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Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis
Colorectal cancer (CRC) is a common malignancy with significant prevalence and mortality rates. Circular RNA FOXO3 (circ-FOXO3; hsa_circ_0006404) has been reported to be involved in cancer regulation; however, its role in CRC is yet to be fully elucidated. Therefore, the aim of the present study was...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8485021/ https://www.ncbi.nlm.nih.gov/pubmed/34549306 http://dx.doi.org/10.3892/or.2021.8190 |
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author | Deng, Yun-Yao Min, Yu-Juan Zhou, Kun Yang, Qing-Song Peng, Mei Cui, Zhao-Rui Zhu, Xiang-Lian Liu, Hao Wang, Min Zhang, Xie Liu, Li-Xin |
author_facet | Deng, Yun-Yao Min, Yu-Juan Zhou, Kun Yang, Qing-Song Peng, Mei Cui, Zhao-Rui Zhu, Xiang-Lian Liu, Hao Wang, Min Zhang, Xie Liu, Li-Xin |
author_sort | Deng, Yun-Yao |
collection | PubMed |
description | Colorectal cancer (CRC) is a common malignancy with significant prevalence and mortality rates. Circular RNA FOXO3 (circ-FOXO3; hsa_circ_0006404) has been reported to be involved in cancer regulation; however, its role in CRC is yet to be fully elucidated. Therefore, the aim of the present study was to investigate the effect of circ-FOXO3 on CRC progression and identify its underlying mechanism. In the present study, the expression of circ-FOXO3 was investigated in CRC tissues and cells via reverse transcription-quantitative PCR. A Cell Counting Kit-8 and colony formation assays were used to assess cell proliferation. The cell migratory and invasive abilities were detected using the Transwell migration and invasion assays. The luciferase assay and RNA pull-down assay were conducted to verify the relationship of circ-FOXO3, microRNA (miR)-543 and Large tumor suppressor kinase 1 (LATS1). The results demonstrated that circ-FOXO3 expression was downregulated in CRC tissues and cells, and was associated with poor overall survival of patients with CRC. Moreover, circ-FOXO3 was associated with tumor size, distant metastasis, differentiation, lymph node metastasis and TMN stages of patients with CRC. circ-FOXO3 overexpression suppressed CRC cell proliferation, migration and invasion. Luciferase assay and RNA pull-down assay results indicated that circ-FOXO3 functioned as a sponge for miR-543. In addition, circ-FOXO3 increased the expression of LATS1 via sponging miR-543, thus inhibiting CRC cell aggressive features. Collectively, the present results suggested that circ-FOXO3 inhibited CRC metastasis and progression via elevated LATS1 expression by sponging miR-543. Therefore, circ-FOXO3 may be a promising target for CRC therapy. |
format | Online Article Text |
id | pubmed-8485021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84850212021-10-07 Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis Deng, Yun-Yao Min, Yu-Juan Zhou, Kun Yang, Qing-Song Peng, Mei Cui, Zhao-Rui Zhu, Xiang-Lian Liu, Hao Wang, Min Zhang, Xie Liu, Li-Xin Oncol Rep Articles Colorectal cancer (CRC) is a common malignancy with significant prevalence and mortality rates. Circular RNA FOXO3 (circ-FOXO3; hsa_circ_0006404) has been reported to be involved in cancer regulation; however, its role in CRC is yet to be fully elucidated. Therefore, the aim of the present study was to investigate the effect of circ-FOXO3 on CRC progression and identify its underlying mechanism. In the present study, the expression of circ-FOXO3 was investigated in CRC tissues and cells via reverse transcription-quantitative PCR. A Cell Counting Kit-8 and colony formation assays were used to assess cell proliferation. The cell migratory and invasive abilities were detected using the Transwell migration and invasion assays. The luciferase assay and RNA pull-down assay were conducted to verify the relationship of circ-FOXO3, microRNA (miR)-543 and Large tumor suppressor kinase 1 (LATS1). The results demonstrated that circ-FOXO3 expression was downregulated in CRC tissues and cells, and was associated with poor overall survival of patients with CRC. Moreover, circ-FOXO3 was associated with tumor size, distant metastasis, differentiation, lymph node metastasis and TMN stages of patients with CRC. circ-FOXO3 overexpression suppressed CRC cell proliferation, migration and invasion. Luciferase assay and RNA pull-down assay results indicated that circ-FOXO3 functioned as a sponge for miR-543. In addition, circ-FOXO3 increased the expression of LATS1 via sponging miR-543, thus inhibiting CRC cell aggressive features. Collectively, the present results suggested that circ-FOXO3 inhibited CRC metastasis and progression via elevated LATS1 expression by sponging miR-543. Therefore, circ-FOXO3 may be a promising target for CRC therapy. D.A. Spandidos 2021-11 2021-09-20 /pmc/articles/PMC8485021/ /pubmed/34549306 http://dx.doi.org/10.3892/or.2021.8190 Text en Copyright: © Deng et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Deng, Yun-Yao Min, Yu-Juan Zhou, Kun Yang, Qing-Song Peng, Mei Cui, Zhao-Rui Zhu, Xiang-Lian Liu, Hao Wang, Min Zhang, Xie Liu, Li-Xin Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis |
title | Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis |
title_full | Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis |
title_fullStr | Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis |
title_full_unstemmed | Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis |
title_short | Identification of the tumor-suppressive role of circular RNA-FOXO3 in colorectal cancer via regulation of miR-543/LATS1 axis |
title_sort | identification of the tumor-suppressive role of circular rna-foxo3 in colorectal cancer via regulation of mir-543/lats1 axis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8485021/ https://www.ncbi.nlm.nih.gov/pubmed/34549306 http://dx.doi.org/10.3892/or.2021.8190 |
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