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The Application of Microfluidic Technologies in Aptamer Selection
Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligan...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484746/ https://www.ncbi.nlm.nih.gov/pubmed/34604229 http://dx.doi.org/10.3389/fcell.2021.730035 |
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author | Liu, Yang Wang, Nijia Chan, Chiu-Wing Lu, Aiping Yu, Yuanyuan Zhang, Ge Ren, Kangning |
author_facet | Liu, Yang Wang, Nijia Chan, Chiu-Wing Lu, Aiping Yu, Yuanyuan Zhang, Ge Ren, Kangning |
author_sort | Liu, Yang |
collection | PubMed |
description | Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided. |
format | Online Article Text |
id | pubmed-8484746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84847462021-10-02 The Application of Microfluidic Technologies in Aptamer Selection Liu, Yang Wang, Nijia Chan, Chiu-Wing Lu, Aiping Yu, Yuanyuan Zhang, Ge Ren, Kangning Front Cell Dev Biol Cell and Developmental Biology Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided. Frontiers Media S.A. 2021-09-17 /pmc/articles/PMC8484746/ /pubmed/34604229 http://dx.doi.org/10.3389/fcell.2021.730035 Text en Copyright © 2021 Liu, Wang, Chan, Lu, Yu, Zhang and Ren. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Liu, Yang Wang, Nijia Chan, Chiu-Wing Lu, Aiping Yu, Yuanyuan Zhang, Ge Ren, Kangning The Application of Microfluidic Technologies in Aptamer Selection |
title | The Application of Microfluidic Technologies in Aptamer Selection |
title_full | The Application of Microfluidic Technologies in Aptamer Selection |
title_fullStr | The Application of Microfluidic Technologies in Aptamer Selection |
title_full_unstemmed | The Application of Microfluidic Technologies in Aptamer Selection |
title_short | The Application of Microfluidic Technologies in Aptamer Selection |
title_sort | application of microfluidic technologies in aptamer selection |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484746/ https://www.ncbi.nlm.nih.gov/pubmed/34604229 http://dx.doi.org/10.3389/fcell.2021.730035 |
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