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Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea

METHODS: The mice were randomly distributed into four groups: (a) control (CTRL) group, (b) ETEC group, (c) IQW-ETEC group, and (d) IRW-ETEC group. Villus length and crypt depth were measured after hematoxylin and eosin staining. The inflammatory reaction was analyzed via inflammatory cytokines (i.e...

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Autores principales: Liu, Naiyuan, Zhou, Lingsi, Fang, Jun, Jiang, Hongmei, Liu, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486560/
https://www.ncbi.nlm.nih.gov/pubmed/34602857
http://dx.doi.org/10.1155/2021/2752265
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author Liu, Naiyuan
Zhou, Lingsi
Fang, Jun
Jiang, Hongmei
Liu, Gang
author_facet Liu, Naiyuan
Zhou, Lingsi
Fang, Jun
Jiang, Hongmei
Liu, Gang
author_sort Liu, Naiyuan
collection PubMed
description METHODS: The mice were randomly distributed into four groups: (a) control (CTRL) group, (b) ETEC group, (c) IQW-ETEC group, and (d) IRW-ETEC group. Villus length and crypt depth were measured after hematoxylin and eosin staining. The inflammatory reaction was analyzed via inflammatory cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10) using the enzyme-linked immunosorbent assay (ELISA). The microbiota in the colon was sequenced using 16S ribosomal RNA. RESULTS: The villus length decreased, the crypt depth decreased, and the expression of inflammatory cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10) increased due to ETEC. In the IRW-ETEC and IQW-ETEC groups, the Shannon index decreased (P < 0.05). IQW and IRW increased the abundance of Firmicutes, Proteobacteria, Clostridiales, Lachnospiraceae, and Alloprevotella; contrastingly, it decreased the abundance of Epsilonproteobacteria, Erysipelotrichales, Prevotellaceae, and Flavobacteriaceae compared to the ETEC group (P <0.05). CONCLUSION: This study ascertained that the addition of IQW and IRW could alleviate jejunal inflammation and increase microbiota community diversity.
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spelling pubmed-84865602021-10-02 Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea Liu, Naiyuan Zhou, Lingsi Fang, Jun Jiang, Hongmei Liu, Gang Mediators Inflamm Research Article METHODS: The mice were randomly distributed into four groups: (a) control (CTRL) group, (b) ETEC group, (c) IQW-ETEC group, and (d) IRW-ETEC group. Villus length and crypt depth were measured after hematoxylin and eosin staining. The inflammatory reaction was analyzed via inflammatory cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10) using the enzyme-linked immunosorbent assay (ELISA). The microbiota in the colon was sequenced using 16S ribosomal RNA. RESULTS: The villus length decreased, the crypt depth decreased, and the expression of inflammatory cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10) increased due to ETEC. In the IRW-ETEC and IQW-ETEC groups, the Shannon index decreased (P < 0.05). IQW and IRW increased the abundance of Firmicutes, Proteobacteria, Clostridiales, Lachnospiraceae, and Alloprevotella; contrastingly, it decreased the abundance of Epsilonproteobacteria, Erysipelotrichales, Prevotellaceae, and Flavobacteriaceae compared to the ETEC group (P <0.05). CONCLUSION: This study ascertained that the addition of IQW and IRW could alleviate jejunal inflammation and increase microbiota community diversity. Hindawi 2021-09-23 /pmc/articles/PMC8486560/ /pubmed/34602857 http://dx.doi.org/10.1155/2021/2752265 Text en Copyright © 2021 Naiyuan Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Naiyuan
Zhou, Lingsi
Fang, Jun
Jiang, Hongmei
Liu, Gang
Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea
title Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea
title_full Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea
title_fullStr Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea
title_full_unstemmed Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea
title_short Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea
title_sort effects of iqw and irw on inflammation and gut microbiota in etec-induced diarrhea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486560/
https://www.ncbi.nlm.nih.gov/pubmed/34602857
http://dx.doi.org/10.1155/2021/2752265
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