maca, since they not only relate to the biosynthesis of macamides in the postharvest process but also possess some bioactivities. To study their comprehensive profiles in maca tubers processed via the air-drying method, ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) analyses were performed to identify macamide and fatty acid molecules. Their contents in maca tubers that were processed via air drying and freeze drying, respectively, were further quantified using high-performance liquid chromatography (HPLC) analyses comparing with eight macamide and three fatty acid reference standards. A total of 19 macamides (including four novel ones) and 16 fatty acid derivatives (two found in maca for the first time) were identified. Quantification analyses results showed the eight macamides with contents ranging from 31.39 to 1163.19 μg/g (on dry tuber), and fatty acids from 18.71 to 181.99 μg/g in the air-dried maca, but there were only three macamides and one fatty acid detected with very low contents (3.97–34.36 μg/g) in the freeze-dried maca. The results demonstrated that the air-drying method can increase the accumulations of macamides and fatty acids in the metabolism of maca in the postharvest process. The biosynthesis of two types of macamides, i.e., N-benzyl-oxo-octadecadienamides and N-benzyl-oxo-octadecatrienamides, was further elucidated in detail. These results provide more valuable insights into the phytochemicals of maca, which is helpful to explain its health benefits. American Chemical Society 2021-09-18 /pmc/articles/PMC8482404/ /pubmed/34604630 http://dx.doi.org/10.1021/acsomega.1c02926 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Xia, Chen Deng, Junlin Pan, Yu Lin, Changbin Zhu, Yongqing Xiang, Zhuoya Li, Wanyi Chen, Jian Zhang, Yingjiao Zhu, Boyu Huang, Qiaolian Comprehensive Profiling of Macamides and Fatty Acid Derivatives in Maca with Different Postharvest Drying Processes Using UPLC-QTOF-MS |
title | Comprehensive Profiling of Macamides and Fatty Acid
Derivatives in Maca with Different Postharvest Drying Processes Using
UPLC-QTOF-MS |
title_full | Comprehensive Profiling of Macamides and Fatty Acid
Derivatives in Maca with Different Postharvest Drying Processes Using
UPLC-QTOF-MS |
title_fullStr | Comprehensive Profiling of Macamides and Fatty Acid
Derivatives in Maca with Different Postharvest Drying Processes Using
UPLC-QTOF-MS |
title_full_unstemmed | Comprehensive Profiling of Macamides and Fatty Acid
Derivatives in Maca with Different Postharvest Drying Processes Using
UPLC-QTOF-MS |
title_short | Comprehensive Profiling of Macamides and Fatty Acid
Derivatives in Maca with Different Postharvest Drying Processes Using
UPLC-QTOF-MS |
title_sort | comprehensive profiling of macamides and fatty acid
derivatives in maca with different postharvest drying processes using
uplc-qtof-ms |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482404/ https://www.ncbi.nlm.nih.gov/pubmed/34604630 http://dx.doi.org/10.1021/acsomega.1c02926 |
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