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Involvement of RNA granule proteins in meiotic silencing by unpaired DNA

In Neurospora crassa, expression from an unpaired gene is suppressed by a mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) factors to silence target mRNAs. Here, we report that Neurospora CAR-1 and CGH-1, homologs of two Caenorhabditis elegans...

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Autores principales: Xiao, Hua, Vierling, Michael M, Kennedy, Rana F, Boone, Erin C, Decker, Logan M, Sy, Victor T, Haynes, Jackson B, Williams, Michelle A, Shiu, Patrick K T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482848/
https://www.ncbi.nlm.nih.gov/pubmed/34568932
http://dx.doi.org/10.1093/g3journal/jkab179
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author Xiao, Hua
Vierling, Michael M
Kennedy, Rana F
Boone, Erin C
Decker, Logan M
Sy, Victor T
Haynes, Jackson B
Williams, Michelle A
Shiu, Patrick K T
author_facet Xiao, Hua
Vierling, Michael M
Kennedy, Rana F
Boone, Erin C
Decker, Logan M
Sy, Victor T
Haynes, Jackson B
Williams, Michelle A
Shiu, Patrick K T
author_sort Xiao, Hua
collection PubMed
description In Neurospora crassa, expression from an unpaired gene is suppressed by a mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) factors to silence target mRNAs. Here, we report that Neurospora CAR-1 and CGH-1, homologs of two Caenorhabditis elegans RNA granule components, are involved in MSUD. These fungal proteins are found in the perinuclear region and P-bodies, much like their worm counterparts. They interact with components of the meiotic silencing complex (MSC), including the SMS-2 Argonaute. This is the first time MSUD has been linked to RNA granule proteins.
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spelling pubmed-84828482021-10-01 Involvement of RNA granule proteins in meiotic silencing by unpaired DNA Xiao, Hua Vierling, Michael M Kennedy, Rana F Boone, Erin C Decker, Logan M Sy, Victor T Haynes, Jackson B Williams, Michelle A Shiu, Patrick K T G3 (Bethesda) Investigation In Neurospora crassa, expression from an unpaired gene is suppressed by a mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) factors to silence target mRNAs. Here, we report that Neurospora CAR-1 and CGH-1, homologs of two Caenorhabditis elegans RNA granule components, are involved in MSUD. These fungal proteins are found in the perinuclear region and P-bodies, much like their worm counterparts. They interact with components of the meiotic silencing complex (MSC), including the SMS-2 Argonaute. This is the first time MSUD has been linked to RNA granule proteins. Oxford University Press 2021-05-13 /pmc/articles/PMC8482848/ /pubmed/34568932 http://dx.doi.org/10.1093/g3journal/jkab179 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Xiao, Hua
Vierling, Michael M
Kennedy, Rana F
Boone, Erin C
Decker, Logan M
Sy, Victor T
Haynes, Jackson B
Williams, Michelle A
Shiu, Patrick K T
Involvement of RNA granule proteins in meiotic silencing by unpaired DNA
title Involvement of RNA granule proteins in meiotic silencing by unpaired DNA
title_full Involvement of RNA granule proteins in meiotic silencing by unpaired DNA
title_fullStr Involvement of RNA granule proteins in meiotic silencing by unpaired DNA
title_full_unstemmed Involvement of RNA granule proteins in meiotic silencing by unpaired DNA
title_short Involvement of RNA granule proteins in meiotic silencing by unpaired DNA
title_sort involvement of rna granule proteins in meiotic silencing by unpaired dna
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482848/
https://www.ncbi.nlm.nih.gov/pubmed/34568932
http://dx.doi.org/10.1093/g3journal/jkab179
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