Cargando…
Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis
BACKGROUND: Hepatocellular carcinoma (HCC) is the third cancer-related cause of death in the world. Until now, the involved mechanisms during the development of HCC are largely unknown. This study aims to explore the driven genes and potential drugs in HCC. METHODS: Three mRNA expression datasets we...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483840/ https://www.ncbi.nlm.nih.gov/pubmed/34596112 http://dx.doi.org/10.1097/MD.0000000000027117 |
_version_ | 1784577198674411520 |
---|---|
author | Chen, Xiaolong Xia, Zhixiong Wan, Yafeng Huang, Ping |
author_facet | Chen, Xiaolong Xia, Zhixiong Wan, Yafeng Huang, Ping |
author_sort | Chen, Xiaolong |
collection | PubMed |
description | BACKGROUND: Hepatocellular carcinoma (HCC) is the third cancer-related cause of death in the world. Until now, the involved mechanisms during the development of HCC are largely unknown. This study aims to explore the driven genes and potential drugs in HCC. METHODS: Three mRNA expression datasets were used to analyze the differentially expressed genes (DEGs) in HCC. The bioinformatics approaches include identification of DEGs and hub genes, Gene Ontology terms analysis and Kyoto encyclopedia of genes and genomes enrichment analysis, construction of protein–protein interaction network. The expression levels of hub genes were validated based on The Cancer Genome Atlas, Gene Expression Profiling Interactive Analysis, and the Human Protein Atlas. Moreover, overall survival and disease-free survival analysis of HCC patients were further conducted by Kaplan–Meier plotter and Gene Expression Profiling Interactive Analysis. DGIdb database was performed to search the candidate drugs for HCC. RESULTS: A total of 197 DEGs were identified. The protein–protein interaction network was constructed using Search Tool for the Retrieval of Interacting Genes software, 10 genes were selected by Cytoscape plugin cytoHubba and served as hub genes. These 10 genes were all closely related to the survival of HCC patients. DGIdb database predicted 29 small molecules as the possible drugs for treating HCC. CONCLUSION: Our study provides some new insights into HCC pathogenesis and treatments. The candidate drugs may improve the efficiency of HCC therapy in the future. |
format | Online Article Text |
id | pubmed-8483840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-84838402021-10-04 Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis Chen, Xiaolong Xia, Zhixiong Wan, Yafeng Huang, Ping Medicine (Baltimore) 4500 BACKGROUND: Hepatocellular carcinoma (HCC) is the third cancer-related cause of death in the world. Until now, the involved mechanisms during the development of HCC are largely unknown. This study aims to explore the driven genes and potential drugs in HCC. METHODS: Three mRNA expression datasets were used to analyze the differentially expressed genes (DEGs) in HCC. The bioinformatics approaches include identification of DEGs and hub genes, Gene Ontology terms analysis and Kyoto encyclopedia of genes and genomes enrichment analysis, construction of protein–protein interaction network. The expression levels of hub genes were validated based on The Cancer Genome Atlas, Gene Expression Profiling Interactive Analysis, and the Human Protein Atlas. Moreover, overall survival and disease-free survival analysis of HCC patients were further conducted by Kaplan–Meier plotter and Gene Expression Profiling Interactive Analysis. DGIdb database was performed to search the candidate drugs for HCC. RESULTS: A total of 197 DEGs were identified. The protein–protein interaction network was constructed using Search Tool for the Retrieval of Interacting Genes software, 10 genes were selected by Cytoscape plugin cytoHubba and served as hub genes. These 10 genes were all closely related to the survival of HCC patients. DGIdb database predicted 29 small molecules as the possible drugs for treating HCC. CONCLUSION: Our study provides some new insights into HCC pathogenesis and treatments. The candidate drugs may improve the efficiency of HCC therapy in the future. Lippincott Williams & Wilkins 2021-10-01 /pmc/articles/PMC8483840/ /pubmed/34596112 http://dx.doi.org/10.1097/MD.0000000000027117 Text en Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) |
spellingShingle | 4500 Chen, Xiaolong Xia, Zhixiong Wan, Yafeng Huang, Ping Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
title | Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
title_full | Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
title_fullStr | Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
title_full_unstemmed | Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
title_short | Identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
title_sort | identification of hub genes and candidate drugs in hepatocellular carcinoma by integrated bioinformatics analysis |
topic | 4500 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483840/ https://www.ncbi.nlm.nih.gov/pubmed/34596112 http://dx.doi.org/10.1097/MD.0000000000027117 |
work_keys_str_mv | AT chenxiaolong identificationofhubgenesandcandidatedrugsinhepatocellularcarcinomabyintegratedbioinformaticsanalysis AT xiazhixiong identificationofhubgenesandcandidatedrugsinhepatocellularcarcinomabyintegratedbioinformaticsanalysis AT wanyafeng identificationofhubgenesandcandidatedrugsinhepatocellularcarcinomabyintegratedbioinformaticsanalysis AT huangping identificationofhubgenesandcandidatedrugsinhepatocellularcarcinomabyintegratedbioinformaticsanalysis |