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BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton

Verticillium wilt is caused by the soil‐borne vascular pathogen Verticillium dahliae, and affects a wide range of economically important crops, including upland cotton (Gossypium hirsutum). Previous studies showed that expression levels of BIN2 were significantly down‐regulated during infestation wi...

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Autores principales: Song, Yun, Zhai, Yaohua, Li, Linxuan, Yang, Zhaoen, Ge, Xiaoyang, Yang, Zuoren, Zhang, Chaojun, Li, Fuguang, Ren, Maozhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486250/
https://www.ncbi.nlm.nih.gov/pubmed/34036698
http://dx.doi.org/10.1111/pbi.13640
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author Song, Yun
Zhai, Yaohua
Li, Linxuan
Yang, Zhaoen
Ge, Xiaoyang
Yang, Zuoren
Zhang, Chaojun
Li, Fuguang
Ren, Maozhi
author_facet Song, Yun
Zhai, Yaohua
Li, Linxuan
Yang, Zhaoen
Ge, Xiaoyang
Yang, Zuoren
Zhang, Chaojun
Li, Fuguang
Ren, Maozhi
author_sort Song, Yun
collection PubMed
description Verticillium wilt is caused by the soil‐borne vascular pathogen Verticillium dahliae, and affects a wide range of economically important crops, including upland cotton (Gossypium hirsutum). Previous studies showed that expression levels of BIN2 were significantly down‐regulated during infestation with V. dahliae. However, the underlying molecular mechanism of BIN2 in plant regulation against V. dahliae remains enigmatic. Here, we characterized a protein kinase GhBIN2 from Gossypium hirsutum, and identified GhBIN2 as a negative regulator of resistance to V. dahliae. The Verticillium wilt resistance of Arabidopsis and cotton were significantly enhanced when BIN2 was knocked down. Constitutive expression of BIN2 attenuated plant resistance to V. dahliae. We found that BIN2 regulated plant endogenous JA content and influenced the expression of JA‐responsive marker genes. Further analysis revealed that BIN2 interacted with and phosphorylated JAZ family proteins, key repressors of the JA signalling pathway in both Arabidopsis and cotton. Spectrometric analysis and site‐directed mutagenesis showed that BIN2 phosphorylated AtJAZ1 at T196, resulting in the degradation of JAZ proteins. Collectively, these results show that BIN2 interacts with JAZ proteins and plays a negative role in plant resistance to V. dahliae. Thus, BIN2 may be a potential target gene for genetic engineering against Verticillium wilt in crops.
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spelling pubmed-84862502021-10-07 BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton Song, Yun Zhai, Yaohua Li, Linxuan Yang, Zhaoen Ge, Xiaoyang Yang, Zuoren Zhang, Chaojun Li, Fuguang Ren, Maozhi Plant Biotechnol J Research Articles Verticillium wilt is caused by the soil‐borne vascular pathogen Verticillium dahliae, and affects a wide range of economically important crops, including upland cotton (Gossypium hirsutum). Previous studies showed that expression levels of BIN2 were significantly down‐regulated during infestation with V. dahliae. However, the underlying molecular mechanism of BIN2 in plant regulation against V. dahliae remains enigmatic. Here, we characterized a protein kinase GhBIN2 from Gossypium hirsutum, and identified GhBIN2 as a negative regulator of resistance to V. dahliae. The Verticillium wilt resistance of Arabidopsis and cotton were significantly enhanced when BIN2 was knocked down. Constitutive expression of BIN2 attenuated plant resistance to V. dahliae. We found that BIN2 regulated plant endogenous JA content and influenced the expression of JA‐responsive marker genes. Further analysis revealed that BIN2 interacted with and phosphorylated JAZ family proteins, key repressors of the JA signalling pathway in both Arabidopsis and cotton. Spectrometric analysis and site‐directed mutagenesis showed that BIN2 phosphorylated AtJAZ1 at T196, resulting in the degradation of JAZ proteins. Collectively, these results show that BIN2 interacts with JAZ proteins and plays a negative role in plant resistance to V. dahliae. Thus, BIN2 may be a potential target gene for genetic engineering against Verticillium wilt in crops. John Wiley and Sons Inc. 2021-06-11 2021-10 /pmc/articles/PMC8486250/ /pubmed/34036698 http://dx.doi.org/10.1111/pbi.13640 Text en © 2021 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Song, Yun
Zhai, Yaohua
Li, Linxuan
Yang, Zhaoen
Ge, Xiaoyang
Yang, Zuoren
Zhang, Chaojun
Li, Fuguang
Ren, Maozhi
BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton
title BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton
title_full BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton
title_fullStr BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton
title_full_unstemmed BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton
title_short BIN2 negatively regulates plant defence against Verticillium dahliae in Arabidopsis and cotton
title_sort bin2 negatively regulates plant defence against verticillium dahliae in arabidopsis and cotton
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486250/
https://www.ncbi.nlm.nih.gov/pubmed/34036698
http://dx.doi.org/10.1111/pbi.13640
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