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Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro

Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threat worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Sp...

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Autores principales: Wang, Gang, Guan, Jun, Li, Guojun, Wu, Fengtian, Yang, Qin, Huang, Chunhong, Shao, Junwei, Xu, Lichen, Guo, Zixuan, Zhou, Qihui, Zhu, Haihong, Chen, Zhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483903/
https://www.ncbi.nlm.nih.gov/pubmed/34603560
http://dx.doi.org/10.1155/2021/6803510
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author Wang, Gang
Guan, Jun
Li, Guojun
Wu, Fengtian
Yang, Qin
Huang, Chunhong
Shao, Junwei
Xu, Lichen
Guo, Zixuan
Zhou, Qihui
Zhu, Haihong
Chen, Zhi
author_facet Wang, Gang
Guan, Jun
Li, Guojun
Wu, Fengtian
Yang, Qin
Huang, Chunhong
Shao, Junwei
Xu, Lichen
Guo, Zixuan
Zhou, Qihui
Zhu, Haihong
Chen, Zhi
author_sort Wang, Gang
collection PubMed
description Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threat worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Specific components of the SARS-CoV-2 virus trigger lung inflammation, and recruitment of immune cells to the lungs exacerbates this process, although much remains unknown about the pathogenesis of COVID-19. Our study of lung type II pneumocyte cells (A549) demonstrated that ORF7, an open reading frame (ORF) in the genome of SARS-CoV-2, induced the production of CCL2, a chemokine that promotes the chemotaxis of monocytes, and decreased the expression of IL-8, a chemokine that recruits neutrophils. A549 cells also had an increased level of IL-6. The results of our chemotaxis Transwell assay suggested that ORF7 augmented monocyte infiltration and reduced the number of neutrophils. We conclude that the ORF7 of SARS-CoV-2 may have specific effects on the immunological changes in tissues after infection. These results suggest that the functions of other ORFs of SARS-CoV-2 should also be comprehensively examined.
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spelling pubmed-84839032021-10-01 Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro Wang, Gang Guan, Jun Li, Guojun Wu, Fengtian Yang, Qin Huang, Chunhong Shao, Junwei Xu, Lichen Guo, Zixuan Zhou, Qihui Zhu, Haihong Chen, Zhi Dis Markers Research Article Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threat worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Specific components of the SARS-CoV-2 virus trigger lung inflammation, and recruitment of immune cells to the lungs exacerbates this process, although much remains unknown about the pathogenesis of COVID-19. Our study of lung type II pneumocyte cells (A549) demonstrated that ORF7, an open reading frame (ORF) in the genome of SARS-CoV-2, induced the production of CCL2, a chemokine that promotes the chemotaxis of monocytes, and decreased the expression of IL-8, a chemokine that recruits neutrophils. A549 cells also had an increased level of IL-6. The results of our chemotaxis Transwell assay suggested that ORF7 augmented monocyte infiltration and reduced the number of neutrophils. We conclude that the ORF7 of SARS-CoV-2 may have specific effects on the immunological changes in tissues after infection. These results suggest that the functions of other ORFs of SARS-CoV-2 should also be comprehensively examined. Hindawi 2021-09-28 /pmc/articles/PMC8483903/ /pubmed/34603560 http://dx.doi.org/10.1155/2021/6803510 Text en Copyright © 2021 Gang Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Gang
Guan, Jun
Li, Guojun
Wu, Fengtian
Yang, Qin
Huang, Chunhong
Shao, Junwei
Xu, Lichen
Guo, Zixuan
Zhou, Qihui
Zhu, Haihong
Chen, Zhi
Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro
title Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro
title_full Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro
title_fullStr Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro
title_full_unstemmed Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro
title_short Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro
title_sort effect of orf7 of sars-cov-2 on the chemotaxis of monocytes and neutrophils in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483903/
https://www.ncbi.nlm.nih.gov/pubmed/34603560
http://dx.doi.org/10.1155/2021/6803510
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