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Zinc oxide nanoparticles promote the aging process in a size-dependent manner
Zinc oxide (ZnO) nanoparticles (NPs) are generally utilized in cosmetic goods, sheds, biosensors, and delivery of drug. As in vitro ideal systems, mesenchymal stem cells (MSCs) are used to test acute toxicity. In the present study, size-dependent cytotoxicity effects of ZnO NPs on MSCs were assessed...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484102/ https://www.ncbi.nlm.nih.gov/pubmed/34591206 http://dx.doi.org/10.1007/s10856-021-06602-x |
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author | Deylam, Mahla Alizadeh, Effat Sarikhani, Manizheh Hejazy, Marzie Firouzamandi, Masoumeh |
author_facet | Deylam, Mahla Alizadeh, Effat Sarikhani, Manizheh Hejazy, Marzie Firouzamandi, Masoumeh |
author_sort | Deylam, Mahla |
collection | PubMed |
description | Zinc oxide (ZnO) nanoparticles (NPs) are generally utilized in cosmetic goods, sheds, biosensors, and delivery of drug. As in vitro ideal systems, mesenchymal stem cells (MSCs) are used to test acute toxicity. In the present study, size-dependent cytotoxicity effects of ZnO NPs on MSCs were assessed. Bone marrow and adipose MSCs were treated with ZnO NPs with average sizes of 10–30 and 35–45 nm. The 5 and 10 µg/ml concentrations of ZnO NP were found to be the safe concentrations for the NP sizes of 10–30 and 35–45 nm, respectively. Cell-cycle analysis indicated that the small size of ZnO NPs has more negative effects on the process of cell entry to DNA synthesis when compared to the larger size. The results of the β-galactosidase test showed the promotion of the aging process in the cells treated with the smaller size of ZnO NPs. Both sizes of the NP were found to upregulate the aging-related genes NF-kB and p53 and downregulate the anti-aging gene Nanog. To sum up, the smaller size of ZnO NPs can enhance the aging process in the cells. [Image: see text] |
format | Online Article Text |
id | pubmed-8484102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-84841022021-10-08 Zinc oxide nanoparticles promote the aging process in a size-dependent manner Deylam, Mahla Alizadeh, Effat Sarikhani, Manizheh Hejazy, Marzie Firouzamandi, Masoumeh J Mater Sci Mater Med Biomaterials Synthesis and Characterization Zinc oxide (ZnO) nanoparticles (NPs) are generally utilized in cosmetic goods, sheds, biosensors, and delivery of drug. As in vitro ideal systems, mesenchymal stem cells (MSCs) are used to test acute toxicity. In the present study, size-dependent cytotoxicity effects of ZnO NPs on MSCs were assessed. Bone marrow and adipose MSCs were treated with ZnO NPs with average sizes of 10–30 and 35–45 nm. The 5 and 10 µg/ml concentrations of ZnO NP were found to be the safe concentrations for the NP sizes of 10–30 and 35–45 nm, respectively. Cell-cycle analysis indicated that the small size of ZnO NPs has more negative effects on the process of cell entry to DNA synthesis when compared to the larger size. The results of the β-galactosidase test showed the promotion of the aging process in the cells treated with the smaller size of ZnO NPs. Both sizes of the NP were found to upregulate the aging-related genes NF-kB and p53 and downregulate the anti-aging gene Nanog. To sum up, the smaller size of ZnO NPs can enhance the aging process in the cells. [Image: see text] Springer US 2021-09-30 2021 /pmc/articles/PMC8484102/ /pubmed/34591206 http://dx.doi.org/10.1007/s10856-021-06602-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biomaterials Synthesis and Characterization Deylam, Mahla Alizadeh, Effat Sarikhani, Manizheh Hejazy, Marzie Firouzamandi, Masoumeh Zinc oxide nanoparticles promote the aging process in a size-dependent manner |
title | Zinc oxide nanoparticles promote the aging process in a size-dependent manner |
title_full | Zinc oxide nanoparticles promote the aging process in a size-dependent manner |
title_fullStr | Zinc oxide nanoparticles promote the aging process in a size-dependent manner |
title_full_unstemmed | Zinc oxide nanoparticles promote the aging process in a size-dependent manner |
title_short | Zinc oxide nanoparticles promote the aging process in a size-dependent manner |
title_sort | zinc oxide nanoparticles promote the aging process in a size-dependent manner |
topic | Biomaterials Synthesis and Characterization |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484102/ https://www.ncbi.nlm.nih.gov/pubmed/34591206 http://dx.doi.org/10.1007/s10856-021-06602-x |
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