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Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice

BACKGROUND: Stress ulcer (SU) is a serious gastrointestinal mucosal lesion under acute stress. Huanglian decoction is a famous traditional Chinese medicine prescription, which has been used to treat digestive system diseases for thousands of years. Many clinical cases have proved that Huanglian deco...

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Autores principales: Zhang, Qi, Guo, Jing-Jing, Yau, Yuen-Ming, Wang, Ying-Jie, Cheng, Yan-Bin, Tuo, Xuan, Yang, Zong-Bao, Qian, Lin-Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483906/
https://www.ncbi.nlm.nih.gov/pubmed/34603467
http://dx.doi.org/10.1155/2021/3087270
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author Zhang, Qi
Guo, Jing-Jing
Yau, Yuen-Ming
Wang, Ying-Jie
Cheng, Yan-Bin
Tuo, Xuan
Yang, Zong-Bao
Qian, Lin-Chao
author_facet Zhang, Qi
Guo, Jing-Jing
Yau, Yuen-Ming
Wang, Ying-Jie
Cheng, Yan-Bin
Tuo, Xuan
Yang, Zong-Bao
Qian, Lin-Chao
author_sort Zhang, Qi
collection PubMed
description BACKGROUND: Stress ulcer (SU) is a serious gastrointestinal mucosal lesion under acute stress. Huanglian decoction is a famous traditional Chinese medicine prescription, which has been used to treat digestive system diseases for thousands of years. Many clinical cases have proved that Huanglian decoction has a good effect on SU. Some studies have shown that the intestinal bacteria will be changed accordingly when the gastrointestinal mucosa is damaged. However, there are few published reports on the effect of the intestinal microbiome with SU mice that were treated by Huanglian decoction. In this study, we investigated the effect of the fecal microbiome in mice with SU by the 16S rDNA sequencing technology. METHODS: Male KM mice were induced by cold-restraint stress except for the normal control group and then treated by Huanglian decoction (Group HD) and Esomeprazole magnesium solution (Group ES) separately for 7 days. 16S rDNA sequencing technology analysis was applied to evaluate the changes of bacterial flora in mice feces. And, histopathological methods and molecular biological detection methods were also performed. RESULTS: Huanglian decoction could help to repair the gastric mucosal injury and regulate the relative content of TNF-α and IL-1β. Moreover, Huanglian decoction could increase the relative abundance of intestinal probiotics in the intestine of mice with SU, especially in Verrucomicrobiae and Akkermansia. CONCLUSIONS: Huanglian decoction might effectively promote the repair of gastrointestinal mucosal injury and regulate the number and species of intestinal bacteria to maintain the stability of gastrointestinal microecology.
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spelling pubmed-84839062021-10-01 Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice Zhang, Qi Guo, Jing-Jing Yau, Yuen-Ming Wang, Ying-Jie Cheng, Yan-Bin Tuo, Xuan Yang, Zong-Bao Qian, Lin-Chao Evid Based Complement Alternat Med Research Article BACKGROUND: Stress ulcer (SU) is a serious gastrointestinal mucosal lesion under acute stress. Huanglian decoction is a famous traditional Chinese medicine prescription, which has been used to treat digestive system diseases for thousands of years. Many clinical cases have proved that Huanglian decoction has a good effect on SU. Some studies have shown that the intestinal bacteria will be changed accordingly when the gastrointestinal mucosa is damaged. However, there are few published reports on the effect of the intestinal microbiome with SU mice that were treated by Huanglian decoction. In this study, we investigated the effect of the fecal microbiome in mice with SU by the 16S rDNA sequencing technology. METHODS: Male KM mice were induced by cold-restraint stress except for the normal control group and then treated by Huanglian decoction (Group HD) and Esomeprazole magnesium solution (Group ES) separately for 7 days. 16S rDNA sequencing technology analysis was applied to evaluate the changes of bacterial flora in mice feces. And, histopathological methods and molecular biological detection methods were also performed. RESULTS: Huanglian decoction could help to repair the gastric mucosal injury and regulate the relative content of TNF-α and IL-1β. Moreover, Huanglian decoction could increase the relative abundance of intestinal probiotics in the intestine of mice with SU, especially in Verrucomicrobiae and Akkermansia. CONCLUSIONS: Huanglian decoction might effectively promote the repair of gastrointestinal mucosal injury and regulate the number and species of intestinal bacteria to maintain the stability of gastrointestinal microecology. Hindawi 2021-09-22 /pmc/articles/PMC8483906/ /pubmed/34603467 http://dx.doi.org/10.1155/2021/3087270 Text en Copyright © 2021 Qi Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Qi
Guo, Jing-Jing
Yau, Yuen-Ming
Wang, Ying-Jie
Cheng, Yan-Bin
Tuo, Xuan
Yang, Zong-Bao
Qian, Lin-Chao
Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice
title Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice
title_full Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice
title_fullStr Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice
title_full_unstemmed Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice
title_short Effect of Huanglian Decoction on the Intestinal Microbiome in Stress Ulcer (SU) Mice
title_sort effect of huanglian decoction on the intestinal microbiome in stress ulcer (su) mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483906/
https://www.ncbi.nlm.nih.gov/pubmed/34603467
http://dx.doi.org/10.1155/2021/3087270
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