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5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord
Knee osteoarthritis (KOA) is a common and disabling condition characterized by attacks of pain around the joints, and it is a typical disease that develops chronic pain. Previous studies have proved that 5-HT(1), 5-HT(2), and 5-HT(3) receptors in the spinal cord are involved in electroacupuncture (E...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484641/ https://www.ncbi.nlm.nih.gov/pubmed/34602973 http://dx.doi.org/10.3389/fnins.2021.733779 |
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author | Yuan, Xiao-Cui Yan, Xiang-Ji Tian, Li-Xia Guo, Yi-Xiao Zhao, Yu-Long Baba, Sani Sa’idu Wang, Yu-Ying Liang, Ling-Li Jia, Hong Xu, Lin-Ping Li, Li Lin, Han Huo, Fu-Quan |
author_facet | Yuan, Xiao-Cui Yan, Xiang-Ji Tian, Li-Xia Guo, Yi-Xiao Zhao, Yu-Long Baba, Sani Sa’idu Wang, Yu-Ying Liang, Ling-Li Jia, Hong Xu, Lin-Ping Li, Li Lin, Han Huo, Fu-Quan |
author_sort | Yuan, Xiao-Cui |
collection | PubMed |
description | Knee osteoarthritis (KOA) is a common and disabling condition characterized by attacks of pain around the joints, and it is a typical disease that develops chronic pain. Previous studies have proved that 5-HT(1), 5-HT(2), and 5-HT(3) receptors in the spinal cord are involved in electroacupuncture (EA) analgesia. The 5-HT(7) receptor plays antinociceptive role in the spinal cord. However, it is unclear whether the 5-HT(7) receptor is involved in EA analgesia. The 5-HT(7) receptor is a stimulatory G-protein (Gs)-coupled receptor that activates adenylyl cyclase (AC) to stimulate cyclic adenosine monophosphate (cAMP) formation, which in turn activates protein kinase A (PKA). In the present study, we found that EA significantly increased the tactile threshold and the expression of the 5-HT(7) receptor in the dorsal spinal cord. Intrathecal injection of 5-HT(7) receptor agonist AS-19 mimicked the analgesic effect of EA, while a selective 5-HT(7) receptor antagonist reversed this effect. Moreover, intrathecal injection of AC and PKA antagonists prior to EA intervention prevented its anti-allodynic effect. In addition, GABA(A) receptor antagonist bicuculline administered (intrathecal, i.t.) prior to EA intervention blocked the EA effect on pain hypersensitivity. Our data suggest that the spinal 5-HT(7) receptor activates GABAergic neurons through the Gs–cAMP–PKA pathway and participates in EA-mediated inhibition of chronic pain in a mouse model of KOA. |
format | Online Article Text |
id | pubmed-8484641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84846412021-10-02 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord Yuan, Xiao-Cui Yan, Xiang-Ji Tian, Li-Xia Guo, Yi-Xiao Zhao, Yu-Long Baba, Sani Sa’idu Wang, Yu-Ying Liang, Ling-Li Jia, Hong Xu, Lin-Ping Li, Li Lin, Han Huo, Fu-Quan Front Neurosci Neuroscience Knee osteoarthritis (KOA) is a common and disabling condition characterized by attacks of pain around the joints, and it is a typical disease that develops chronic pain. Previous studies have proved that 5-HT(1), 5-HT(2), and 5-HT(3) receptors in the spinal cord are involved in electroacupuncture (EA) analgesia. The 5-HT(7) receptor plays antinociceptive role in the spinal cord. However, it is unclear whether the 5-HT(7) receptor is involved in EA analgesia. The 5-HT(7) receptor is a stimulatory G-protein (Gs)-coupled receptor that activates adenylyl cyclase (AC) to stimulate cyclic adenosine monophosphate (cAMP) formation, which in turn activates protein kinase A (PKA). In the present study, we found that EA significantly increased the tactile threshold and the expression of the 5-HT(7) receptor in the dorsal spinal cord. Intrathecal injection of 5-HT(7) receptor agonist AS-19 mimicked the analgesic effect of EA, while a selective 5-HT(7) receptor antagonist reversed this effect. Moreover, intrathecal injection of AC and PKA antagonists prior to EA intervention prevented its anti-allodynic effect. In addition, GABA(A) receptor antagonist bicuculline administered (intrathecal, i.t.) prior to EA intervention blocked the EA effect on pain hypersensitivity. Our data suggest that the spinal 5-HT(7) receptor activates GABAergic neurons through the Gs–cAMP–PKA pathway and participates in EA-mediated inhibition of chronic pain in a mouse model of KOA. Frontiers Media S.A. 2021-09-17 /pmc/articles/PMC8484641/ /pubmed/34602973 http://dx.doi.org/10.3389/fnins.2021.733779 Text en Copyright © 2021 Yuan, Yan, Tian, Guo, Zhao, Baba, Wang, Liang, Jia, Xu, Li, Lin and Huo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Yuan, Xiao-Cui Yan, Xiang-Ji Tian, Li-Xia Guo, Yi-Xiao Zhao, Yu-Long Baba, Sani Sa’idu Wang, Yu-Ying Liang, Ling-Li Jia, Hong Xu, Lin-Ping Li, Li Lin, Han Huo, Fu-Quan 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord |
title | 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord |
title_full | 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord |
title_fullStr | 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord |
title_full_unstemmed | 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord |
title_short | 5-HT(7) Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord |
title_sort | 5-ht(7) receptor is involved in electroacupuncture inhibition of chronic pain in the spinal cord |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484641/ https://www.ncbi.nlm.nih.gov/pubmed/34602973 http://dx.doi.org/10.3389/fnins.2021.733779 |
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