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Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable seco...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486745/ https://www.ncbi.nlm.nih.gov/pubmed/34599246 http://dx.doi.org/10.1038/s41598-021-99066-6 |
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author | Poursalavati, Abdonaser Rashidi-Monfared, Sajad Ebrahimi, Amin |
author_facet | Poursalavati, Abdonaser Rashidi-Monfared, Sajad Ebrahimi, Amin |
author_sort | Poursalavati, Abdonaser |
collection | PubMed |
description | Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway. |
format | Online Article Text |
id | pubmed-8486745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84867452021-10-04 Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss Poursalavati, Abdonaser Rashidi-Monfared, Sajad Ebrahimi, Amin Sci Rep Article Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway. Nature Publishing Group UK 2021-10-01 /pmc/articles/PMC8486745/ /pubmed/34599246 http://dx.doi.org/10.1038/s41598-021-99066-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Poursalavati, Abdonaser Rashidi-Monfared, Sajad Ebrahimi, Amin Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
title | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
title_full | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
title_fullStr | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
title_full_unstemmed | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
title_short | Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss |
title_sort | toward understanding of the methoxylated flavonoid biosynthesis pathway in dracocephalum kotschyi boiss |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486745/ https://www.ncbi.nlm.nih.gov/pubmed/34599246 http://dx.doi.org/10.1038/s41598-021-99066-6 |
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