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Zinc oxide nanoparticles promote the aging process in a size-dependent manner

Zinc oxide (ZnO) nanoparticles (NPs) are generally utilized in cosmetic goods, sheds, biosensors, and delivery of drug. As in vitro ideal systems, mesenchymal stem cells (MSCs) are used to test acute toxicity. In the present study, size-dependent cytotoxicity effects of ZnO NPs on MSCs were assessed...

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Autores principales: Deylam, Mahla, Alizadeh, Effat, Sarikhani, Manizheh, Hejazy, Marzie, Firouzamandi, Masoumeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484102/
https://www.ncbi.nlm.nih.gov/pubmed/34591206
http://dx.doi.org/10.1007/s10856-021-06602-x
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author Deylam, Mahla
Alizadeh, Effat
Sarikhani, Manizheh
Hejazy, Marzie
Firouzamandi, Masoumeh
author_facet Deylam, Mahla
Alizadeh, Effat
Sarikhani, Manizheh
Hejazy, Marzie
Firouzamandi, Masoumeh
author_sort Deylam, Mahla
collection PubMed
description Zinc oxide (ZnO) nanoparticles (NPs) are generally utilized in cosmetic goods, sheds, biosensors, and delivery of drug. As in vitro ideal systems, mesenchymal stem cells (MSCs) are used to test acute toxicity. In the present study, size-dependent cytotoxicity effects of ZnO NPs on MSCs were assessed. Bone marrow and adipose MSCs were treated with ZnO NPs with average sizes of 10–30 and 35–45 nm. The 5 and 10 µg/ml concentrations of ZnO NP were found to be the safe concentrations for the NP sizes of 10–30 and 35–45 nm, respectively. Cell-cycle analysis indicated that the small size of ZnO NPs has more negative effects on the process of cell entry to DNA synthesis when compared to the larger size. The results of the β-galactosidase test showed the promotion of the aging process in the cells treated with the smaller size of ZnO NPs. Both sizes of the NP were found to upregulate the aging-related genes NF-kB and p53 and downregulate the anti-aging gene Nanog. To sum up, the smaller size of ZnO NPs can enhance the aging process in the cells. [Image: see text]
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spelling pubmed-84841022021-10-08 Zinc oxide nanoparticles promote the aging process in a size-dependent manner Deylam, Mahla Alizadeh, Effat Sarikhani, Manizheh Hejazy, Marzie Firouzamandi, Masoumeh J Mater Sci Mater Med Biomaterials Synthesis and Characterization Zinc oxide (ZnO) nanoparticles (NPs) are generally utilized in cosmetic goods, sheds, biosensors, and delivery of drug. As in vitro ideal systems, mesenchymal stem cells (MSCs) are used to test acute toxicity. In the present study, size-dependent cytotoxicity effects of ZnO NPs on MSCs were assessed. Bone marrow and adipose MSCs were treated with ZnO NPs with average sizes of 10–30 and 35–45 nm. The 5 and 10 µg/ml concentrations of ZnO NP were found to be the safe concentrations for the NP sizes of 10–30 and 35–45 nm, respectively. Cell-cycle analysis indicated that the small size of ZnO NPs has more negative effects on the process of cell entry to DNA synthesis when compared to the larger size. The results of the β-galactosidase test showed the promotion of the aging process in the cells treated with the smaller size of ZnO NPs. Both sizes of the NP were found to upregulate the aging-related genes NF-kB and p53 and downregulate the anti-aging gene Nanog. To sum up, the smaller size of ZnO NPs can enhance the aging process in the cells. [Image: see text] Springer US 2021-09-30 2021 /pmc/articles/PMC8484102/ /pubmed/34591206 http://dx.doi.org/10.1007/s10856-021-06602-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biomaterials Synthesis and Characterization
Deylam, Mahla
Alizadeh, Effat
Sarikhani, Manizheh
Hejazy, Marzie
Firouzamandi, Masoumeh
Zinc oxide nanoparticles promote the aging process in a size-dependent manner
title Zinc oxide nanoparticles promote the aging process in a size-dependent manner
title_full Zinc oxide nanoparticles promote the aging process in a size-dependent manner
title_fullStr Zinc oxide nanoparticles promote the aging process in a size-dependent manner
title_full_unstemmed Zinc oxide nanoparticles promote the aging process in a size-dependent manner
title_short Zinc oxide nanoparticles promote the aging process in a size-dependent manner
title_sort zinc oxide nanoparticles promote the aging process in a size-dependent manner
topic Biomaterials Synthesis and Characterization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484102/
https://www.ncbi.nlm.nih.gov/pubmed/34591206
http://dx.doi.org/10.1007/s10856-021-06602-x
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