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Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD

INTRODUCTION: Patients with end-stage renal disease (ESRD) experience disproportionately high cardiovascular morbidity and mortality. Accumulating evidence suggests a role for the circulating microbiome in the pathogenesis of cardiovascular disease; however, little is known about its association wit...

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Autores principales: Sumida, Keiichi, Pierre, Joseph F., Han, Zhongji, Mims, Tahliyah S., Potukuchi, Praveen Kumar, Yuzefpolskaya, Melana, Colombo, Paolo C., Demmer, Ryan T., Datta, Susmita, Kovesdy, Csaba P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484116/
https://www.ncbi.nlm.nih.gov/pubmed/34622101
http://dx.doi.org/10.1016/j.ekir.2021.07.023
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author Sumida, Keiichi
Pierre, Joseph F.
Han, Zhongji
Mims, Tahliyah S.
Potukuchi, Praveen Kumar
Yuzefpolskaya, Melana
Colombo, Paolo C.
Demmer, Ryan T.
Datta, Susmita
Kovesdy, Csaba P.
author_facet Sumida, Keiichi
Pierre, Joseph F.
Han, Zhongji
Mims, Tahliyah S.
Potukuchi, Praveen Kumar
Yuzefpolskaya, Melana
Colombo, Paolo C.
Demmer, Ryan T.
Datta, Susmita
Kovesdy, Csaba P.
author_sort Sumida, Keiichi
collection PubMed
description INTRODUCTION: Patients with end-stage renal disease (ESRD) experience disproportionately high cardiovascular morbidity and mortality. Accumulating evidence suggests a role for the circulating microbiome in the pathogenesis of cardiovascular disease; however, little is known about its association with premature cardiovascular mortality in ESRD. METHODS: In a pilot case-control study of 17 hemodialysis patients who died of a cardiovascular event and 17 matched hemodialysis controls who remained alive during a median follow-up of 2.0 years, we compared the levels and composition of circulating microbiome, including Bacteria, Archaea, and Fungi, in serum samples by quantitative polymerase chain reaction and 16S or Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing, respectively. Associations of the circulating cell-free microbial signatures with clinical parameters and cardiovascular death were examined using the Spearman rank correlation and multivariable conditional logistic regression, respectively. RESULTS: Both 16S and ITS rRNA were detectable in all (except 3 for ITS) examined patients’ serum samples. Despite no significant difference in 16S rRNA levels and α diversity between cases and controls, taxonomic analysis demonstrated differential community membership between groups, with significantly greater Actinobacteria and less Proteobacteria observed in cases than in controls at the phylum level. Proportions of Actinobacteria and Proteobacteria phyla were significantly correlated with plasma nuclear factor erythroid 2−related factor 2 (Nrf2) levels (rho = −0.41 and 0.42, P = 0.015 and 0.013, respectively) and marginally associated with risk of cardiovascular death (adjusted odds ratios [95% confidence intervals] = 1.12 [0.98−1.29] and 0.88 [0.76−1.02] for 1% increase, respectively). CONCLUSION: Alterations of the circulating cell-free microbial signatures may be associated with higher premature cardiovascular mortality in ESRD.
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spelling pubmed-84841162021-10-06 Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD Sumida, Keiichi Pierre, Joseph F. Han, Zhongji Mims, Tahliyah S. Potukuchi, Praveen Kumar Yuzefpolskaya, Melana Colombo, Paolo C. Demmer, Ryan T. Datta, Susmita Kovesdy, Csaba P. Kidney Int Rep Clinical Research INTRODUCTION: Patients with end-stage renal disease (ESRD) experience disproportionately high cardiovascular morbidity and mortality. Accumulating evidence suggests a role for the circulating microbiome in the pathogenesis of cardiovascular disease; however, little is known about its association with premature cardiovascular mortality in ESRD. METHODS: In a pilot case-control study of 17 hemodialysis patients who died of a cardiovascular event and 17 matched hemodialysis controls who remained alive during a median follow-up of 2.0 years, we compared the levels and composition of circulating microbiome, including Bacteria, Archaea, and Fungi, in serum samples by quantitative polymerase chain reaction and 16S or Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing, respectively. Associations of the circulating cell-free microbial signatures with clinical parameters and cardiovascular death were examined using the Spearman rank correlation and multivariable conditional logistic regression, respectively. RESULTS: Both 16S and ITS rRNA were detectable in all (except 3 for ITS) examined patients’ serum samples. Despite no significant difference in 16S rRNA levels and α diversity between cases and controls, taxonomic analysis demonstrated differential community membership between groups, with significantly greater Actinobacteria and less Proteobacteria observed in cases than in controls at the phylum level. Proportions of Actinobacteria and Proteobacteria phyla were significantly correlated with plasma nuclear factor erythroid 2−related factor 2 (Nrf2) levels (rho = −0.41 and 0.42, P = 0.015 and 0.013, respectively) and marginally associated with risk of cardiovascular death (adjusted odds ratios [95% confidence intervals] = 1.12 [0.98−1.29] and 0.88 [0.76−1.02] for 1% increase, respectively). CONCLUSION: Alterations of the circulating cell-free microbial signatures may be associated with higher premature cardiovascular mortality in ESRD. Elsevier 2021-08-04 /pmc/articles/PMC8484116/ /pubmed/34622101 http://dx.doi.org/10.1016/j.ekir.2021.07.023 Text en © 2021 International Society of Nephrology. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Clinical Research
Sumida, Keiichi
Pierre, Joseph F.
Han, Zhongji
Mims, Tahliyah S.
Potukuchi, Praveen Kumar
Yuzefpolskaya, Melana
Colombo, Paolo C.
Demmer, Ryan T.
Datta, Susmita
Kovesdy, Csaba P.
Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD
title Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD
title_full Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD
title_fullStr Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD
title_full_unstemmed Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD
title_short Circulating Microbial Signatures and Cardiovascular Death in Patients With ESRD
title_sort circulating microbial signatures and cardiovascular death in patients with esrd
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484116/
https://www.ncbi.nlm.nih.gov/pubmed/34622101
http://dx.doi.org/10.1016/j.ekir.2021.07.023
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